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HAMAP rule MF_01965

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General rule information [?]

Accession MF_01965
Dates 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 14)
Name NADHX_dehydratase
Scope(s) Bacteria
Template(s) P94368 (NNRD_BACSU); P31806 (NNR_ECOLI); Q9X024 (NNR_THEMA); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_01965 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NNRD
Protein name RecName: Full=ADP-dependent (S)-NAD(P)H-hydrate dehydratase;
AltName: Full=ADP-dependent NAD(P)HX dehydratase;
Gene name Name=nnrD;

Comments [?]

FUNCTIONCatalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
CATALYTIC ACTIVITY Reaction=(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate; Xref=Rhea:RHEA:32223, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57945, ChEBI:CHEBI:64074, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216; EC=;
CATALYTIC ACTIVITY Reaction=(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate; Xref=Rhea:RHEA:32235, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57783, ChEBI:CHEBI:64076, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SIMILARITYBelongs to the NnrD/CARKD family.

Keywords [?]

Gene Ontology [?]

GO:0052855; Molecular function:ADP-dependent NAD(P)H-hydrate dehydratase activity
GO:0046496; Biological process:nicotinamide nucleotide metabolic process

Cross-references [?]

PROSITE PS01049; YJEF_C_1; 1;
PROSITE PS01050; YJEF_C_2; 1;
PROSITE PS51383; YJEF_C_3; 1;
Pfam PF01256; Carb_kinase; 1;
NCBIfam TIGR00196; YjeF_C; 1;

Features [?]

From: NNRD_BACSU (P94368)
Key From To Description Tag Condition FTGroup
BINDING 186 190 /ligand="AMP"
BINDING 42 42 /ligand="(6S)-NADPHX"
BINDING 104 104 /ligand="(6S)-NADPHX"
BINDING 149 149 /ligand="(6S)-NADPHX"
BINDING 215 215 /ligand="AMP"
BINDING 216 216 /ligand="(6S)-NADPHX"

Additional information [?]

Size range 257-304 amino acids
Related rules None
Fusion Nter: MF_01966 (nrrE) Cter: None

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