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Annotation rule MF_01987
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General rule information [?]

Accession MF_01987
Dates 29-FEB-2016 (Created)
19-NOV-2019 (Last updated, Version 6)
Name Ribokinase
Scope
Bacteria
Archaea
Templates P0A9J6 (RBSK_ECOLI); A0A0H2WZY4 (RBSK_STAAC): [Recover all]
case <OC:Bacteria> or <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RBSK
Protein name
RecName: Full=Ribokinase;
Short=RK;
EC 2.7.1.15;
Gene name
rbsK

Comments [?]

Function Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
Catalytic activity RHEA:13697: ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)
EC 2.7.1.15
Cofactor Mg(2+)
Note: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.
Activity regulation Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
Pathway Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0004747; Molecular function: ribokinase activity.
GO:0046835; Biological process: carbohydrate phosphorylation.
GO:0019303; Biological process: D-ribose catabolic process.

Cross-references [?]

PROSITE PS00584; PFKB_KINASES_2; 1;
Pfam PF00294; PfkB; 1;
PRINTS PR00990; RIBOKINASE; 1;
TIGRFAMs TIGR02152; D_ribokin_bact; 1;

Features [?]

From: RBSK_ECOLI (P0A9J6)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     223     228       ATP     x(2)-G-x(2)-G  
NP_BIND     254     255       ATP     G-D  
REGION     14     16       Substrate binding     x(2)-D  
REGION     42     46       Substrate binding     G-[KR]-[GAS]-x-[NR]  
ACT_SITE     255     255       Proton acceptor     D  
METAL (Optional)     249     249       Potassium     [DN]  
METAL     251     251       Potassium; via carbonyl oxygen        
METAL     285     285       Potassium; via carbonyl oxygen        
METAL     288     288       Potassium; via carbonyl oxygen        
METAL (Optional)     290     290       Potassium; via carbonyl oxygen     G  
METAL (Optional)     294     294       Potassium     S  
BINDING (Optional)     143     143       Substrate     E  
BINDING     187     187       ATP     N  
BINDING     255     255       Substrate     D  
BINDING (Optional)     279     279       ATP     [HN]  

Additional information [?]

Size range 259-382 amino acids
Related rules None
Fusion None