HAMAP rule MF_02005
General rule information
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| Accession | MF_02005 |
| Dates | 7-FEB-2006 (Created)
1-JUN-2023 (Last updated, Version 18) |
| Name | Val_tRNA_synth_type2 |
| Scope(s) |
Bacteria Actinomycetota Rickettsiales Archaea |
| Template(s) | P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE); [ Recover all ] |
| Triggered by |
HAMAP; MF_02005 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYV |
| Protein name | RecName: Full=Valine--tRNA ligase; EC=6.1.1.9; AltName: Full=Valyl-tRNA synthetase; Short=ValRS; |
| Gene name | Name=valS; |
Comments
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| FUNCTION | Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner. |
| CATALYTIC ACTIVITY | Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl- tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA- COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762, ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9; |
| case <OC:Bacteria> | |
| SUBUNIT | Monomer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily. |
Keywords
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Gene Ontology
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| GO:0004832; Molecular function:valine-tRNA ligase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0006438; Biological process:valyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| Pfam | PF00133; tRNA-synt_1; 1; |
| Pfam | PF08264; Anticodon_1; 1; |
| PRINTS | PR00986; TRNASYNTHVAL; 1; |
| NCBIfam | TIGR00422; ValS; 1; |
Features
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| From: SYV_RICPR (Q9ZCN6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 46 | 56 | /note="'HIGH' region" | P-[TYF]-x-[STA]-G-x-x-H-[ILMVP]-G-[HN] | ||||||||
| MOTIF | 536 | 540 | /note="'KMSKS' region" | [KRA]-M-S-K-S | ||||||||
| BINDING | 539 | 539 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 751-914 amino acids |
| Related rules |
MF_02004 |
| Fusion | Nter: None Cter: None |