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Annotation rule MF_02005 |
Accession | MF_02005 |
Dates | 7-FEB-2006 (Created) 20-NOV-2019 (Last updated, Version 14) |
Name | Val_tRNA_synth_type2 |
Scope | Bacteria; Actinobacteria
Bacteria; Rickettsiales
Archaea |
Templates | P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner. |
Catalytic activity | RHEA:10704: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)
EC 6.1.1.9 |
Subunit | Monomer. |
Subcellular location | Cytoplasm. |
Domain | ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site. |
Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily. |
PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Pfam | PF00133; tRNA-synt_1; 1; |
PF08264; Anticodon_1; 1; | |
PRINTS | PR00986; TRNASYNTHVAL; 1; |
TIGRFAMs | TIGR00422; ValS; 1; |
From: SYV_RICPR (Q9ZCN6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF | 46 | 56 | 'HIGH' region | P-[TYF]-x-[STA]-G-x-x-H-[ILMVP]-G-[HN] | ||||||||
MOTIF | 536 | 540 | 'KMSKS' region | [KRA]-M-S-K-S | ||||||||
BINDING | 539 | 539 | ATP | K |
Size range | 751-914 amino acids |
Related rules | MF_02004 (SYV) |
Fusion | None |