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HAMAP rule MF_02016

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General rule information [?]

Accession MF_02016
Dates 15-OCT-2008 (Created)
26-JAN-2023 (Last updated, Version 14)
Name MltF
Scope(s) Bacteria
Template(s) P0AGC5 (MLTF_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_02016 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MLTF
Protein name RecName: Full=Membrane-bound lytic murein transglycosylase F;
AltName: Full=Murein lyase F;
                 Flags: Precursor;
Gene name Name=mltF;

Comments [?]

FUNCTIONMurein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.
CATALYTIC ACTIVITY Reaction=Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.; EC=4.2.2.n1;
SUBCELLULAR LOCATIONCell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane.
DOMAINThe N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.
SIMILARITYIn the N-terminal section; belongs to the bacterial solute- binding protein 3 family.
SIMILARITYIn the C-terminal section; belongs to the transglycosylase Slt family.

Keywords [?]

Gene Ontology [?]

GO:0016998; Biological process:cell wall macromolecule catabolic process
GO:0008933; Molecular function:lytic transglycosylase activity
GO:0009279; Cellular component:cell outer membrane

Cross-references [?]

Pfam PF00497; SBP_bac_3; 1;
Pfam PF01464; SLT; 1;
General Signal; -; 1;

Features [?]

Key From To Description Tag Condition FTGroup
REGION 22 269 /note="Non-LT domain"
REGION 270 Cter /note="LT domain"
ACT_SITE 314 314 E

Additional information [?]

Size range 450-537 amino acids
Related rules None
Fusion Nter: None Cter: None

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