HAMAP rule MF_02019
General rule information
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| Accession | MF_02019 |
| Dates | 16-FEB-2015 (Created)
1-JUN-2023 (Last updated, Version 8) |
| Name | MurF |
| Scope(s) |
Bacteria |
| Template(s) | P11880 (MURF_ECOLI); Q2FWH4 (MURF_STAA8); [ Recover all ] |
| Triggered by |
HAMAP; MF_02019 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MURF |
| Protein name | RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; EC=6.3.2.10; AltName: Full=D-alanyl-D-alanine-adding enzyme; |
| Gene name | Name=murF; |
Comments
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| FUNCTION | Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein. |
| case <OC:Lactobacillales> or <OC:Staphylococcus> | |
| CATALYTIC ACTIVITY | Reaction=ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L- alanyl-gamma-D-glutamyl-L-lysine = ADP + H(+) + phosphate + UDP-N- acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D- alanine; Xref=Rhea:RHEA:16085, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57822, ChEBI:CHEBI:70758, ChEBI:CHEBI:83903, ChEBI:CHEBI:456216; EC=6.3.2.10; |
| else | |
| CATALYTIC ACTIVITY | Reaction=ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L- alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl- meso-2,6-diaminopimeloyl-D-alanyl-D-alanine; Xref=Rhea:RHEA:28374, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57822, ChEBI:CHEBI:61386, ChEBI:CHEBI:83905, ChEBI:CHEBI:456216; EC=6.3.2.10; |
| end case | |
| PATHWAY | Cell wall biogenesis; peptidoglycan biosynthesis. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the MurCDEF family. MurF subfamily. |
Keywords
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| ATP-binding |
| Cell cycle |
| Cell division |
| Cell shape |
| Cell wall biogenesis/degradation |
| Cytoplasm |
| Ligase |
| Nucleotide-binding |
| Peptidoglycan synthesis |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0047480; Molecular function:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity |
| GO:0009252; Biological process:peptidoglycan biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF01225; Mur_ligase; 1; |
| Pfam | PF02875; Mur_ligase_C; 1; |
| Pfam | PF08245; Mur_ligase_M; 1; |
| NCBIfam | TIGR01143; murF; 1; |
Features
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| From: MURF_ECOLI (P11880) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 107 | 113 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-[ST]-x-G-K-T-[ST] | ||||||||
Additional information
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| Size range | 420-535 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |