HAMAP rule MF_02029
General rule information
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Accession | MF_02029 |
Dates | 11-MAY-2015 (Created)
1-JUN-2023 (Last updated, Version 11) |
Name | WecC_RffD |
Scope(s) |
Bacteria Enterobacterales |
Template(s) | P27829 (WECC_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_02029 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | WECC |
Protein name | RecName: Full=UDP-N-acetyl-D-mannosamine dehydrogenase; EC=1.1.1.336; AltName: Full=UDP-ManNAc 6-dehydrogenase; |
Gene name | Name=wecC; |
Comments
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FUNCTION | Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA). |
CATALYTIC ACTIVITY | Reaction=H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate; Xref=Rhea:RHEA:25780, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:68623, ChEBI:CHEBI:70731; EC=1.1.1.336; |
SUBUNIT | Homodimer. |
PATHWAY | Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis. |
SIMILARITY | Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily. |
Keywords
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Gene Ontology
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GO:0089714; Molecular function:UDP-N-acetyl-D-mannosamine dehydrogenase activity |
GO:0009246; Biological process:enterobacterial common antigen biosynthetic process |
Cross-references
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Pfam | PF00984; UDPG_MGDP_dh; 1; |
Pfam | PF03720; UDPG_MGDP_dh_C; 1; |
Pfam | PF03721; UDPG_MGDP_dh_N; 1; |
NCBIfam | TIGR03026; NDP-sugDHase; 1; |
PIRSF | PIRSF500136; UDP_ManNAc_DH; 1; |
PIRSF | PIRSF000124; UDPglc_GDPman_dh; 1; |
Features
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From: WECC_ECOLI (P27829) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 212 | 212 | /note="Proton donor/acceptor" | K | ||||||||
ACT_SITE | 266 | 266 | /note="Nucleophile" | C | ||||||||
BINDING | 13 | 13 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
Y | ||||||||
BINDING | 14 | 14 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
I | ||||||||
BINDING | 33 | 33 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
D | ||||||||
BINDING | 85 | 85 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
T | ||||||||
BINDING | 126 | 126 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
T | ||||||||
BINDING | 160 | 160 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
R | ||||||||
BINDING | 161 | 161 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
V | ||||||||
BINDING | 212 | 212 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
K | ||||||||
BINDING | 216 | 216 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
N | ||||||||
BINDING | 219 | 219 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
R | ||||||||
BINDING | 250 | 250 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain B" |
H | ||||||||
BINDING | 252 | 252 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain B" |
R | ||||||||
BINDING | 263 | 263 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
G | ||||||||
BINDING | 330 | 330 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
[FY] | ||||||||
BINDING | 331 | 331 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
K | ||||||||
BINDING | 338 | 338 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain B" |
R | ||||||||
BINDING | 416 | 416 | /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A" |
[RK] |
Additional information
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Size range | 418-445 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |