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HAMAP rule MF_02029

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General rule information [?]

Accession MF_02029
Dates 11-MAY-2015 (Created)
1-JUN-2023 (Last updated, Version 11)
Name WecC_RffD
Scope(s) Bacteria
Enterobacterales
Template(s) P27829 (WECC_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_02029 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier WECC
Protein name RecName: Full=UDP-N-acetyl-D-mannosamine dehydrogenase;
                 EC=1.1.1.336;
AltName: Full=UDP-ManNAc 6-dehydrogenase;
Gene name Name=wecC;

Comments [?]

FUNCTIONCatalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA).
CATALYTIC ACTIVITY Reaction=H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate; Xref=Rhea:RHEA:25780, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:68623, ChEBI:CHEBI:70731; EC=1.1.1.336;
SUBUNITHomodimer.
PATHWAYBacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis.
SIMILARITYBelongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily.

Keywords [?]


Gene Ontology [?]

GO:0089714; Molecular function:UDP-N-acetyl-D-mannosamine dehydrogenase activity
GO:0009246; Biological process:enterobacterial common antigen biosynthetic process

Cross-references [?]

Pfam PF00984; UDPG_MGDP_dh; 1;
Pfam PF03720; UDPG_MGDP_dh_C; 1;
Pfam PF03721; UDPG_MGDP_dh_N; 1;
NCBIfam TIGR03026; NDP-sugDHase; 1;
PIRSF PIRSF500136; UDP_ManNAc_DH; 1;
PIRSF PIRSF000124; UDPglc_GDPman_dh; 1;

Features [?]

From: WECC_ECOLI (P27829)
Key From To Description Tag Condition FTGroup
ACT_SITE 212 212 /note="Proton donor/acceptor" K
ACT_SITE 266 266 /note="Nucleophile" C
BINDING 13 13 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
Y
BINDING 14 14 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
I
BINDING 33 33 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
D
BINDING 85 85 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
T
BINDING 126 126 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
T
BINDING 160 160 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
R
BINDING 161 161 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
V
BINDING 212 212 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
K
BINDING 216 216 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
N
BINDING 219 219 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
R
BINDING 250 250 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain B"
H
BINDING 252 252 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain B"
R
BINDING 263 263 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
G
BINDING 330 330 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
[FY]
BINDING 331 331 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
K
BINDING 338 338 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain B"
R
BINDING 416 416 /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
/ligand_id="ChEBI:CHEBI:70731"
/ligand_note="ligand shared between homodimeric partners"
/note="in chain A"
[RK]

Additional information [?]

Size range 418-445 amino acids
Related rules None
Fusion Nter: None Cter: None



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