HAMAP rule MF_02029
General rule information
[?]
| Accession |
MF_02029 |
| Dates |
11-MAY-2015 (Created)
1-JUN-2023 (Last updated, Version 11) |
| Scope |
Bacteria; Enterobacterales |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Protein name |
|
RecName:
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Full=UDP-N-acetyl-D-mannosamine dehydrogenase;
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EC 1.1.1.336;
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AltName:
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Full=UDP-ManNAc 6-dehydrogenase;
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| Function |
Catalyzes the four-electron oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N-acetylmannosaminuronic acid (UDP-ManNAcA). |
| Catalytic activity |
RHEA:25780: H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate
EC 1.1.1.336
|
| Subunit |
Homodimer. |
| Pathway |
Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis. |
| Similarity |
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily. |
GO:0089714; Molecular function: UDP-N-acetyl-D-mannosamine dehydrogenase activity.
GO:0009246; Biological process: enterobacterial common antigen biosynthetic process.
| From: WECC_ECOLI (P27829) |
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Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ACT_SITE
|
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212
|
|
212
|
|
Proton donor/acceptor
|
|
|
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K
|
|
|
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ACT_SITE
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266
|
|
266
|
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Nucleophile
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|
|
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C
|
|
|
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BINDING
|
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13
|
|
13
|
|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
Y
|
|
|
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BINDING
|
|
14
|
|
14
|
|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
I
|
|
|
|
BINDING
|
|
33
|
|
33
|
|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
D
|
|
|
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BINDING
|
|
85
|
|
85
|
|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
T
|
|
|
|
BINDING
|
|
126
|
|
126
|
|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
T
|
|
|
|
BINDING
|
|
160
|
|
160
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
R
|
|
|
|
BINDING
|
|
161
|
|
161
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
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V
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|
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BINDING
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212
|
|
212
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|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
K
|
|
|
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BINDING
|
|
216
|
|
216
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
N
|
|
|
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BINDING
|
|
219
|
|
219
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
R
|
|
|
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BINDING
|
|
250
|
|
250
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|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain B
|
|
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H
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|
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BINDING
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252
|
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252
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/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain B
|
|
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R
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|
|
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BINDING
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263
|
|
263
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
G
|
|
|
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BINDING
|
|
330
|
|
330
|
|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
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[FY]
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|
|
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BINDING
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331
|
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331
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/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
|
|
|
|
K
|
|
|
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BINDING
|
|
338
|
|
338
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|
/ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" /ligand_note="ligand shared between homodimeric partners" /note="in chain B
|
|
|
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R
|
|
|
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BINDING
|
|
416
|
|
416
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|
/ligand="UDP-N-acetyl-alpha-D-mannosaminouronate" /ligand_id="ChEBI:CHEBI:70731" /ligand_note="ligand shared between homodimeric partners" /note="in chain A
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[RK]
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Additional information
[?]
| Size range |
418-445 amino acids |
| Related rules |
None |
| Fusion |
None |
