HAMAP rule MF_02035
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02035 |
| Accession | MF_02035 |
| Dates | 1-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 11) |
| Name | EgtB |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | G7CFI3 (EGTB_MYCT3); A0R5N0 (EGTB_MYCS2); [ Recover all ] |
| Triggered by |
HAMAP; MF_02035 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | EGTB |
| Protein name | RecName: Full=Hercynine oxygenase; EC=1.14.99.50; AltName: Full=Gamma-glutamyl hercynylcysteine S-oxide synthase; |
| Gene name | Name=egtB; |
Comments
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| FUNCTION | Catalyzes the oxidative sulfurization of hercynine (N- alpha,N-alpha,N-alpha-trimethyl-L-histidine) into hercynyl-gamma-L- glutamyl-L-cysteine sulfoxide, a step in the biosynthesis pathway of ergothioneine. |
| CATALYTIC ACTIVITY | Reaction=gamma-L-glutamyl-L-cysteine + hercynine + O2 = gamma-L- glutamyl-hercynylcysteine S-oxide + H2O; Xref=Rhea:RHEA:42672, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:15781, ChEBI:CHEBI:58173, ChEBI:CHEBI:82703; EC=1.14.99.50; |
| COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; |
| PATHWAY | Amino-acid biosynthesis; ergothioneine biosynthesis. |
| SIMILARITY | Belongs to the EgtB family. |
Keywords
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Gene Ontology
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| GO:0005506; Molecular function:iron ion binding |
| GO:0016705; Molecular function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| GO:0052699; Biological process:ergothioneine biosynthetic process |
Cross-references
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Features
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| From: EGTB_MYCT3 (G7CFI3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 87 | 90 | /ligand="gamma-L-glutamyl-L-cysteine" /ligand_id="ChEBI:CHEBI:58173" |
R-x-x-R | ||||||||
| BINDING | 51 | 51 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 134 | 134 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 138 | 138 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 416 | 416 | /ligand="gamma-L-glutamyl-L-cysteine" /ligand_id="ChEBI:CHEBI:58173" |
D | ||||||||
| BINDING | 420 | 420 | /ligand="gamma-L-glutamyl-L-cysteine" /ligand_id="ChEBI:CHEBI:58173" |
R | ||||||||
Additional information
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| Size range | 414-510 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |