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HAMAP rule MF_02042

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General rule information [?]

Accession MF_02042
Dates 20-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 7)
Name DusB_subfam
Scope(s) Bacteria
Template(s) P0ABT5 (DUSB_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_02042 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DUSB
Protein name RecName: Full=tRNA-dihydrouridine synthase B;
Gene name Name=dusB;

Comments [?]

FUNCTIONCatalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
CATALYTIC ACTIVITY Reaction=a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH; Xref=Rhea:RHEA:54452, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CATALYTIC ACTIVITY Reaction=a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH; Xref=Rhea:RHEA:23624, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
SIMILARITYBelongs to the Dus family. DusB subfamily.

Keywords [?]

Gene Ontology [?]

GO:0010181; Molecular function:FMN binding
GO:0000049; Molecular function:tRNA binding
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity
GO:0002943; Biological process:tRNA dihydrouridine synthesis

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
NCBIfam TIGR00737; nifR3_yhdG; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 16 18 /ligand="FMN"
BINDING 200 202 /ligand="FMN"
BINDING 224 225 /ligand="FMN"
ACT_SITE 100 100 /note="Proton donor" C
BINDING 70 70 /ligand="FMN"
BINDING 139 139 /ligand="FMN"

Additional information [?]

Size range 305-400 amino acids
Related rules None
Fusion Nter: None Cter: None

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