HAMAP rule MF_02042
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02042 |
| Accession | MF_02042 |
| Dates | 20-JUL-2015 (Created)
3-SEP-2024 (Last updated, Version 8) |
| Name | DusB_subfam |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P0ABT5 (DUSB_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_02042 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DUSB |
| Protein name | RecName: Full=tRNA-dihydrouridine synthase B; EC=1.3.1.-; |
| Gene name | Name=dusB; |
Comments
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| FUNCTION | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. |
| CATALYTIC ACTIVITY | Reaction=a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + NADH + H(+); Xref=Rhea:RHEA:54452, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
| CATALYTIC ACTIVITY | Reaction=a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + NADPH + H(+); Xref=Rhea:RHEA:23624, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
| SIMILARITY | Belongs to the Dus family. DusB subfamily. |
Keywords
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Gene Ontology
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| GO:0010181; Molecular function:FMN binding |
| GO:0000049; Molecular function:tRNA binding |
| GO:0017150; Molecular function:tRNA dihydrouridine synthase activity |
| GO:0002943; Biological process:tRNA dihydrouridine synthesis |
Cross-references
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| PROSITE | PS01136; UPF0034; 1; |
| Pfam | PF01207; Dus; 1; |
| NCBIfam | TIGR00737; nifR3_yhdG; 1; |
| PIRSF | PIRSF006621; Dus; 1; |
Features
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| From: DUSB_ECOLI (P0ABT5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 16 | 18 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
P-M-A | ||||||||
| BINDING | 200 | 202 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
N-G-D | ||||||||
| BINDING | 224 | 225 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
G-R | ||||||||
| ACT_SITE | 100 | 100 | /note="Proton donor" | C | ||||||||
| BINDING | 70 | 70 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q | ||||||||
| BINDING | 139 | 139 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
K | ||||||||
Additional information
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| Size range | 305-400 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |