HAMAP rule MF_02042
General rule information
[?]
Accession | MF_02042 |
Dates | 20-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 7) |
Name | DusB_subfam |
Scope(s) |
Bacteria Pseudomonadota |
Template(s) | P0ABT5 (DUSB_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_02042 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier | DUSB |
Protein name | RecName: Full=tRNA-dihydrouridine synthase B; EC=1.3.1.-; |
Gene name | Name=dusB; |
Comments
[?]
FUNCTION | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. |
CATALYTIC ACTIVITY | Reaction=a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH; Xref=Rhea:RHEA:54452, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
CATALYTIC ACTIVITY | Reaction=a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH; Xref=Rhea:RHEA:23624, Rhea:RHEA-COMP:13339, Rhea:RHEA- COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
SIMILARITY | Belongs to the Dus family. DusB subfamily. |
Keywords
[?]
Gene Ontology
[?]
GO:0010181; Molecular function:FMN binding |
GO:0000049; Molecular function:tRNA binding |
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity |
GO:0002943; Biological process:tRNA dihydrouridine synthesis |
Cross-references
[?]
PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
NCBIfam | TIGR00737; nifR3_yhdG; 1; |
PIRSF | PIRSF006621; Dus; 1; |
Features
[?]
From: DUSB_ECOLI (P0ABT5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 16 | 18 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
P-M-A | ||||||||
BINDING | 200 | 202 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
N-G-D | ||||||||
BINDING | 224 | 225 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
G-R | ||||||||
ACT_SITE | 100 | 100 | /note="Proton donor" | C | ||||||||
BINDING | 70 | 70 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q | ||||||||
BINDING | 139 | 139 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
K |
Additional information
[?]
Size range | 305-400 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |