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Annotation rule MF_02042
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General rule information [?]

Accession MF_02042
Dates 20-JUL-2015 (Created)
20-NOV-2019 (Last updated, Version 3)
Name DusB_subfam
Scope
Bacteria; Proteobacteria
Template P0ABT5 (DUSB_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DUSB
Protein name
RecName: Full=tRNA-dihydrouridine synthase B;
EC 1.3.1.-;
Gene name
dusB

Comments [?]

Function Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity RHEA:54452: a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH
RHEA:23624: a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH
Cofactor FMN
Similarity Belongs to the Dus family. DusB subfamily.

Keywords [?]

Flavoprotein
FMN
NADP
Oxidoreductase
RNA-binding
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
TIGRFAMs TIGR00737; nifR3_yhdG; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

From: DUSB_ECOLI (P0ABT5)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     16     18       FMN     P-M-A  
NP_BIND (Optional)     200     202       FMN     N-G-D  
NP_BIND (Optional)     224     225       FMN     G-R  
ACT_SITE     100     100       Proton donor     C  
BINDING (Optional)     70     70       FMN     Q  
BINDING (Optional)     139     139       FMN     K  

Additional information [?]

Size range 305-400 amino acids
Related rules None
Fusion None


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