HAMAP rule MF_02042

General rule information [?]

Accession	MF_02042
Dates	20-JUL-2015 (Created) 1-JUN-2023 (Last updated, Version 7)

Name	DusB_subfam

Scope

Bacteria; Pseudomonadota

Template

P0ABT5 (DUSB_ECOLI)

Triggered by

HAMAP; MF_02042 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DUSB

Protein name

RecName:		Full=tRNA-dihydrouridine synthase B;
		EC 1.3.1.-;

Gene name

dusB

Comments [?]

Function	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity	RHEA:54452: a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH
	RHEA:23624: a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH
Cofactor	FMN
Similarity	Belongs to the Dus family. DusB subfamily.

Keywords [?]

Flavoprotein
FMN
NADP
Oxidoreductase
RNA-binding
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE	PS01136; UPF0034; 1;
Pfam	PF01207; Dus; 1;
NCBIfam	TIGR00737; nifR3_yhdG; 1;
PIRSF	PIRSF006621; Dus; 1;

Features [?]

From: DUSB_ECOLI (P0ABT5)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 16 18 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 P-M-A

BINDING (Optional) 200 202 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 N-G-D

BINDING (Optional) 224 225 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 G-R

ACT_SITE 100 100 Proton donor C

BINDING (Optional) 70 70 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 Q

BINDING (Optional) 139 139 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 K

Additional information [?]

Size range	305-400 amino acids
Related rules	None
Fusion	None

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