HAMAP rule MF_02042
General rule information
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Accession | MF_02042 |
Dates | 20-JUL-2015 (Created) 1-JUN-2023 (Last updated, Version 7) |
Name | DusB_subfam |
Scope | Bacteria; Pseudomonadota |
Template | P0ABT5 (DUSB_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. |
Catalytic activity | RHEA:54452: a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH |
RHEA:23624: a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH | |
Cofactor | FMN |
Similarity | Belongs to the Dus family. DusB subfamily. |
Keywords
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Gene Ontology
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GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.
Cross-references
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PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
NCBIfam | TIGR00737; nifR3_yhdG; 1; |
PIRSF | PIRSF006621; Dus; 1; |
Features
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From: DUSB_ECOLI (P0ABT5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING (Optional) | 16 | 18 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | P-M-A | ||||||||
BINDING (Optional) | 200 | 202 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | N-G-D | ||||||||
BINDING (Optional) | 224 | 225 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | G-R | ||||||||
ACT_SITE | 100 | 100 | Proton donor | C | ||||||||
BINDING (Optional) | 70 | 70 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | Q | ||||||||
BINDING (Optional) | 139 | 139 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | K |
Additional information
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Size range | 305-400 amino acids |
Related rules | None |
Fusion | None |