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HAMAP rule MF_02042

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General rule information [?]

Accession MF_02042
Dates 20-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 7)
Name DusB_subfam
Bacteria; Pseudomonadota
Template P0ABT5 (DUSB_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=tRNA-dihydrouridine synthase B;
EC 1.3.1.-;
Gene name

Comments [?]

Function Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Catalytic activity RHEA:54452: a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH
RHEA:23624: a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH
Cofactor FMN
Similarity Belongs to the Dus family. DusB subfamily.

Keywords [?]

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
NCBIfam TIGR00737; nifR3_yhdG; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     16     18       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     P-M-A  
BINDING (Optional)     200     202       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     N-G-D  
BINDING (Optional)     224     225       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     G-R  
ACT_SITE     100     100       Proton donor     C  
BINDING (Optional)     70     70       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     Q  
BINDING (Optional)     139     139       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     K  

Additional information [?]

Size range 305-400 amino acids
Related rules None
Fusion None