HAMAP rule MF_02059

General rule information [?]

Accession	MF_02059
Dates	11-MAR-2016 (Created) 26-JAN-2023 (Last updated, Version 10)

Name	Activ_enz_CutD

Scope

Bacteria; Coriobacteriales

Bacteria; Bacillota

Bacteria; Pseudomonadota

Template

Q30W71 (CUTD_OLEA2)

Triggered by

HAMAP; MF_02059 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

CUTD

Protein name

RecName:		Full=Choline trimethylamine-lyase activating enzyme;
		EC 1.97.1.-;
AltName:		Full=Choline utilization protein D;
AltName:		Full=GRE activase CutD;
AltName:		Full=Glycyl-radical enzyme activating enzyme CutD;
		Short=GRE activating enzyme CutD;

Gene name

cutD

Comments [?]

Function	Catalyzes activation of the choline trimethylamine-lyase CutC under anaerobic conditions by generation of an organic free radical on a glycine residue, via an homolytic cleavage of S-adenosyl-L-methionine (SAM).
Catalytic activity	RHEA:61976: glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]

case <FTGroup:1> and <FTGroup:2>

Cofactor

[4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine;

else

Cofactor

[4Fe-4S] cluster

end case

Pathway	Amine and polyamine metabolism; choline degradation.
Similarity	Belongs to the organic radical-activating enzymes family.

Keywords [?]

4Fe-4S
Iron
Iron-sulfur
Metal-binding
Oxidoreductase
Repeat
S-adenosyl-L-methionine

Gene Ontology [?]

GO:0016491; Molecular function: oxidoreductase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0042426; Biological process: choline catabolic process.

Cross-references [?]

PROSITE	PS00198; 4FE4S_FER_1; 2;
	PS51379; 4FE4S_FER_2; 2; trigger=PRU00711;
	PS01087; RADICAL_ACTIVATING; 1;
Pfam	PF04055; Radical_SAM; 1;
TIGRFAMs	TIGR04395; cutC_activ_rSAM; 1;
	TIGR02494; PFLE_PFLC; 1;
PIRSF	PIRSF000371; PFL_act_enz; 1;

Features [?]

From: CUTD_OLEA2 (Q30W71)

Key From To Description Tag Condition FTGroup

BINDING 37 39 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 W-C-[SA]

BINDING 188 190 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 D-[VLIM]-K

BINDING 31 31 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 35 35 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 38 38 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 57 57 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING 60 60 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING 63 63 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING 99 99 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING 139 139 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 G

BINDING 264 264 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 H

Additional information [?]

Size range	300-360 amino acids
Related rules	None
Fusion	None

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