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HAMAP rule MF_02059

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General rule information [?]

Accession MF_02059
Dates 11-MAR-2016 (Created)
1-JUN-2023 (Last updated, Version 11)
Name Activ_enz_CutD
Scope(s) Bacteria
Template(s) Q30W71 (CUTD_OLEA2); [ Recover all ]
Triggered by HAMAP; MF_02059 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CUTD
Protein name RecName: Full=Choline trimethylamine-lyase activating enzyme;
AltName: Full=Choline utilization protein D;
AltName: Full=GRE activase CutD;
AltName: Full=Glycyl-radical enzyme activating enzyme CutD;
                 Short=GRE activating enzyme CutD;
Gene name Name=cutD;

Comments [?]

FUNCTIONCatalyzes activation of the choline trimethylamine-lyase CutC under anaerobic conditions by generation of an organic free radical on a glycine residue, via an homolytic cleavage of S-adenosyl-L-methionine (SAM).
CATALYTIC ACTIVITY Reaction=glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L- methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]; Xref=Rhea:RHEA:61976, Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:14480, Rhea:RHEA-COMP:15993, Rhea:RHEA-COMP:15994, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29947, ChEBI:CHEBI:32722, ChEBI:CHEBI:57618, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:140311;
case <FTGroup:1> and <FTGroup:2>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
PATHWAYAmine and polyamine metabolism; choline degradation.
SIMILARITYBelongs to the organic radical-activating enzymes family.

Keywords [?]

Gene Ontology [?]

GO:0016491; Molecular function:oxidoreductase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0042426; Biological process:choline catabolic process

Cross-references [?]

PROSITE PS00198; 4FE4S_FER_1; 2;
PROSITE PS51379; 4FE4S_FER_2; 2;
Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR04395; cutC_activ_rSAM; 1;
NCBIfam TIGR02494; PFLE_PFLC; 1;
PIRSF PIRSF000371; PFL_act_enz; 1;

Features [?]

From: CUTD_OLEA2 (Q30W71)
Key From To Description Tag Condition FTGroup
BINDING 37 39 /ligand="S-adenosyl-L-methionine"
BINDING 188 190 /ligand="S-adenosyl-L-methionine"
BINDING 31 31 /ligand="[4Fe-4S] cluster"
C 1
BINDING 35 35 /ligand="[4Fe-4S] cluster"
C 1
BINDING 38 38 /ligand="[4Fe-4S] cluster"
C 1
BINDING 57 57 /ligand="[4Fe-4S] cluster"
C 2
BINDING 60 60 /ligand="[4Fe-4S] cluster"
C 2
BINDING 63 63 /ligand="[4Fe-4S] cluster"
C 2
BINDING 99 99 /ligand="[4Fe-4S] cluster"
C 2
BINDING 139 139 /ligand="S-adenosyl-L-methionine"
BINDING 264 264 /ligand="S-adenosyl-L-methionine"

Additional information [?]

Size range 300-360 amino acids
Related rules None
Fusion Nter: None Cter: None

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