HAMAP rule MF_02059

General rule information [?]

Accession	MF_02059
Dates	11-MAR-2016 (Created) 1-JUN-2023 (Last updated, Version 11)
Name	Activ_enz_CutD
Scope(s)	Bacteria Coriobacteriales Bacillota Pseudomonadota
Template(s)	Q30W71 (CUTD_OLEA2); [ Recover all ]
Triggered by	HAMAP; MF_02059 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CUTD
Protein name	RecName: Full=Choline trimethylamine-lyase activating enzyme; EC=1.97.1.-; AltName: Full=Choline utilization protein D; AltName: Full=GRE activase CutD; AltName: Full=Glycyl-radical enzyme activating enzyme CutD; Short=GRE activating enzyme CutD;
Gene name	Name=cutD;

Comments [?]

FUNCTION	Catalyzes activation of the choline trimethylamine-lyase CutC under anaerobic conditions by generation of an organic free radical on a glycine residue, via an homolytic cleavage of S-adenosyl-L-methionine (SAM).
CATALYTIC ACTIVITY	Reaction=glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L- methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]; Xref=Rhea:RHEA:61976, Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:14480, Rhea:RHEA-COMP:15993, Rhea:RHEA-COMP:15994, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29947, ChEBI:CHEBI:32722, ChEBI:CHEBI:57618, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:140311;
case <FTGroup:1> and <FTGroup:2>
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine;
else
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
PATHWAY	Amine and polyamine metabolism; choline degradation.
SIMILARITY	Belongs to the organic radical-activating enzymes family.

Keywords [?]

Gene Ontology [?]

GO:0016491; Molecular function:oxidoreductase activity

GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding

GO:0042426; Biological process:choline catabolic process

Cross-references [?]

Features [?]

From: CUTD_OLEA2 (Q30W71)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	37	39	/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789"		W-C-[SA]
BINDING	188	190	/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789"		D-[VLIM]-K
BINDING	31	31	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet"		C	1
BINDING	35	35	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet"		C	1
BINDING	38	38	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet"		C	1
BINDING	57	57	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	60	60	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	63	63	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	99	99	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	139	139	/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789"		G
BINDING	264	264	/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789"		H

Additional information [?]