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Annotation rule MF_02059
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General rule information [?]

Accession MF_02059
Dates 11-MAR-2016 (Created)
27-JAN-2020 (Last updated, Version 3)
Name Activ_enz_CutD
Scope
Bacteria; Coriobacteriales
Bacteria; Firmicutes
Bacteria; Proteobacteria
Template Q30W71 (CUTD_DESAG)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CUTD
Protein name
RecName: Full=Choline trimethylamine-lyase activating enzyme;
EC 1.97.1.-;
AltName: Full=Choline utilization protein D;
AltName: Full=GRE activase CutD;
AltName: Full=Glycyl-radical enzyme activating enzyme CutD;
Short=GRE activating enzyme CutD;
Gene name
cutD

Comments [?]

Function Catalyzes activation of the choline trimethylamine-lyase CutC under anaerobic conditions by generation of an organic free radical on a glycine residue, via an homolytic cleavage of S-adenosyl-L-methionine (SAM).
Catalytic activity RHEA:61976: glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]
case <FTGroup:1> and <FTGroup:2>
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine;
else
Cofactor [4Fe-4S] cluster
end case
Pathway Amine and polyamine metabolism; choline degradation.
Similarity Belongs to the organic radical-activating enzymes family.

Keywords [?]

4Fe-4S
Iron
Iron-sulfur
Metal-binding
Oxidoreductase
Repeat
S-adenosyl-L-methionine

Gene Ontology [?]

GO:0016491; Molecular function: oxidoreductase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0042426; Biological process: choline catabolic process.

Cross-references [?]

PROSITE PS00198; 4FE4S_FER_1; 2;
PS51379; 4FE4S_FER_2; 2; trigger=PRU00711;
PS01087; RADICAL_ACTIVATING; 1;
Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR04395; cutC_activ_rSAM; 1;
TIGR02494; PFLE_PFLC; 1;
PIRSF PIRSF000371; PFL_act_enz; 1;

Features [?]

From: CUTD_DESAG (Q30W71)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     37     39       S-adenosyl-L-methionine binding     W-C-[SA]  
REGION     188     190       S-adenosyl-L-methionine binding     D-[VLIM]-K  
METAL     31     31       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C   1
METAL     35     35       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C   1
METAL     38     38       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C   1
METAL     57     57       Iron-sulfur 2 (4Fe-4S)     C   2
METAL     60     60       Iron-sulfur 2 (4Fe-4S)     C   2
METAL     63     63       Iron-sulfur 2 (4Fe-4S)     C   2
METAL     99     99       Iron-sulfur 2 (4Fe-4S)     C   2
BINDING     139     139       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     264     264       S-adenosyl-L-methionine; via carbonyl oxygen and amide nitrogen     H  

Additional information [?]

Size range 300-360 amino acids
Related rules None
Fusion None


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