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Annotation rule MF_02067
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General rule information [?]

Accession MF_02067
Dates 30-JUN-2016 (Created)
20-NOV-2019 (Last updated, Version 4)
Name FBP_aldolase_phosphatase
Scope
Bacteria
Archaea
Templates F9VMT6 (FBPAP_SULTO); B1YAL1 (FBPAP_PYRNV); Q2RG86 (FBPAP_MOOTA); Q72K02 (FBPAP_THET2); A0RV30 (FBPAP_CENSY); A8A9E4 (FBPAP_IGNH4); A4YIZ5 (FBPAP_METS5); D9PUH5 (FBPAP_METTM); Q8NKR9 (FBPAP_THEKO): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
FBPAP
Protein name
RecName: Full=Fructose-1,6-bisphosphate aldolase/phosphatase;
Short=FBP A/P;
Short=FBP aldolase/phosphatase;
EC 3.1.3.11;
EC 4.1.2.13;
Gene name
fbp

Comments [?]

Function Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).
Catalytic activity RHEA:11064: beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate
EC 3.1.3.11
RHEA:14729: beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate
EC 4.1.2.13
Cofactor Mg(2+)
Pathway Carbohydrate biosynthesis; gluconeogenesis.
Subunit Homooctamer; dimer of tetramers.
Domain Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.
Similarity Belongs to the FBP aldolase/phosphatase family.

Keywords [?]


Gene Ontology [?]

GO:0042132; Molecular function: fructose 1,6-bisphosphate 1-phosphatase activity.
GO:0004332; Molecular function: fructose-bisphosphate aldolase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006094; Biological process: gluconeogenesis.

Cross-references [?]

Pfam PF01950; FBPase_3; 1;
PIRSF PIRSF015647; FBPtase_archl; 1;

Features [?]

From: FBPAP_SULTO (F9VMT6)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     103     104       FBP binding     G-N  
REGION (Optional)     241     242       FBP binding; shared with dimeric partner     Q-[HSK]  
ACT_SITE     11     11       Proton acceptor; for FBP phosphatase activity     D  
ACT_SITE     228     228       Proton donor/acceptor; for FBP aldolase activity     Y  
ACT_SITE     231     231       Schiff-base intermediate with DHAP; for FBP aldolase activity     K  
METAL     11     11       Magnesium 1     D  
METAL     18     18       Magnesium 1; via pros nitrogen     H  
METAL     52     52       Magnesium 1     D  
METAL     52     52       Magnesium 2     D  
METAL     53     53       Magnesium 2     D  
METAL     94     94       Magnesium 1     Q  
METAL     131     131       Magnesium 2     D  
METAL     231     231       Magnesium 3; via carbonyl oxygen     K  
METAL     232     232       Magnesium 3     D  
METAL     232     232       Magnesium 4     D  
METAL     233     233       Magnesium 2     D  
METAL     233     233       Magnesium 3     D  
BINDING (Optional)     18     18       FBP; via tele nitrogen     H  
BINDING (Optional)     90     90       FBP     Y  
BINDING (Optional)     132     132       FBP/DHAP     K  
BINDING (Optional)     265     265       FBP/DHAP; via amide nitrogen     R  
BINDING (Optional)     286     286       FBP/DHAP     D  
BINDING (Optional)     347     347       FBP     Y  

Additional information [?]

Size range 360-410 amino acids
Related rules None
Fusion None