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| Annotation rule MF_02067 |
General rule information
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| Accession | MF_02067 |
| Dates | 30-JUN-2016 (Created) 20-NOV-2019 (Last updated, Version 4) |
| Name | FBP_aldolase_phosphatase |
| Scope | Bacteria
Archaea |
| Templates | F9VMT6 (FBPAP_SULTO); B1YAL1 (FBPAP_PYRNV); Q2RG86 (FBPAP_MOOTA); Q72K02 (FBPAP_THET2); A0RV30 (FBPAP_CENSY); A8A9E4 (FBPAP_IGNH4); A4YIZ5 (FBPAP_METS5); D9PUH5 (FBPAP_METTM); Q8NKR9 (FBPAP_THEKO): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P). |
| Catalytic activity | RHEA:11064: beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate
EC 3.1.3.11 |
| RHEA:14729: beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate
EC 4.1.2.13 |
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| Cofactor | Mg(2+) |
| Pathway | Carbohydrate biosynthesis; gluconeogenesis. |
| Subunit | Homooctamer; dimer of tetramers. |
| Domain | Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities. |
| Similarity | Belongs to the FBP aldolase/phosphatase family. |
Keywords
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Gene Ontology
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GO:0042132; Molecular function: fructose 1,6-bisphosphate 1-phosphatase activity.
GO:0004332; Molecular function: fructose-bisphosphate aldolase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006094; Biological process: gluconeogenesis.
GO:0004332; Molecular function: fructose-bisphosphate aldolase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006094; Biological process: gluconeogenesis.
Cross-references
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| Pfam | PF01950; FBPase_3; 1; |
| PIRSF | PIRSF015647; FBPtase_archl; 1; |
Features
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| From: FBPAP_SULTO (F9VMT6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION (Optional) | 103 | 104 | FBP binding | G-N | ||||||||
| REGION (Optional) | 241 | 242 | FBP binding; shared with dimeric partner | Q-[HSK] | ||||||||
| ACT_SITE | 11 | 11 | Proton acceptor; for FBP phosphatase activity | D | ||||||||
| ACT_SITE | 228 | 228 | Proton donor/acceptor; for FBP aldolase activity | Y | ||||||||
| ACT_SITE | 231 | 231 | Schiff-base intermediate with DHAP; for FBP aldolase activity | K | ||||||||
| METAL | 11 | 11 | Magnesium 1 | D | ||||||||
| METAL | 18 | 18 | Magnesium 1; via pros nitrogen | H | ||||||||
| METAL | 52 | 52 | Magnesium 1 | D | ||||||||
| METAL | 52 | 52 | Magnesium 2 | D | ||||||||
| METAL | 53 | 53 | Magnesium 2 | D | ||||||||
| METAL | 94 | 94 | Magnesium 1 | Q | ||||||||
| METAL | 131 | 131 | Magnesium 2 | D | ||||||||
| METAL | 231 | 231 | Magnesium 3; via carbonyl oxygen | K | ||||||||
| METAL | 232 | 232 | Magnesium 3 | D | ||||||||
| METAL | 232 | 232 | Magnesium 4 | D | ||||||||
| METAL | 233 | 233 | Magnesium 2 | D | ||||||||
| METAL | 233 | 233 | Magnesium 3 | D | ||||||||
| BINDING (Optional) | 18 | 18 | FBP; via tele nitrogen | H | ||||||||
| BINDING (Optional) | 90 | 90 | FBP | Y | ||||||||
| BINDING (Optional) | 132 | 132 | FBP/DHAP | K | ||||||||
| BINDING (Optional) | 265 | 265 | FBP/DHAP; via amide nitrogen | R | ||||||||
| BINDING (Optional) | 286 | 286 | FBP/DHAP | D | ||||||||
| BINDING (Optional) | 347 | 347 | FBP | Y | ||||||||
Additional information
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| Size range | 360-410 amino acids |
| Related rules | None |
| Fusion | None |