HAMAP rule MF_02067
General rule information
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Accession | MF_02067 |
Dates | 30-JUN-2016 (Created)
19-NOV-2022 (Last updated, Version 9) |
Name | FBP_aldolase_phosphatase |
Scope(s) |
Bacteria Archaea |
Template(s) | F9VMT6 (FBPAP_SULTO); B1YAL1 (FBPAP_PYRNV); Q2RG86 (FBPAP_MOOTA); Q72K02 (FBPAP_THET2); A0RV30 (FBPAP_CENSY); A8A9E4 (FBPAP_IGNH4); A4YIZ5 (FBPAP_METS5); D9PUH5 (FBPAP_METTM); Q8NKR9 (FBPAP_THEKO); [ Recover all ] |
Triggered by |
HAMAP; MF_02067 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | FBPAP |
Protein name | RecName: Full=Fructose-1,6-bisphosphate aldolase/phosphatase; Short=FBP A/P; Short=FBP aldolase/phosphatase; EC=3.1.3.11; EC=4.1.2.13; |
Gene name | Name=fbp; |
Comments
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FUNCTION | Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3- phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P). |
CATALYTIC ACTIVITY | Reaction=beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6- phosphate + phosphate; Xref=Rhea:RHEA:11064, ChEBI:CHEBI:15377, ChEBI:CHEBI:32966, ChEBI:CHEBI:43474, ChEBI:CHEBI:57634; EC=3.1.3.11; |
CATALYTIC ACTIVITY | Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3- phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729, ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
PATHWAY | Carbohydrate biosynthesis; gluconeogenesis. |
SUBUNIT | Homooctamer; dimer of tetramers. |
DOMAIN | Consists of a single catalytic domain, but remodels its active- site architecture via a large structural change to exhibit dual activities. |
SIMILARITY | Belongs to the FBP aldolase/phosphatase family. |
Keywords
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Gene Ontology
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GO:0042132; Molecular function:fructose 1,6-bisphosphate 1-phosphatase activity |
GO:0004332; Molecular function:fructose-bisphosphate aldolase activity |
GO:0000287; Molecular function:magnesium ion binding |
GO:0006094; Biological process:gluconeogenesis |
Cross-references
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Pfam | PF01950; FBPase_3; 1; |
PIRSF | PIRSF015647; FBPtase_archl; 1; |
Features
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From: FBPAP_SULTO (F9VMT6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 103 | 104 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
G-N | ||||||||
BINDING | 241 | 242 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" |
Q-[HSK] | ||||||||
ACT_SITE | 11 | 11 | /note="Proton acceptor; for FBP phosphatase activity" | D | ||||||||
ACT_SITE | 228 | 228 | /note="Proton donor/acceptor; for FBP aldolase activity" | Y | ||||||||
ACT_SITE | 231 | 231 | /note="Schiff-base intermediate with DHAP; for FBP aldolase activity" | K | ||||||||
BINDING | 11 | 11 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
BINDING | 18 | 18 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
H | ||||||||
BINDING | 52 | 52 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
BINDING | 52 | 52 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 53 | 53 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 94 | 94 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
Q | ||||||||
BINDING | 131 | 131 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 231 | 231 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
K | ||||||||
BINDING | 232 | 232 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
D | ||||||||
BINDING | 232 | 232 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" |
D | ||||||||
BINDING | 233 | 233 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
BINDING | 233 | 233 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" |
D | ||||||||
BINDING | 18 | 18 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
H | ||||||||
BINDING | 18 | 18 | /ligand="dihydroxyacetone phosphate" /ligand_id="ChEBI:CHEBI:57642" |
H | ||||||||
BINDING | 90 | 90 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
Y | ||||||||
BINDING | 132 | 132 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
K | ||||||||
BINDING | 132 | 132 | /ligand="dihydroxyacetone phosphate" /ligand_id="ChEBI:CHEBI:57642" |
K | ||||||||
BINDING | 265 | 265 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
R | ||||||||
BINDING | 265 | 265 | /ligand="dihydroxyacetone phosphate" /ligand_id="ChEBI:CHEBI:57642" |
R | ||||||||
BINDING | 286 | 286 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
D | ||||||||
BINDING | 286 | 286 | /ligand="dihydroxyacetone phosphate" /ligand_id="ChEBI:CHEBI:57642" |
D | ||||||||
BINDING | 347 | 347 | /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
Y |
Additional information
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Size range | 360-410 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |