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HAMAP rule MF_02067

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General rule information [?]

Accession MF_02067
Dates 30-JUN-2016 (Created)
19-NOV-2022 (Last updated, Version 9)
Name FBP_aldolase_phosphatase
Scope(s) Bacteria
Archaea
Template(s) F9VMT6 (FBPAP_SULTO); B1YAL1 (FBPAP_PYRNV); Q2RG86 (FBPAP_MOOTA); Q72K02 (FBPAP_THET2); A0RV30 (FBPAP_CENSY); A8A9E4 (FBPAP_IGNH4); A4YIZ5 (FBPAP_METS5); D9PUH5 (FBPAP_METTM); Q8NKR9 (FBPAP_THEKO); [ Recover all ]
Triggered by HAMAP; MF_02067 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FBPAP
Protein name RecName: Full=Fructose-1,6-bisphosphate aldolase/phosphatase;
                 Short=FBP A/P;
                 Short=FBP aldolase/phosphatase;
                 EC=3.1.3.11;
                 EC=4.1.2.13;
Gene name Name=fbp;

Comments [?]

FUNCTIONCatalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3- phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).
CATALYTIC ACTIVITY Reaction=beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6- phosphate + phosphate; Xref=Rhea:RHEA:11064, ChEBI:CHEBI:15377, ChEBI:CHEBI:32966, ChEBI:CHEBI:43474, ChEBI:CHEBI:57634; EC=3.1.3.11;
CATALYTIC ACTIVITY Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3- phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729, ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCarbohydrate biosynthesis; gluconeogenesis.
SUBUNITHomooctamer; dimer of tetramers.
DOMAINConsists of a single catalytic domain, but remodels its active- site architecture via a large structural change to exhibit dual activities.
SIMILARITYBelongs to the FBP aldolase/phosphatase family.

Keywords [?]

Carbohydrate metabolism
Gluconeogenesis
Hydrolase
Lyase
Magnesium
Metal-binding
Schiff base

Gene Ontology [?]

GO:0042132; Molecular function:fructose 1,6-bisphosphate 1-phosphatase activity
GO:0004332; Molecular function:fructose-bisphosphate aldolase activity
GO:0000287; Molecular function:magnesium ion binding
GO:0006094; Biological process:gluconeogenesis

Cross-references [?]

Pfam PF01950; FBPase_3; 1;
PIRSF PIRSF015647; FBPtase_archl; 1;

Features [?]

From: FBPAP_SULTO (F9VMT6)
Key From To Description Tag Condition FTGroup
BINDING 103 104 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 G-N
BINDING 241 242 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"		 Q-[HSK]
ACT_SITE 11 11 /note="Proton acceptor; for FBP phosphatase activity" D
ACT_SITE 228 228 /note="Proton donor/acceptor; for FBP aldolase activity" Y
ACT_SITE 231 231 /note="Schiff-base intermediate with DHAP; for FBP aldolase activity" K
BINDING 11 11 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"		 D
BINDING 18 18 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"		 H
BINDING 52 52 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"		 D
BINDING 52 52 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"		 D
BINDING 53 53 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"		 D
BINDING 94 94 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"		 Q
BINDING 131 131 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"		 D
BINDING 231 231 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"		 K
BINDING 232 232 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"		 D
BINDING 232 232 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="4"		 D
BINDING 233 233 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"		 D
BINDING 233 233 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="3"		 D
BINDING 18 18 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 H
BINDING 18 18 /ligand="dihydroxyacetone phosphate"
/ligand_id="ChEBI:CHEBI:57642"		 H
BINDING 90 90 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 Y
BINDING 132 132 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 K
BINDING 132 132 /ligand="dihydroxyacetone phosphate"
/ligand_id="ChEBI:CHEBI:57642"		 K
BINDING 265 265 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 R
BINDING 265 265 /ligand="dihydroxyacetone phosphate"
/ligand_id="ChEBI:CHEBI:57642"		 R
BINDING 286 286 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 D
BINDING 286 286 /ligand="dihydroxyacetone phosphate"
/ligand_id="ChEBI:CHEBI:57642"		 D
BINDING 347 347 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 Y

Additional information [?]

Size range 360-410 amino acids
Related rules None
Fusion Nter: None Cter: None



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