HAMAP rule MF_02081
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02081 |
| Accession | MF_02081 |
| Dates | 06-DEC-2016 (Created)
14-AUG-2024 (Last updated, Version 9) |
| Name | MrdA_transpept |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P0AD65; D0C8Z9; [ Recover all ] |
| Triggered by |
HAMAP; MF_02081 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MRDA |
| Protein name | RecName: Full=Peptidoglycan D,D-transpeptidase MrdA; EC=3.4.16.4; AltName: Full=Penicillin-binding protein 2; Short=PBP-2; |
| Gene name | Name=mrdA; |
Comments
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| FUNCTION | Catalyzes cross-linking of the peptidoglycan cell wall. |
| CATALYTIC ACTIVITY | Reaction=Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds one Zn(2+) ion per subunit.; |
| end case | |
| PATHWAY | Cell wall biogenesis; peptidoglycan biosynthesis. |
| case <Feature:Transmembrane==1> | |
| SUBCELLULAR LOCATION | Cell inner membrane; Single-pass membrane protein. |
| end case | |
| SIMILARITY | Belongs to the transpeptidase family. MrdA subfamily. |
Keywords
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Gene Ontology
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| GO:0009002; Molecular function:serine-type D-Ala-D-Ala carboxypeptidase activity | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0009252; Biological process:peptidoglycan biosynthetic process | |
| GO:0005886; Cellular component:plasma membrane | |
Cross-references
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| Pfam | PF03717; PBP_dimer; 1; |
| Pfam | PF00905; Transpeptidase; 1; |
| NCBIfam | TIGR03423; pbp2_mrdA; 1; |
| General | Transmembrane; -; 1; |
Features
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| From: MRDA_ECOLI (P0AD65) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 330 | 330 | /note="Acyl-ester intermediate" | S | ||||||||
| From: MRDA_ACIB2 (D0C8Z9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 350 | 350 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
D | 1 | |||||||
| BINDING | 365 | 365 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
D | 1 | |||||||
| BINDING | 371 | 371 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 384 | 384 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
Additional information
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| Size range | 580-810 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |