HAMAP rule MF_02097
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_02097 |
| Accession | MF_02097 |
| Dates | 31-OCT-2017 (Created)
03-SEP-2024 (Last updated, Version 11) |
| Name | Carnitine_monoox_A |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | D0C9N6; F0KFI5; P0ABR7; [ Recover all ] |
| Triggered by |
HAMAP; MF_02097 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | CNTA |
| Protein name | RecName: Full=Carnitine monooxygenase oxygenase subunit; EC=1.14.13.239; AltName: Full=Carnitine monooxygenase alpha subunit; |
Comments
[?]
| FUNCTION | Converts carnitine to trimethylamine and malic semialdehyde. |
| CATALYTIC ACTIVITY | Reaction=(R)-carnitine + NADH + O2 + H(+) = (3R)-3-hydroxy-4- oxobutanoate + trimethylamine + NAD(+) + H2O; Xref=Rhea:RHEA:55396, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16347, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58389, ChEBI:CHEBI:138809; EC=1.14.13.239; |
| CATALYTIC ACTIVITY | Reaction=(R)-carnitine + NADPH + O2 + H(+) = (3R)-3-hydroxy-4- oxobutanoate + trimethylamine + NADP(+) + H2O; Xref=Rhea:RHEA:55368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16347, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58389, ChEBI:CHEBI:138809; EC=1.14.13.239; |
| case <FTGroup:1> | |
| COFACTOR | Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster per subunit.; |
| end case | |
| COFACTOR | Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 1 Fe cation per subunit |
| PATHWAY | Amine and polyamine metabolism; carnitine metabolism. |
| SUBUNIT | Composed of an oxygenase subunit and a reductase subunit. |
| SIMILARITY | Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. CntA subfamily. |
Keywords
[?]
| case <FTGroup:1> | |
| 2Fe-2S | |
| Iron-sulfur | |
| end case | |
| Iron | |
| Metal-binding | |
| NAD | |
| NADP | |
| Oxidoreductase | |
Gene Ontology
[?]
| GO:0016709; Molecular function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
| case <FTGroup:1> | |
| GO:0051537; Molecular function:2 iron, 2 sulfur cluster binding | |
| end case | |
| GO:0009437; Biological process:carnitine metabolic process | |
Cross-references
[?]
| PROSITE | PS51296; RIESKE; 1; |
| PROSITE | PS00570; RING_HYDROXYL_ALPHA; 1; |
| Pfam | PF00355; Rieske; 1; |
| Pfam | PF00848; Ring_hydroxyl_A; 1; |
| PRINTS | PR00090; RNGDIOXGNASE; 1; |
Features
[?]
| From: CNTA_ACIB2 (D0C9N6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 86 | 86 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
| BINDING | 88 | 88 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
H | 1 | |||||||
| BINDING | 106 | 106 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
| BINDING | 109 | 109 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
H | 1 | |||||||
| BINDING | 208 | 208 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 213 | 213 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 323 | 323 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
D | ||||||||
Additional information
[?]
| Size range | 365-405 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | See MF_02098 for the reductase subunit. |