HAMAP rule MF_02106

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_02106
Accession	MF_02106
Dates	07-DEC-2009 (Created) 27-JUL-2023 (Last updated, Version 13)
Name	Pup
Scope(s)	Bacteria Actinomycetota
Template(s)	P9WHN5; A0QZ48; Q8NQE0; [ Recover all ]
Triggered by	HAMAP; MF_02106 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	PUP
Protein name	RecName: Full=Prokaryotic ubiquitin-like protein Pup; AltName: Full=Bacterial ubiquitin-like modifier;
Gene name	Name=pup;

Comments [?]

FUNCTION	Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation.
PATHWAY	Protein degradation; proteasomal Pup-dependent pathway.
SUBUNIT	Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring.
DOMAIN	The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome.
case <FTTag:deamidation>
PTM	Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process.
end case
SIMILARITY	Belongs to the prokaryotic ubiquitin-like protein family.

Keywords [?]

Gene Ontology [?]

GO:0031386; Molecular function:protein tag activity

GO:0070628; Molecular function:proteasome binding

GO:0010498; Biological process:proteasomal protein catabolic process

GO:0019941; Biological process:modification-dependent protein catabolic process

GO:0070490; Biological process:protein pupylation

Cross-references [?]

Pfam	PF05639; Pup; 1;
General	Coiled_coil; -; 0-unlimited;

Features [?]

From: PUP_MYCTU (P9WHN5)

Key

From

Description

Tag

Condition

FTGroup

REGION

/note="ARC ATPase binding"

MOD_RES

/note="Deamidated glutamine"

deamidation

case <FT:2>

CROSSLNK

/note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins)"

[Q]

else

CROSSLNK

/note="Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins)"

[E]

end case

Additional information [?]

Size range	62-72 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process.