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Annotation rule MF_02106 |
Accession | MF_02106 |
Dates | 7-DEC-2009 (Created) 20-NOV-2019 (Last updated, Version 10) |
Name | Pup |
Scope | Bacteria; Actinobacteria |
Templates | P9WHN5 (PUP_MYCTU); A0QZ48 (PUP_MYCS2); Q8NQE0 (PUP_CORGL): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. |
Pathway | Protein degradation; proteasomal Pup-dependent pathway. |
Subunit | Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring. |
Domain | The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome. |
Ptm | Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process. |
Similarity | Belongs to the prokaryotic ubiquitin-like protein family. |
Pfam | PF05639; Pup; 1; |
General | Coiled_coil; -; 0-unlimited; trigger=yes; |
From: PUP_MYCTU (P9WHN5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 21 | 58 | ARC ATPase binding | |||||||||
MOD_RES (Optional) | 64 | 64 | Deamidated glutamine | deamidation | Q |
CROSSLNK | 64 | 64 | Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins) | [Q] |
CROSSLNK | 64 | 64 | Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins) | [E] |
Size range | 62-72 amino acids |
Related rules | None |
Fusion | None |
Comments | Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process. |