HAMAP rule MF_02106
General rule information
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| Accession | MF_02106 |
| Dates | 7-DEC-2009 (Created) 13-JAN-2023 (Last updated, Version 11) |
| Name | Pup |
| Scope | Bacteria; Actinomycetota |
| Templates | P9WHN5 (PUP_MYCTU); A0QZ48 (PUP_MYCS2); Q8NQE0 (PUP_CORGL): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. |
| Pathway | Protein degradation; proteasomal Pup-dependent pathway. |
| Subunit | Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring. |
| Domain | The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome. |
case <FTTag:deamidation>
| Ptm | Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process. |
end case
| Similarity | Belongs to the prokaryotic ubiquitin-like protein family. |
Keywords
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Gene Ontology
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GO:0031386; Molecular function: protein tag.
GO:0070628; Molecular function: proteasome binding.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0070490; Biological process: protein pupylation.
GO:0070628; Molecular function: proteasome binding.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0070490; Biological process: protein pupylation.
Cross-references
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| Pfam | PF05639; Pup; 1; |
Computed features
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| General | Coiled_coil; -; 0-unlimited; trigger=yes; |
Features
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| From: PUP_MYCTU (P9WHN5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 21 | 58 | ARC ATPase binding | |||||||||
| MOD_RES (Optional) | 64 | 64 | Deamidated glutamine | deamidation | Q | |||||||
case <FT:2>
| CROSSLNK | 64 | 64 | Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins) | [Q] |
else
| CROSSLNK | 64 | 64 | Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins) | [E] |
end case
Additional information
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| Size range | 62-72 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process. |