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HAMAP rule MF_02106

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General rule information [?]

Accession MF_02106
Dates 7-DEC-2009 (Created)
27-JUL-2023 (Last updated, Version 12)
Name Pup
Scope(s) Bacteria
Template(s) P9WHN5 (PUP_MYCTU); A0QZ48 (PUP_MYCS2); Q8NQE0 (PUP_CORGL); [ Recover all ]
Triggered by HAMAP; MF_02106 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PUP
Protein name RecName: Full=Prokaryotic ubiquitin-like protein Pup;
AltName: Full=Bacterial ubiquitin-like modifier;
Gene name Name=pup;

Comments [?]

FUNCTIONProtein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation.
PATHWAYProtein degradation; proteasomal Pup-dependent pathway.
SUBUNITStrongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring.
DOMAINThe N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome.
case <FTTag:deamidation>
PTMIs modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process.
end case
SIMILARITYBelongs to the prokaryotic ubiquitin-like protein family.

Keywords [?]

Gene Ontology [?]

GO:0031386; Molecular function:protein tag activity
GO:0070628; Molecular function:proteasome binding
GO:0010498; Biological process:proteasomal protein catabolic process
GO:0019941; Biological process:modification-dependent protein catabolic process
GO:0070490; Biological process:protein pupylation

Cross-references [?]

Pfam PF05639; Pup; 1;
General Coiled_coil; -; 0-unlimited;

Features [?]

Key From To Description Tag Condition FTGroup
REGION 21 58 /note="ARC ATPase binding"
MOD_RES 64 64 /note="Deamidated glutamine" deamidation Q
case <FT:2>
CROSSLNK 64 64 /note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins)" [Q]
CROSSLNK 64 64 /note="Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins)" [E]
end case

Additional information [?]

Size range 62-72 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process.

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