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HAMAP rule MF_02106

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General rule information [?]

Accession MF_02106
Dates 7-DEC-2009 (Created)
13-JAN-2023 (Last updated, Version 11)
Name Pup
Bacteria; Actinomycetota
Templates P9WHN5 (PUP_MYCTU); A0QZ48 (PUP_MYCS2); Q8NQE0 (PUP_CORGL): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Prokaryotic ubiquitin-like protein Pup;
AltName: Full=Bacterial ubiquitin-like modifier;
Gene name

Comments [?]

Function Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation.
Pathway Protein degradation; proteasomal Pup-dependent pathway.
Subunit Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring.
Domain The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome.
case <FTTag:deamidation>
Ptm Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process.
end case
Similarity Belongs to the prokaryotic ubiquitin-like protein family.

Keywords [?]

Gene Ontology [?]

GO:0031386; Molecular function: protein tag.
GO:0070628; Molecular function: proteasome binding.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0070490; Biological process: protein pupylation.

Cross-references [?]

Pfam PF05639; Pup; 1;

Computed features [?]

General Coiled_coil; -; 0-unlimited; trigger=yes;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
REGION     21     58       ARC ATPase binding        
MOD_RES (Optional)     64     64       Deamidated glutamine   deamidation   Q  
case <FT:2>
CROSSLNK     64     64       Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins)     [Q]  
CROSSLNK     64     64       Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins)     [E]  
end case

Additional information [?]

Size range 62-72 amino acids
Related rules None
Fusion None
Comments Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process.