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HAMAP rule MF_02111

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General rule information [?]

Accession MF_02111
Dates 2-JUL-2010 (Created)
1-JUN-2023 (Last updated, Version 18)
Name Pup_ligase
Scope(s) Bacteria
Template(s) P9WNU7 (PAFA_MYCTU); A0QZ42 (PAFA_MYCS2); Q8NQE1 (PAFA_CORGL); [ Recover all ]
Triggered by HAMAP; MF_02111 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PAFA
Protein name RecName: Full=Pup--protein ligase;
AltName: Full=Proteasome accessory factor A;
AltName: Full=Pup-conjugating enzyme;
Gene name Name=pafA;

Comments [?]

FUNCTIONCatalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side- chain amino group of a substrate lysine.
CATALYTIC ACTIVITY Reaction=ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin- like protein]-gamma-L-glutamyl)-[protein]-L-lysine.; EC=;
PATHWAYProtein degradation; proteasomal Pup-dependent pathway.
PATHWAYProtein modification; protein pupylation.
MISCELLANEOUSThe reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.
SIMILARITYBelongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0005524; Molecular function:ATP binding
GO:0019787; Molecular function:ubiquitin-like protein transferase activity
GO:0010498; Biological process:proteasomal protein catabolic process
GO:0019941; Biological process:modification-dependent protein catabolic process
GO:0070490; Biological process:protein pupylation

Cross-references [?]

Pfam PF03136; Pup_ligase; 1;
NCBIfam TIGR03686; Pupylate_PafA; 1;
PIRSF PIRSF018077; UCP018077; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 64 64 /note="Proton acceptor" D
BINDING 16 16 /ligand="Mg(2+)"
BINDING 62 62 /ligand="Mg(2+)"
BINDING 70 70 /ligand="Mg(2+)"
BINDING 60 60 /ligand="ATP"
BINDING 73 73 /ligand="ATP"
BINDING 440 440 /ligand="ATP"

Additional information [?]

Size range 447-504 amino acids
Related rules None
Fusion Nter: None Cter: None

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