HAMAP rule MF_02111
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02111 |
| Accession | MF_02111 |
| Dates | 02-JUL-2010 (Created)
01-JUN-2023 (Last updated, Version 19) |
| Name | Pup_ligase |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | P9WNU7; A0QZ42; Q8NQE1; [ Recover all ] |
| Triggered by |
HAMAP; MF_02111 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PAFA |
| Protein name | RecName: Full=Pup--protein ligase; EC=6.3.1.19; AltName: Full=Proteasome accessory factor A; AltName: Full=Pup-conjugating enzyme; |
| Gene name | Name=pafA; |
Comments
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| FUNCTION | Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side- chain amino group of a substrate lysine. |
| CATALYTIC ACTIVITY | Reaction=ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin- like protein]-gamma-L-glutamyl)-[protein]-L-lysine.; EC=6.3.1.19; |
| PATHWAY | Protein degradation; proteasomal Pup-dependent pathway. |
| PATHWAY | Protein modification; protein pupylation. |
| MISCELLANEOUS | The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group. |
| SIMILARITY | Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0005524; Molecular function:ATP binding |
| GO:0019787; Molecular function:ubiquitin-like protein transferase activity |
| GO:0010498; Biological process:proteasomal protein catabolic process |
| GO:0019941; Biological process:modification-dependent protein catabolic process |
| GO:0070490; Biological process:protein pupylation |
Cross-references
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| Pfam | PF03136; Pup_ligase; 1; |
| NCBIfam | TIGR03686; Pupylate_PafA; 1; |
| PIRSF | PIRSF018077; UCP018077; 1; |
Features
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| From: PAFA_CORGL (Q8NQE1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 64 | 64 | /note="Proton acceptor" | D | ||||||||
| BINDING | 16 | 16 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 62 | 62 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
Y | ||||||||
| BINDING | 70 | 70 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 60 | 60 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
| BINDING | 73 | 73 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[TS] | ||||||||
| BINDING | 440 | 440 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
W | ||||||||
Additional information
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| Size range | 447-504 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |