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HAMAP rule MF_02111

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General rule information [?]

Accession MF_02111
Dates 2-JUL-2010 (Created)
13-JAN-2023 (Last updated, Version 17)
Name Pup_ligase
Bacteria; Actinomycetota
Templates P9WNU7 (PAFA_MYCTU); A0QZ42 (PAFA_MYCS2); Q8NQE1 (PAFA_CORGL): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Pup--protein ligase;
AltName: Full=Proteasome accessory factor A;
AltName: Full=Pup-conjugating enzyme;
Gene name

Comments [?]

Function Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Catalytic activity Reaction=ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.; EC=;
Pathway Protein degradation; proteasomal Pup-dependent pathway.
Protein modification; protein pupylation.
Miscellaneous The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.
Similarity Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0019787; Molecular function: ubiquitin-like protein transferase activity.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0070490; Biological process: protein pupylation.

Cross-references [?]

Pfam PF03136; Pup_ligase; 1;
TIGRFAMs TIGR03686; Pupylate_PafA; 1;
PIRSF PIRSF018077; UCP018077; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     64     64       Proton acceptor     D  
BINDING     16     16       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E  
BINDING     62     62       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     Y  
BINDING     70     70       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E  
BINDING     60     60       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R  
BINDING     73     73       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [TS]  
BINDING     440     440       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     W  

Additional information [?]

Size range 447-504 amino acids
Related rules None
Fusion None