HAMAP rule MF_02116
General rule information
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| Accession | MF_02116 |
| Dates | 22-FEB-2011 (Created)
19-NOV-2022 (Last updated, Version 10) |
| Name | FAPy_deform |
| Scope(s) |
Archaea Methanobacteria Methanococci |
| Template(s) | Q57580 (ARFB_METJA); [ Recover all ] |
| Triggered by |
HAMAP; MF_02116 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ARFB |
| Protein name | RecName: Full=2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase; Short=FAPy deformylase; EC=3.5.1.102; AltName: Full=Formamide hydrolase; |
| Gene name | Name=arfB; |
Comments
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| FUNCTION | Catalyzes the hydrolysis of the formamide of 2-amino-5- formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-monophosphate (FAPy) to form 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (APy). |
| CATALYTIC ACTIVITY | Reaction=2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin- 4(3H)-one + H2O = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate + H(+); Xref=Rhea:RHEA:27282, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57258, ChEBI:CHEBI:59545; EC=3.5.1.102; |
| COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Requires one Fe(2+) ion for activity.; |
| COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Requires an additional second metal ion that could be Fe(2+) or Zn(2+).; |
| PATHWAY | Cofactor biosynthesis; coenzyme F420 biosynthesis. |
| PATHWAY | Cofactor biosynthesis; riboflavin biosynthesis. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the creatininase superfamily. FAPy deformylase family. |
Keywords
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Gene Ontology
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| GO:0008198; Molecular function:ferrous iron binding |
| GO:0016811; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| GO:0009231; Biological process:riboflavin biosynthetic process |
| GO:0052645; Biological process:F420-0 metabolic process |
Cross-references
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| Pfam | PF02633; Creatininase; 1; |
Features
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| From: ARFB_METJA (Q57580) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 28 | 28 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
E | ||||||||
| BINDING | 30 | 30 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
H | ||||||||
| BINDING | 39 | 39 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
D | ||||||||
| BINDING | 39 | 39 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
D | ||||||||
| BINDING | 107 | 107 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
H | ||||||||
Additional information
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| Size range | 221-260 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |