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Annotation rule MF_02128
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General rule information [?]

Accession MF_02128
Dates 3-JAN-2012 (Created)
20-NOV-2019 (Last updated, Version 7)
Name TMP_kinase
Scope
Bacteria
Archaea
Templates O67883 (THIL_AQUAE); P55881 (THIL_SALTY); P0AGG0 (THIL_ECOLI); A3MTW6 (THIL_PYRCJ): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THIL
Protein name
RecName: Full=Thiamine-monophosphate kinase;
Short=TMP kinase;
Short=Thiamine-phosphate kinase;
EC 2.7.4.16;
Gene name
thiL

Comments [?]

Function Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.
Catalytic activity RHEA:15913: ATP + thiamine phosphate = ADP + thiamine diphosphate
EC 2.7.4.16
Pathway Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.
Miscellaneous Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.
Similarity Belongs to the thiamine-monophosphate kinase family.

Keywords [?]

ATP-binding
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Thiamine biosynthesis
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0009030; Molecular function: thiamine-phosphate kinase activity.
GO:0009229; Biological process: thiamine diphosphate biosynthetic process.

Cross-references [?]

Pfam PF00586; AIRS; 1;
PF02769; AIRS_C; 1;
TIGRFAMs TIGR01379; ThiL; 1;
PIRSF PIRSF005303; Thiam_monoph_kin; 1;

Features [?]

From: THIL_AQUAE (O67883)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     118     119       ATP     G-[ND]  
METAL     27     27       Magnesium 3     [DE]  
METAL     27     27       Magnesium 4; via carbonyl oxygen     [DE]  
METAL     41     41       Magnesium 4     [TS]  
METAL     42     42       Magnesium 1; via carbonyl oxygen     [TS]  
METAL     43     43       Magnesium 1     D  
METAL     43     43       Magnesium 2     D  
METAL     71     71       Magnesium 2     D  
METAL     71     71       Magnesium 3     D  
METAL     71     71       Magnesium 4     D  
METAL     119     119       Magnesium 1     [ND]  
METAL     207     207       Magnesium 3     D  
METAL     210     210       Magnesium 5     D  
BINDING     50     50       Substrate     [HD]  
BINDING (Optional)     101     101       ATP     Y  
BINDING (Optional)     142     142       ATP     [RK]  
BINDING     209     209       ATP     [ST]  
BINDING     260     260       Substrate     [ED]  
BINDING     303     303       Substrate     [WYF]  

Additional information [?]

Size range 286-352 amino acids
Related rules None
Fusion None


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