HAMAP rule MF_02205
General rule information
[?]
| Accession | MF_02205 |
| Dates | 13-FEB-2018 (Created)
22-APR-2024 (Last updated, Version 11) |
| Name | DinG_proteobact |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P27296 (DING_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_02205 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | DING |
| RecName: Full=ATP-dependent DNA helicase DinG;<br /> EC=<a href="https://enzyme.expasy.org/EC/5.6.2.3">5.6.2.3</a>;<br /> AltName: Full=DNA 5'-3' helicase DinG; | |
| Gene name | Name=dinG; |
Comments
[?]
| FUNCTION | DNA-dependent ATPase and 5'-3' DNA helicase. Unwinds D-loops, R-loops, forked DNA and G-quadruplex DNA. |
| CATALYTIC ACTIVITY | Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.; EC=5.6.2.3; |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.3; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster |
| SIMILARITY | Belongs to the helicase family. DinG subfamily. Type 1 sub- subfamily. |
Keywords
[?]
| 4Fe-4S | |
| ATP-binding | |
| DNA-binding | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Hydrolase | |
| Iron | |
| Iron-sulfur | |
| Isomerase | |
| Metal-binding | |
| Nucleotide-binding | |
Gene Ontology
[?]
| GO:0043139; Molecular function:5'-3' DNA helicase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0003677; Molecular function:DNA binding |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
Cross-references
[?]
| PROSITE | PS51193; HELICASE_ATP_BIND_2; 1; |
| PROSITE | PS51194; HELICASE_CTER; 1; |
| Pfam | PF00270; DEAD; 1; |
| Pfam | PF06733; DEAD_2; 1; |
| Pfam | PF13307; Helicase_C_2; 1; |
Features
[?]
| From: DING_ECOLI (P27296) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 120 | 120 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 194 | 194 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 199 | 199 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 205 | 205 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
Additional information
[?]
| Size range | 650-780 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |