HAMAP rule MF_02211
General rule information
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| Accession | MF_02211 |
| Dates | 19-FEB-2019 (Created)
16-AUG-2023 (Last updated, Version 8) |
| Name | MpaA_carboxypeptidase |
| Scope(s) |
Bacteria Gammaproteobacteria |
| Template(s) | A7N805 (MPAA_VIBC1); P0ACV6 (MPAA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_02211 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MPAA |
| Protein name | RecName: Full=Murein peptide amidase A; EC=3.4.17.-; AltName: Full=Gamma-D-Glu-Dap amidase; AltName: Full=Zinc metallocarboxypeptidase MpaA; |
| Gene name | Name=mpaA; |
Comments
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| FUNCTION | Involved in muropeptide degradation. Catalyzes the hydrolysis of the gamma-D-glutamyl-diaminopimelic acid (gamma-D-Glu-Dap) amide bond in the murein tripeptide L-alanyl-gamma-D-glutamyl-meso- diaminopimelic acid, leading to the formation of L-Ala-gamma-D-Glu and Dap. |
| CATALYTIC ACTIVITY | Reaction=H2O + L-alanyl-gamma-D-glutamyl-meso-diaminoheptanedioate = L- alanyl-D-glutamate + meso-2,6-diaminoheptanedioate; Xref=Rhea:RHEA:28398, ChEBI:CHEBI:15377, ChEBI:CHEBI:57791, ChEBI:CHEBI:61395, ChEBI:CHEBI:61401; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| PATHWAY | Cell wall degradation; peptidoglycan degradation. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the peptidase M14 family. |
Keywords
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| Carboxypeptidase | |
| Cell wall biogenesis/degradation | |
| Cytoplasm | |
| Hydrolase | |
| Metalloprotease | |
| Protease | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0061473; Molecular function:murein tripeptide carboxypeptidase activity | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0009253; Biological process:peptidoglycan catabolic process | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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Features
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| From: MPAA_VIBC1 (A7N805) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 60 | 60 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
H | 1 | |||||||
| BINDING | 63 | 63 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
E | 1 | |||||||
| BINDING | 168 | 168 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
H | 1 | |||||||
Additional information
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| Size range | 220-280 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |