HAMAP rule MF_02213

General rule information [?]

Accession	MF_02213
Dates	7-MAR-2019 (Created) 14-MAY-2022 (Last updated, Version 5)

Name	Lipid_II_synth_GatD

Scope

Bacteria

Archaea

Templates

A0A0H2WZ38 (GATD_STAAC); A0A0H3JN63 (GATD_STAAN); Q8DNZ8 (GATD_STRR6): [Recover all]

Triggered by

HAMAP; MF_02213 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

GATD

Protein name

RecName:		Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD;
		EC 6.3.5.13;
AltName:		Full=Lipid II isoglutaminyl synthase glutaminase subunit;
		EC 3.5.1.2;

Gene name

gatD

Comments [?]

Function	The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity	RHEA:57928: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate EC 6.3.5.13
	RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+) EC 3.5.1.2
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit	Forms a heterodimer with MurT.
Similarity	Belongs to the CobB/CobQ family. GatD subfamily.

Keywords [?]

Cell shape
Cell wall biogenesis/degradation
Glutamine amidotransferase
Hydrolase
Ligase
Peptidoglycan synthesis

Gene Ontology [?]

GO:0140282; Molecular function: carbon-nitrogen ligase activity on lipid II.
GO:0004359; Molecular function: glutaminase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.

Cross-references [?]

PROSITE	PS51274; GATASE_COBBQ; 1; trigger=PRU00606;
Pfam	PF07685; GATase_3; 1;

Features [?]

From: GATD_STAAC (A0A0H2WZ38)

Key From To Description Tag Condition FTGroup

ACT_SITE 94 94 Nucleophile C

ACT_SITE 189 189 H

BINDING 128 128 /ligand="substrate R

Additional information [?]

Size range	210-390 amino acids
Related rules	None
Fusion	None

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