HAMAP rule MF_02213
General rule information
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| Accession | MF_02213 |
| Dates | 7-MAR-2019 (Created)
14-MAY-2022 (Last updated, Version 5) |
| Name | Lipid_II_synth_GatD |
| Scope(s) |
Bacteria Archaea |
| Template(s) | A0A0H2WZ38 (GATD_STAAC); A0A0H3JN63 (GATD_STAAN); Q8DNZ8 (GATD_STRR6); [ Recover all ] |
| Triggered by |
HAMAP; MF_02213 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GATD |
| Protein name | RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD; EC=6.3.5.13; AltName: Full=Lipid II isoglutaminyl synthase glutaminase subunit; EC=3.5.1.2; |
| Gene name | Name=gatD; |
Comments
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| FUNCTION | The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT. |
| CATALYTIC ACTIVITY | Reaction=ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys- D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L- glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D- isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:57928, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:60033, ChEBI:CHEBI:62233, ChEBI:CHEBI:456216; EC=6.3.5.13; |
| CATALYTIC ACTIVITY | Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2; |
| PATHWAY | Cell wall biogenesis; peptidoglycan biosynthesis. |
| SUBUNIT | Forms a heterodimer with MurT. |
| SIMILARITY | Belongs to the CobB/CobQ family. GatD subfamily. |
Keywords
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| Cell shape |
| Cell wall biogenesis/degradation |
| Glutamine amidotransferase |
| Hydrolase |
| Ligase |
| Peptidoglycan synthesis |
Gene Ontology
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| GO:0140282; Molecular function:carbon-nitrogen ligase activity on lipid II |
| GO:0004359; Molecular function:glutaminase activity |
| GO:0009252; Biological process:peptidoglycan biosynthetic process |
Cross-references
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Features
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| From: GATD_STAAC (A0A0H2WZ38) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 94 | 94 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 189 | 189 | H | |||||||||
| BINDING | 128 | 128 | /ligand="substrate" | R | ||||||||
Additional information
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| Size range | 210-390 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |