HAMAP rule MF_02213
General rule information
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Accession | MF_02213 |
Dates | 7-MAR-2019 (Created) 14-MAY-2022 (Last updated, Version 5) |
Name | Lipid_II_synth_GatD |
Scope | Bacteria
Archaea |
Templates | A0A0H2WZ38 (GATD_STAAC); A0A0H3JN63 (GATD_STAAN); Q8DNZ8 (GATD_STRR6): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT. |
Catalytic activity | RHEA:57928: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate
EC 6.3.5.13 |
RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+)
EC 3.5.1.2 |
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Pathway | Cell wall biogenesis; peptidoglycan biosynthesis. |
Subunit | Forms a heterodimer with MurT. |
Similarity | Belongs to the CobB/CobQ family. GatD subfamily. |
Keywords
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Cell shape
Cell wall biogenesis/degradation
Glutamine amidotransferase
Hydrolase
Ligase
Peptidoglycan synthesis
Cell wall biogenesis/degradation
Glutamine amidotransferase
Hydrolase
Ligase
Peptidoglycan synthesis
Gene Ontology
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GO:0140282; Molecular function: carbon-nitrogen ligase activity on lipid II.
GO:0004359; Molecular function: glutaminase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0004359; Molecular function: glutaminase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
Cross-references
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Features
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From: GATD_STAAC (A0A0H2WZ38) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 94 | 94 | Nucleophile | C | ||||||||
ACT_SITE | 189 | 189 | H | |||||||||
BINDING | 128 | 128 | /ligand="substrate | R |
Additional information
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Size range | 210-390 amino acids |
Related rules | None |
Fusion | None |