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Annotation rule MF_02213
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General rule information [?]

Accession MF_02213
Dates 7-MAR-2019 (Created)
6-NOV-2020 (Last updated, Version 4)
Name Lipid_II_synth_GatD
Scope
Bacteria
Archaea
Templates A0A0H2WZ38 (GATD_STAAC); A0A0H3JN63 (GATD_STAAN); Q8DNZ8 (GATD_STRR6): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GATD
Protein name
RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD;
EC 6.3.5.13;
AltName: Full=Lipid II isoglutaminyl synthase glutaminase subunit;
EC 3.5.1.2;
Gene name
gatD

Comments [?]

Function The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity RHEA:57928: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate
EC 6.3.5.13
RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+)
EC 3.5.1.2
Pathway Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit Forms a heterodimer with MurT.
Similarity Belongs to the CobB/CobQ family. GatD subfamily.

Keywords [?]

Cell shape
Cell wall biogenesis/degradation
Glutamine amidotransferase
Hydrolase
Ligase
Peptidoglycan synthesis

Gene Ontology [?]

GO:0140282; Molecular function: carbon-nitrogen ligase activity on lipid II.
GO:0004359; Molecular function: glutaminase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.

Cross-references [?]

PROSITE PS51274; GATASE_COBBQ; 1; trigger=PRU00606;
Pfam PF07685; GATase_3; 1;

Features [?]

From: GATD_STAAC (A0A0H2WZ38)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     94     94       Nucleophile     C  
ACT_SITE     189     189             H  
BINDING     128     128       Substrate     R  

Additional information [?]

Size range 210-390 amino acids
Related rules None
Fusion None


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