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HAMAP rule MF_02217

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General rule information [?]

Accession MF_02217
Dates 11-JUN-2019 (Created)
13-OCT-2023 (Last updated, Version 6)
Name TrmR_methyltr
Scope(s) Bacteria
Template(s) O32036 (TRMR_BACSU); [ Recover all ]
Triggered by HAMAP; MF_02217 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRMR
Protein name RecName: Full=tRNA 5-hydroxyuridine methyltransferase;
                 EC=2.1.1.-;
AltName: Full=ho5U methyltransferase;
Gene name Name=trmR;

Comments [?]

FUNCTIONCatalyzes the methylation of 5-hydroxyuridine (ho5U) to form 5-methoxyuridine (mo5U) at position 34 in tRNAs.
CATALYTIC ACTIVITY Reaction=5-hydroxyuridine(34) in tRNA + S-adenosyl-L-methionine = 5- methoxyuridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60524, Rhea:RHEA-COMP:13381, Rhea:RHEA-COMP:15591, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:136877, ChEBI:CHEBI:143860;
SUBUNITHomodimer.
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Keywords [?]


Gene Ontology [?]

GO:0016300; Molecular function:tRNA (uridine) methyltransferase activity
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0030488; Biological process:tRNA methylation

Cross-references [?]

PROSITE PS51682; SAM_OMT_I; 1;
Pfam PF01596; Methyltransf_3; 1;

Features [?]

From: TRMR_BACSU (O32036)
Key From To Description Tag Condition FTGroup
BINDING 113 114 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[DE]-A
BINDING 133 133 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 159 159 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 160 160 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
N 1
BINDING 38 38 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[MVIL]
BINDING 68 68 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
S
BINDING 85 85 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[ED]
BINDING 133 133 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
D

Additional information [?]

Size range 190-270 amino acids
Related rules None
Fusion Nter: None Cter: None



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