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HAMAP rule MF_02221

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General rule information [?]

Accession MF_02221
Dates 6-AUG-2019 (Created)
19-NOV-2022 (Last updated, Version 4)
Name CPSase
Archaea; Methanobacteria
Archaea; Methanopyri
Template A5UK38 (CPS_METS3)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Carbamoyl-phosphate synthase;
AltName: Full=Carbamoyl phosphate synthetase;

Comments [?]

Function Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate.
Catalytic activity RHEA:18029: 2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate
PhysiologicalDirection=left-to-right (RHEA:18030)
Cofactor Mg(2+)
Note: Binds 2 magnesium or manganese ions per subunit
Similarity Belongs to the small carbamoyl-phosphate synthase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004087; Molecular function: carbamoyl-phosphate synthase (ammonia) activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0030145; Molecular function: manganese ion binding.

Cross-references [?]

PROSITE PS50975; ATP_GRASP; 1; trigger=PRU00409;
Pfam PF02655; ATP-grasp_3; 1;

Features [?]

From: CPS_METS3 (A5UK38)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     137     186       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        

Additional information [?]

Size range 338-480 amino acids
Related rules None
Fusion None