HAMAP rule MF_02221
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02221 |
| Accession | MF_02221 |
| Dates | 6-AUG-2019 (Created)
2-SEP-2024 (Last updated, Version 5) |
| Name | CPSase |
| Scope(s) |
Archaea Methanobacteria Methanopyri |
| Template(s) | A5UK38 (CPS_METS3); [ Recover all ] |
| Triggered by |
HAMAP; MF_02221 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CPS |
| Protein name | RecName: Full=Carbamoyl-phosphate synthase; EC=6.3.4.16; AltName: Full=Carbamoyl phosphate synthetase; Short=CPSase; |
Comments
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| FUNCTION | Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate. |
| CATALYTIC ACTIVITY | Reaction=hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+); Xref=Rhea:RHEA:18029, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:456216; EC=6.3.4.16; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18030; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 magnesium or manganese ions per subunit |
| SIMILARITY | Belongs to the small carbamoyl-phosphate synthase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004087; Molecular function:carbamoyl-phosphate synthase (ammonia) activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0030145; Molecular function:manganese ion binding |
Cross-references
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Features
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| From: CPS_METS3 (A5UK38) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 137 | 186 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
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Additional information
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| Size range | 338-480 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |