HAMAP rule MF_02221

General rule information [?]

Accession	MF_02221
Dates	6-AUG-2019 (Created) 19-NOV-2022 (Last updated, Version 4)

Name

CPSase

Scope

Archaea; Methanobacteria

Archaea; Methanopyri

Template

A5UK38 (CPS_METS3)

Triggered by

HAMAP; MF_02221 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

CPS

Protein name

RecName:		Full=Carbamoyl-phosphate synthase;
		EC 6.3.4.16;
AltName:		Full=Carbamoyl phosphate synthetase;
		Short=CPSase;

Comments [?]

Function	Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate.
Catalytic activity	RHEA:18029: 2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate EC 6.3.4.16 PhysiologicalDirection=left-to-right (RHEA:18030)
Cofactor	Mg(2+) Mn(2+) Note: Binds 2 magnesium or manganese ions per subunit
Similarity	Belongs to the small carbamoyl-phosphate synthase family.

Keywords [?]

ATP-binding
Ligase
Magnesium
Manganese
Metal-binding
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004087; Molecular function: carbamoyl-phosphate synthase (ammonia) activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0030145; Molecular function: manganese ion binding.

Cross-references [?]

PROSITE	PS50975; ATP_GRASP; 1; trigger=PRU00409;
Pfam	PF02655; ATP-grasp_3; 1;

Features [?]

From: CPS_METS3 (A5UK38)

Key From To Description Tag Condition FTGroup

BINDING 137 186 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616

Additional information [?]

Size range	338-480 amino acids
Related rules	None
Fusion	None

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