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HAMAP rule MF_02225

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_02225
Accession MF_02225
Dates 23-AUG-2019 (Created)
02-SEP-2024 (Last updated, Version 8)
Name CoaBC
Scope(s) Bacteria
Archaea
Template(s) P0ABQ0; Q58323; Q5JF17; [ Recover all ]
Triggered by HAMAP; MF_02225 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier COABC
Protein name RecName: Full=Coenzyme A biosynthesis bifunctional protein CoaBC;
AltName: Full=DNA/pantothenate metabolism flavoprotein;
AltName: Full=Phosphopantothenoylcysteine synthetase/decarboxylase;
                 Short=PPCS-PPCDC;
                 Includes:
RecName: Full=Phosphopantothenoylcysteine decarboxylase;
                 Short=PPC decarboxylase;
                 Short=PPC-DC;
                 EC=4.1.1.36;
AltName: Full=CoaC;
                 Includes:
RecName: Full=Phosphopantothenate--cysteine ligase;
                 EC=6.3.2.5;
AltName: Full=CoaB;
AltName: Full=Phosphopantothenoylcysteine synthetase;
                 Short=PPC synthetase;
                 Short=PPC-S;
Gene name Name=coaBC;

Comments [?]

FUNCTIONCatalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'- phosphopantotheine.
CATALYTIC ACTIVITY Reaction=N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'- phosphopantetheine + CO2; Xref=Rhea:RHEA:16793, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:59458, ChEBI:CHEBI:61723; EC=4.1.1.36;
CATALYTIC ACTIVITY Reaction=(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4- phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+); Xref=Rhea:RHEA:19397, ChEBI:CHEBI:10986, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:37563, ChEBI:CHEBI:59458, ChEBI:CHEBI:60377; EC=6.3.2.5;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.;
case <OC:Bacteria>
PATHWAYCofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)- pantothenate: step 2/5.
PATHWAYCofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)- pantothenate: step 3/5.
else case <OC:Archaea>
PATHWAYCofactor biosynthesis; coenzyme A biosynthesis.
end case
SIMILARITYIn the N-terminal section; belongs to the HFCD (homo- oligomeric flavin containing Cys decarboxylase) superfamily.
SIMILARITYIn the C-terminal section; belongs to the PPC synthetase family.

Keywords [?]

Decarboxylase
Flavoprotein
FMN
Ligase
Lyase
Magnesium
Metal-binding
Multifunctional enzyme

Gene Ontology [?]

GO:0010181; Molecular function:FMN binding
GO:0004632; Molecular function:phosphopantothenate--cysteine ligase activity
GO:0004633; Molecular function:phosphopantothenoylcysteine decarboxylase activity
GO:0015937; Biological process:coenzyme A biosynthetic process

Cross-references [?]

Pfam PF04127; DFP; 1;
Pfam PF02441; Flavoprotein; 1;
NCBIfam TIGR00521; coaBC_dfp; 1;

Features [?]

From: COABC_ECOLI (P0ABQ0)
Key From To Description Tag Condition FTGroup
BINDING 273 275 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 G-C-A
BINDING 308 311 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 P-D-[ILVM]-[VLI]
REGION Nter 190 /note="Phosphopantothenoylcysteine decarboxylase"
REGION 191 Cter /note="Phosphopantothenate--cysteine ligase"
ACT_SITE 158 158 /note="Proton donor" C
BINDING 279 279 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 D
BINDING 289 289 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 K
BINDING 327 327 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 F
BINDING 341 341 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 K
BINDING 345 345 /ligand="CTP"
/ligand_id="ChEBI:CHEBI:37563"		 K

Additional information [?]

Size range 330-500 amino acids
Related rules None
Fusion Nter: None Cter: None



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