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HAMAP rule MF_02237

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General rule information [?]

Accession MF_02237
Dates 28-NOV-2019 (Created)
19-NOV-2022 (Last updated, Version 4)
Name NSUN6
Template O57712 (NSUN6_PYRHO)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=tRNA (cytosine(72)-C(5))-methyltransferase;
Short=tRNA:m(5)C72 MTase;
EC 2.1.1.-;

Comments [?]

Function S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs.
Catalytic activity RHEA:61988: cytidine(72) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(72) in tRNA + H(+) + S-adenosyl-L-homocysteine
PhysiologicalDirection=left-to-right (RHEA:61989)
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.

Keywords [?]

Gene Ontology [?]

GO:0016428; Molecular function: tRNA (cytosine-5-)-methyltransferase activity.
GO:0000049; Molecular function: tRNA binding.
GO:0006400; Biological process: tRNA modification.

Cross-references [?]

PROSITE PS50890; PUA; 0-1; trigger=PRU00161;
PS51686; SAM_MT_RSMB_NOP; 1;
Pfam PF01189; Methyltr_RsmB-F; 1;
PF01472; PUA; 0-1;
PF17125; Methyltr_RsmF_N; 0-1;
TIGRFAMs TIGR00446; nop2p; 0-1;
TIGR00451; unchar_dom_2; 0-1;

Features [?]

From: NSUN6_PYRHO (O57712)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     209     215       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [ACSV]-[ACPS]-[AGP]-[PQ]-[AG]-[GINS]-K  
ACT_SITE     327     327       Nucleophile     C  
BINDING (Optional)     233     233       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [DE]  
BINDING (Optional)     238     238       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [RK]  
BINDING (Optional)     260     260       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D  
BINDING (Optional)     277     277       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D  
BINDING (Optional)     304     304       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     Y  

Additional information [?]

Size range 320-420 amino acids
Related rules None
Fusion None