HAMAP rule MF_02241
General rule information
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| Accession | MF_02241 |
| Dates | 19-DEC-2019 (Created)
2-SEP-2024 (Last updated, Version 7) |
| Name | PIP_synthase |
| Scope(s) |
Bacteria |
| Template(s) | P9WPG7 (PIPS_MYCTU); A0A0H3MFZ3 (PIPS_MYCBP); Q9F7Y9 (PIPS_MYCS2); [ Recover all ] |
| Triggered by |
HAMAP; MF_02241 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PIPS |
| Protein name | RecName: Full=Phosphatidylinositol phosphate synthase; Short=PIP synthase; EC=2.7.8.-; AltName: Full=CDP-diacylglycerol--D-myo-inositol-3-phosphate 3-phosphatidyltransferase; |
Comments
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| FUNCTION | Catalyzes the conjugation of the 1'-hydroxyl group of D-myo- inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation. |
| CATALYTIC ACTIVITY | Reaction=a CDP-1,2-diacyl-sn-glycerol + 1D-myo-inositol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+); Xref=Rhea:RHEA:60504, ChEBI:CHEBI:15378, ChEBI:CHEBI:58088, ChEBI:CHEBI:58332, ChEBI:CHEBI:58401, ChEBI:CHEBI:60377; |
| CATALYTIC ACTIVITY | Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero- 3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+); Xref=Rhea:RHEA:61216, ChEBI:CHEBI:15378, ChEBI:CHEBI:58401, ChEBI:CHEBI:60377, ChEBI:CHEBI:85356, ChEBI:CHEBI:144472; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Contains a di-nuclear catalytic Mg(2+) center.; |
| PATHWAY | Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| SIMILARITY | Belongs to the CDP-alcohol phosphatidyltransferase class-I family. |
Keywords
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| Cell membrane |
| Lipid biosynthesis |
| Magnesium |
| Membrane |
| Metal-binding |
| Phospholipid biosynthesis |
| Phospholipid metabolism |
| Transferase |
| Transmembrane |
| Transmembrane helix |
Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0016780; Molecular function:phosphotransferase activity, for other substituted phosphate groups |
| GO:0008654; Biological process:phospholipid biosynthetic process |
| GO:0005886; Cellular component:plasma membrane |
Cross-references
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| PROSITE | PS00379; CDP_ALCOHOL_P_TRANSF; 1; |
| Pfam | PF01066; CDP-OH_P_transf; 1; |
| General | Transmembrane; -; 6; |
Features
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| From: PIPS_MYCTU (P9WPG7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 31 | 34 | /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332" |
[DN]-x-[VLIM]-T | ||||||||
| ACT_SITE | 93 | 93 | /note="Proton acceptor" | D | ||||||||
| BINDING | 68 | 68 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 68 | 68 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 71 | 71 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 89 | 89 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 89 | 89 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 93 | 93 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 72 | 72 | /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332" |
G | ||||||||
| BINDING | 76 | 76 | /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332" |
R | ||||||||
| BINDING | 82 | 82 | /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332" |
[TS] | ||||||||
Additional information
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| Size range | 190-290 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |