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HAMAP rule MF_02241

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General rule information [?]

Accession MF_02241
Dates 19-DEC-2019 (Created)
19-NOV-2022 (Last updated, Version 6)
Name PIP_synthase
Scope(s) Bacteria
Template(s) P9WPG7 (PIPS_MYCTU); A0A0H3MFZ3 (PIPS_MYCBP); Q9F7Y9 (PIPS_MYCS2); [ Recover all ]
Triggered by HAMAP; MF_02241 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PIPS
Protein name RecName: Full=Phosphatidylinositol phosphate synthase;
                 Short=PIP synthase;
                 EC=2.7.8.-;
AltName: Full=CDP-diacylglycerol--D-myo-inositol-3-phosphate 3-phosphatidyltransferase;

Comments [?]

FUNCTIONCatalyzes the conjugation of the 1'-hydroxyl group of D-myo- inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.
CATALYTIC ACTIVITY Reaction=1D-myo-inositol 3-phosphate + a CDP-1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+); Xref=Rhea:RHEA:60504, ChEBI:CHEBI:15378, ChEBI:CHEBI:58088, ChEBI:CHEBI:58332, ChEBI:CHEBI:58401, ChEBI:CHEBI:60377;
CATALYTIC ACTIVITY Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero- 3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+); Xref=Rhea:RHEA:61216, ChEBI:CHEBI:15378, ChEBI:CHEBI:58401, ChEBI:CHEBI:60377, ChEBI:CHEBI:85356, ChEBI:CHEBI:144472;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Contains a di-nuclear catalytic Mg(2+) center.;
PATHWAYPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCell membrane; Multi-pass membrane protein.
SIMILARITYBelongs to the CDP-alcohol phosphatidyltransferase class-I family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0016780; Molecular function:phosphotransferase activity, for other substituted phosphate groups
GO:0008654; Biological process:phospholipid biosynthetic process
GO:0005886; Cellular component:plasma membrane

Cross-references [?]

PROSITE PS00379; CDP_ALCOHOL_P_TRANSF; 1;
Pfam PF01066; CDP-OH_P_transf; 1;
General Transmembrane; -; 6;

Features [?]

From: PIPS_MYCTU (P9WPG7)
Key From To Description Tag Condition FTGroup
BINDING 31 34 /ligand="a CDP-1,2-diacyl-sn-glycerol"
/ligand_id="ChEBI:CHEBI:58332"
[DN]-x-[VLIM]-T
ACT_SITE 93 93 /note="Proton acceptor" D
BINDING 68 68 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
D
BINDING 68 68 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
D
BINDING 71 71 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
D
BINDING 89 89 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
D
BINDING 89 89 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
D
BINDING 93 93 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
D
BINDING 72 72 /ligand="a CDP-1,2-diacyl-sn-glycerol"
/ligand_id="ChEBI:CHEBI:58332"
G
BINDING 76 76 /ligand="a CDP-1,2-diacyl-sn-glycerol"
/ligand_id="ChEBI:CHEBI:58332"
R
BINDING 82 82 /ligand="a CDP-1,2-diacyl-sn-glycerol"
/ligand_id="ChEBI:CHEBI:58332"
[TS]

Additional information [?]

Size range 190-290 amino acids
Related rules None
Fusion Nter: None Cter: None



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