HAMAP rule MF_02241

General rule information [?]

Accession	MF_02241
Dates	19-DEC-2019 (Created) 19-NOV-2022 (Last updated, Version 6)

Name	PIP_synthase

Scope

Bacteria

Templates

P9WPG7 (PIPS_MYCTU); A0A0H3MFZ3 (PIPS_MYCBP); Q9F7Y9 (PIPS_MYCS2): [Recover all]

Triggered by

HAMAP; MF_02241 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PIPS

Protein name

RecName:		Full=Phosphatidylinositol phosphate synthase;
		Short=PIP synthase;
		EC 2.7.8.-;
AltName:		Full=CDP-diacylglycerol--D-myo-inositol-3-phosphate 3-phosphatidyltransferase;

Comments [?]

Function	Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.
Catalytic activity	RHEA:60504: 1D-myo-inositol 3-phosphate + a CDP-1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)
	RHEA:61216: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)
Cofactor	Mg(2+) Note: Contains a di-nuclear catalytic Mg(2+) center.
Pathway	Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Subunit	Homodimer.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Similarity	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Keywords [?]

Cell membrane
Lipid biosynthesis
Magnesium
Membrane
Metal-binding
Phospholipid biosynthesis
Phospholipid metabolism
Transferase
Transmembrane
Transmembrane helix

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0016780; Molecular function: phosphotransferase activity, for other substituted phosphate groups.
GO:0008654; Biological process: phospholipid biosynthetic process.
GO:0005886; Cellular component: plasma membrane.

Cross-references [?]

PROSITE	PS00379; CDP_ALCOHOL_P_TRANSF; 1;
Pfam	PF01066; CDP-OH_P_transf; 1;

Computed features [?]

General

Transmembrane; -; 6; trigger=yes;

Features [?]

From: PIPS_MYCTU (P9WPG7)

Key From To Description Tag Condition FTGroup

BINDING 31 34 /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332 [DN]-x-[VLIM]-T

ACT_SITE 93 93 Proton acceptor D

BINDING 68 68 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 68 68 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 71 71 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 89 89 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 89 89 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 93 93 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 72 72 /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332 G

BINDING 76 76 /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332 R

BINDING 82 82 /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332 [TS]

Additional information [?]

Size range	190-290 amino acids
Related rules	None
Fusion	None

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