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HAMAP rule MF_02241

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General rule information [?]

Accession MF_02241
Dates 19-DEC-2019 (Created)
19-NOV-2022 (Last updated, Version 6)
Name PIP_synthase
Templates P9WPG7 (PIPS_MYCTU); A0A0H3MFZ3 (PIPS_MYCBP); Q9F7Y9 (PIPS_MYCS2): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Phosphatidylinositol phosphate synthase;
Short=PIP synthase;
EC 2.7.8.-;
AltName: Full=CDP-diacylglycerol--D-myo-inositol-3-phosphate 3-phosphatidyltransferase;

Comments [?]

Function Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.
Catalytic activity RHEA:60504: 1D-myo-inositol 3-phosphate + a CDP-1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)
RHEA:61216: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)
Cofactor Mg(2+)
Note: Contains a di-nuclear catalytic Mg(2+) center.
Pathway Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Subunit Homodimer.
Subcellular location Cell membrane; Multi-pass membrane protein.
Similarity Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0016780; Molecular function: phosphotransferase activity, for other substituted phosphate groups.
GO:0008654; Biological process: phospholipid biosynthetic process.
GO:0005886; Cellular component: plasma membrane.

Cross-references [?]

Pfam PF01066; CDP-OH_P_transf; 1;

Computed features [?]

General Transmembrane; -; 6; trigger=yes;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     31     34       /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332     [DN]-x-[VLIM]-T  
ACT_SITE     93     93       Proton acceptor     D  
BINDING     68     68       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     68     68       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     71     71       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     89     89       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     89     89       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     93     93       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     72     72       /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332     G  
BINDING     76     76       /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332     R  
BINDING     82     82       /ligand="a CDP-1,2-diacyl-sn-glycerol" /ligand_id="ChEBI:CHEBI:58332     [TS]  

Additional information [?]

Size range 190-290 amino acids
Related rules None
Fusion None