HAMAP rule MF_02247
General rule information
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| Accession | MF_02247 |
| Dates | 1-MAR-2021 (Created)
1-JUN-2023 (Last updated, Version 5) |
| Name | Carbox_acid_reduct |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | B2HN69 (CAR_MYCMM); Q6RKB1 (CAR_NOCIO); E5XP76 (CAR_SEGRC); [ Recover all ] |
| Triggered by |
HAMAP; MF_02247 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CAR |
| Protein name | RecName: Full=Carboxylic acid reductase; Short=CAR; EC=1.2.1.-; AltName: Full=ATP/NADPH-dependent carboxylic acid reductase; |
| Gene name | Name=car; |
Comments
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| FUNCTION | Catalyzes the ATP- and NADPH-dependent reduction of carboxylic acids to the corresponding aldehydes. |
| CATALYTIC ACTIVITY | Reaction=a carboxylate + ATP + H(+) + NADPH = AMP + an aldehyde + diphosphate + NADP(+); Xref=Rhea:RHEA:50916, ChEBI:CHEBI:15378, ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:456215; |
| COFACTOR | Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; Note=Binds 1 phosphopantetheine covalently.; |
| DOMAIN | The N-terminal domain likely catalyzes substrate activation by formation of an initial acyl-AMP intermediate, the central region contains the phosphopantetheine attachment site, and the C-terminal domain catalyzes the reduction by NADPH of the intermediate thioester formed from the attack of the phosphopantetheine thiol at the carbonyl carbon of acyl-AMP. |
| SIMILARITY | Belongs to the ATP-dependent AMP-binding enzyme family. Carboxylic acid reductase subfamily. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0050661; Molecular function:NADP binding |
| GO:0016620; Molecular function:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| GO:0031177; Molecular function:phosphopantetheine binding |
Cross-references
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| PROSITE | PS00455; AMP_BINDING; 1; |
| PROSITE | PS50075; CARRIER; 1; |
| Pfam | PF00501; AMP-binding; 1; |
| Pfam | PF00550; PP-binding; 1; |
| Pfam | PF07993; NAD_binding_4; 1; |
| NCBIfam | TIGR01746; Thioester-redct; 1; |
Features
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| From: CAR_SEGRC (E5XP76) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 429 | 430 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
[DE]-G | ||||||||
| BINDING | 519 | 522 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
Y-[VL]-D-R | ||||||||
| BINDING | 801 | 804 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
[NT]-G-[FYW]-L | ||||||||
| BINDING | 868 | 869 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
D-[FK] | ||||||||
| BINDING | 894 | 896 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
[SP]-[GA]-A | ||||||||
| BINDING | 315 | 315 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
H | ||||||||
| BINDING | 408 | 408 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
[ST] | ||||||||
| BINDING | 434 | 434 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
T | ||||||||
| BINDING | 507 | 507 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
D | ||||||||
| BINDING | 528 | 528 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
K | ||||||||
| BINDING | 629 | 629 | /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215" |
K | ||||||||
| BINDING | 828 | 828 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
R | ||||||||
| BINDING | 838 | 838 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
R | ||||||||
| BINDING | 934 | 934 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
S | ||||||||
| BINDING | 970 | 970 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
Y | ||||||||
| BINDING | 974 | 974 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
K | ||||||||
| BINDING | 997 | 997 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
S | ||||||||
| MOD_RES | 702 | 702 | /note="O-(pantetheine 4'-phosphoryl)serine" | S | ||||||||
Additional information
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| Size range | 1000-1250 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |