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HAMAP rule MF_02247

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_02247
Accession MF_02247
Dates 01-MAR-2021 (Created)
02-SEP-2024 (Last updated, Version 7)
Name Carbox_acid_reduct
Scope(s) Bacteria
Actinomycetota
Template(s) B2HN69; Q6RKB1; E5XP76; [ Recover all ]
Triggered by HAMAP; MF_02247 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CAR
Protein name RecName: Full=Carboxylic acid reductase;
                 Short=CAR;
                 EC=1.2.1.-;
AltName: Full=ATP/NADPH-dependent carboxylic acid reductase;
Gene name Name=car;

Comments [?]

FUNCTIONCatalyzes the ATP- and NADPH-dependent reduction of carboxylic acids to the corresponding aldehydes.
CATALYTIC ACTIVITY Reaction=a carboxylate + ATP + NADPH + H(+) = an aldehyde + AMP + diphosphate + NADP(+); Xref=Rhea:RHEA:50916, ChEBI:CHEBI:15378, ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:456215;
COFACTOR Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; Note=Binds 1 phosphopantetheine covalently.;
DOMAINThe N-terminal domain likely catalyzes substrate activation by formation of an initial acyl-AMP intermediate, the central region contains the phosphopantetheine attachment site, and the C-terminal domain catalyzes the reduction by NADPH of the intermediate thioester formed from the attack of the phosphopantetheine thiol at the carbonyl carbon of acyl-AMP.
SIMILARITYBelongs to the ATP-dependent AMP-binding enzyme family. Carboxylic acid reductase subfamily.

Keywords [?]

ATP-binding
NADP
Nucleotide-binding
Oxidoreductase
Phosphopantetheine
Phosphoprotein

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0050661; Molecular function:NADP binding
GO:0016620; Molecular function:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0031177; Molecular function:phosphopantetheine binding

Cross-references [?]

PROSITE PS00455; AMP_BINDING; 1;
PROSITE PS50075; CARRIER; 1;
Pfam PF00501; AMP-binding; 1;
Pfam PF00550; PP-binding; 1;
Pfam PF07993; NAD_binding_4; 1;
NCBIfam TIGR01746; Thioester-redct; 1;

Features [?]

From: CAR_SEGRC (E5XP76)
Key From To Description Tag Condition FTGroup
BINDING 429 430 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 [DE]-G
BINDING 519 522 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 Y-[VL]-D-R
BINDING 801 804 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 [NT]-G-[FYW]-L
BINDING 868 869 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 D-[FK]
BINDING 894 896 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 [SP]-[GA]-A
BINDING 315 315 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 H
BINDING 408 408 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 [ST]
BINDING 434 434 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 T
BINDING 507 507 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 D
BINDING 528 528 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 K
BINDING 629 629 /ligand="AMP"
/ligand_id="ChEBI:CHEBI:456215"		 K
BINDING 828 828 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 R
BINDING 838 838 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 R
BINDING 934 934 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 S
BINDING 970 970 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 Y
BINDING 974 974 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 K
BINDING 997 997 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 S
MOD_RES 702 702 /note="O-(pantetheine 4'-phosphoryl)serine" S

Additional information [?]

Size range 1000-1250 amino acids
Related rules None
Fusion Nter: None Cter: None



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