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Annotation rule MF_02247
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General rule information [?]

Accession MF_02247
Dates 1-MAR-2021 (Created)
1-MAR-2021 (Last updated, Version 1)
Name Carbox_acid_reduct
Bacteria; Actinobacteria
Templates B2HN69 (CAR_MYCMM); Q6RKB1 (CAR_NOCIO); E5XP76 (CAR_SEGRC): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Carboxylic acid reductase;
EC 1.2.1.-;
AltName: Full=ATP/NADPH-dependent carboxylic acid reductase;
Gene name

Comments [?]

Function Catalyzes the ATP- and NADPH-dependent reduction of carboxylic acids to the corresponding aldehydes.
Catalytic activity RHEA:50916: a carboxylate + ATP + H(+) + NADPH = AMP + an aldehyde + diphosphate + NADP(+)
Cofactor pantetheine 4'-phosphate
Note: Binds 1 phosphopantetheine covalently.
Domain The N-terminal domain likely catalyzes substrate activation by formation of an initial acyl-AMP intermediate, the central region contains the phosphopantetheine attachment site, and the C-terminal domain catalyzes the reduction by NADPH of the intermediate thioester formed from the attack of the phosphopantetheine thiol at the carbonyl carbon of acyl-AMP.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. Carboxylic acid reductase subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0050661; Molecular function: NADP binding.
GO:0016620; Molecular function: oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor.
GO:0031177; Molecular function: phosphopantetheine binding.

Cross-references [?]

PS50075; CARRIER; 1; trigger=PRU00258;
Pfam PF00501; AMP-binding; 1;
PF00550; PP-binding; 1;
PF07993; NAD_binding_4; 1;
TIGRFAMs TIGR01746; Thioester-redct; 1;

Features [?]

From: CAR_SEGRC (E5XP76)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     429     430       AMP     [DE]-G  
NP_BIND (Optional)     519     522       AMP     Y-[VL]-D-R  
NP_BIND (Optional)     801     804       NADP     [NT]-G-[FYW]-L  
NP_BIND (Optional)     868     869       NADP     D-[FK]  
NP_BIND (Optional)     894     896       NADP     [SP]-[GA]-A  
BINDING (Optional)     315     315       AMP     H  
BINDING (Optional)     408     408       AMP     [ST]  
BINDING (Optional)     434     434       AMP     T  
BINDING (Optional)     507     507       AMP     D  
BINDING (Optional)     528     528       AMP     K  
BINDING (Optional)     629     629       AMP     K  
BINDING (Optional)     828     828       NADP     R  
BINDING (Optional)     838     838       NADP     R  
BINDING (Optional)     934     934       NADP; via carbonyl oxygen     S  
BINDING (Optional)     970     970       NADP     Y  
BINDING (Optional)     974     974       NADP     K  
BINDING (Optional)     997     997       NADP     S  
MOD_RES     702     702       O-(pantetheine 4'-phosphoryl)serine     S  

Additional information [?]

Size range 1000-1250 amino acids
Related rules None
Fusion None