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Annotation rule MF_03000
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General rule information [?]

Accession MF_03000
Dates 12-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 17)
Name eIF3a
Scope
Eukaryota
Templates Q14152 (EIF3A_HUMAN); P23116 (EIF3A_MOUSE); P34339 (EIF3A_CAEEL); Q9VN25 (EIF3A_DROME); O74760 (EIF3A_SCHPO); P38249 (EIF3A_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
EIF3A
case <OC:Vertebrata>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit A;
Short=eIF3a;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 10;
AltName: Full=eIF-3-theta;
else case <OC:Caenorhabditis>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit A;
Short=eIF3a;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 10;
AltName: Full=Egg-laying defective protein 45;
else case <OC:Fungi>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit A;
Short=eIF3a;
AltName: Full=Eukaryotic translation initiation factor 3 110 kDa subunit homolog;
Short=eIF3 p110;
AltName: Full=Translation initiation factor eIF3, p110 subunit homolog;
else
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit A;
Short=eIF3a;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 10;
end case
case <OC:Vertebrata>
Gene name
EIF3A, EIF3S10
else case <OC:Caenorhabditis>
Gene name
egl-45, eif-3.A
else case <OC:Drosophilidae>
Gene name
eIF3a, eIF3-S10
else case <OC:Fungi> and not <OC:Saccharomyces>
Gene name
TIF32
else case <OC:Saccharomyces>
Gene name
RPG1
end case

Comments [?]

case <OC:Mammalia>
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
else
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
end case
case <OC:Mammalia>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with EIF4G1. Also interacts with KRT7 and PIWIL2.
else case <OC:Vertebrata> and not <OC:Mammalia>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M).
else case <OC:Drosophilidae>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
else
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.
end case
Subcellular location Cytoplasm.
case <OC:Mammalia>
Ptm Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
end case
Similarity Belongs to the eIF-3 subunit A family.

Keywords [?]

case <OC:Mammalia>
end case

Gene Ontology [?]

GO:0003743; Molecular function: translation initiation factor activity.
GO:0003723; Molecular function: RNA binding.
GO:0001732; Biological process: formation of cytoplasmic translation initiation complex.
GO:0002183; Biological process: cytoplasmic translational initiation.
GO:0005737; Cellular component: cytoplasm.
GO:0005852; Cellular component: eukaryotic translation initiation factor 3 complex.
GO:0016282; Cellular component: eukaryotic 43S preinitiation complex.
GO:0033290; Cellular component: eukaryotic 48S preinitiation complex.

Cross-references [?]

Pfam PF01399; PCI; 1;
PROSITE PS50250; PCI; 1; trigger=PRU01185;
PS50315; GLY_RICH; 0-unlimited;
PS50312; ASP_RICH; 0-unlimited;
PS50313; GLU_RICH; 0-unlimited;
PS50318; LYS_RICH; 0-unlimited;
PS50323; ARG_RICH; 0-unlimited;

Computed features [?]

General Coiled_coil; -; 0-unlimited;

Features [?]

case <OC:Vertebrata>
From: EIF3A_HUMAN (Q14152)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     1     1       Removed     M  
end case
case <OC:Mammalia>
REGION     664     835       Interaction with EIF3B        
MOD_RES     881     881       Phosphoserine     S  
MOD_RES     1198     1198       Phosphoserine     S  
MOD_RES     1336     1336       Phosphoserine     S  
MOD_RES     1364     1364       Phosphoserine     S  
end case

Additional information [?]

Size range 826-1424 amino acids
Related rules None
Fusion None