HAMAP rule MF_03000
General rule information
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Accession | MF_03000 |
Dates | 12-FEB-2009 (Created) 20-NOV-2019 (Last updated, Version 17) |
Name | eIF3a |
Scope | Eukaryota |
Templates | Q14152 (EIF3A_HUMAN); P23116 (EIF3A_MOUSE); P34339 (EIF3A_CAEEL); Q9VN25 (EIF3A_DROME); O74760 (EIF3A_SCHPO); P38249 (EIF3A_YEAST): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Vertebrata>
Protein name |
|
else case <OC:Caenorhabditis>
Protein name |
|
else case <OC:Fungi>
Protein name |
|
else
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
else case <OC:Drosophilidae>
Gene name |
|
else case <OC:Fungi> and not <OC:Saccharomyces>
Gene name |
|
else case <OC:Saccharomyces>
Gene name |
|
end case
Comments
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case <OC:Mammalia>
Function | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. |
else
Function | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. |
end case
case <OC:Mammalia>
Subunit | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with EIF4G1. Also interacts with KRT7 and PIWIL2. |
else case <OC:Vertebrata> and not <OC:Mammalia>
Subunit | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M). |
else case <OC:Drosophilidae>
Subunit | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. |
else
Subunit | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. |
end case
Subcellular location | Cytoplasm. |
case <OC:Mammalia>
Ptm | Phosphorylated. Phosphorylation is enhanced upon serum stimulation. |
end case
Similarity | Belongs to the eIF-3 subunit A family. |
Keywords
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case <OC:Mammalia>
end case
Gene Ontology
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GO:0003743; Molecular function: translation initiation factor activity.
GO:0003723; Molecular function: RNA binding.
GO:0001732; Biological process: formation of cytoplasmic translation initiation complex.
GO:0002183; Biological process: cytoplasmic translational initiation.
GO:0005737; Cellular component: cytoplasm.
GO:0005852; Cellular component: eukaryotic translation initiation factor 3 complex.
GO:0016282; Cellular component: eukaryotic 43S preinitiation complex.
GO:0033290; Cellular component: eukaryotic 48S preinitiation complex.
GO:0003723; Molecular function: RNA binding.
GO:0001732; Biological process: formation of cytoplasmic translation initiation complex.
GO:0002183; Biological process: cytoplasmic translational initiation.
GO:0005737; Cellular component: cytoplasm.
GO:0005852; Cellular component: eukaryotic translation initiation factor 3 complex.
GO:0016282; Cellular component: eukaryotic 43S preinitiation complex.
GO:0033290; Cellular component: eukaryotic 48S preinitiation complex.
Cross-references
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Pfam | PF01399; PCI; 1; |
PROSITE | PS50250; PCI; 1; trigger=PRU01185; |
PS50315; GLY_RICH; 0-unlimited; | |
PS50312; ASP_RICH; 0-unlimited; | |
PS50313; GLU_RICH; 0-unlimited; | |
PS50318; LYS_RICH; 0-unlimited; | |
PS50323; ARG_RICH; 0-unlimited; |
Computed features
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General | Coiled_coil; -; 0-unlimited; |
Features
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case <OC:Vertebrata>
From: EIF3A_HUMAN (Q14152) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
INIT_MET | 1 | 1 | Removed | M |
end case
case <OC:Mammalia>
REGION | 664 | 835 | Interaction with EIF3B | |||||||||
MOD_RES | 881 | 881 | Phosphoserine | S | ||||||||
MOD_RES | 1198 | 1198 | Phosphoserine | S | ||||||||
MOD_RES | 1336 | 1336 | Phosphoserine | S | ||||||||
MOD_RES | 1364 | 1364 | Phosphoserine | S |
end case
Additional information
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Size range | 826-1424 amino acids |
Related rules | None |
Fusion | None |