HAMAP rule MF_03000

Accession	MF_03000
Dates	12-FEB-2009 (Created) 20-NOV-2019 (Last updated, Version 17)

Name

eIF3a

Scope

Eukaryota

Templates

Q14152 (EIF3A_HUMAN); P23116 (EIF3A_MOUSE); P34339 (EIF3A_CAEEL); Q9VN25 (EIF3A_DROME); O74760 (EIF3A_SCHPO); P38249 (EIF3A_YEAST): [Recover all]

Triggered by

HAMAP; MF_03000 (Get profile general information and statistics)

Identifier

EIF3A

Protein name

RecName: Full=Eukaryotic translation initiation factor 3 subunit A; Short=eIF3a; AltName: Full=Eukaryotic translation initiation factor 3 subunit 10; AltName: Full=eIF-3-theta;

Protein name

RecName: Full=Eukaryotic translation initiation factor 3 subunit A; Short=eIF3a; AltName: Full=Eukaryotic translation initiation factor 3 subunit 10; AltName: Full=Egg-laying defective protein 45;

Protein name

RecName: Full=Eukaryotic translation initiation factor 3 subunit A; Short=eIF3a; AltName: Full=Eukaryotic translation initiation factor 3 110 kDa subunit homolog; Short=eIF3 p110; AltName: Full=Translation initiation factor eIF3, p110 subunit homolog;

Protein name

RecName: Full=Eukaryotic translation initiation factor 3 subunit A; Short=eIF3a; AltName: Full=Eukaryotic translation initiation factor 3 subunit 10;

Gene name

EIF3A, EIF3S10

Gene name

egl-45, eif-3.A

Gene name

eIF3a, eIF3-S10

Gene name

TIF32

Gene name

RPG1

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.

Subunit

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with EIF4G1. Also interacts with KRT7 and PIWIL2.

Subunit

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M).

Subunit

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

Subunit

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.

Subcellular location

Cytoplasm.

Ptm	Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Similarity

Belongs to the eIF-3 subunit A family.

Cytoplasm
Initiation factor
RNA-binding

Phosphoprotein

Protein biosynthesis

GO:0003743; Molecular function: translation initiation factor activity.
GO:0003723; Molecular function: RNA binding.
GO:0001732; Biological process: formation of cytoplasmic translation initiation complex.
GO:0002183; Biological process: cytoplasmic translational initiation.
GO:0005737; Cellular component: cytoplasm.
GO:0005852; Cellular component: eukaryotic translation initiation factor 3 complex.
GO:0016282; Cellular component: eukaryotic 43S preinitiation complex.
GO:0033290; Cellular component: eukaryotic 48S preinitiation complex.

Pfam	PF01399; PCI; 1;
PROSITE	PS50250; PCI; 1; trigger=PRU01185;
	PS50315; GLY_RICH; 0-unlimited;
	PS50312; ASP_RICH; 0-unlimited;
	PS50313; GLU_RICH; 0-unlimited;
	PS50318; LYS_RICH; 0-unlimited;
	PS50323; ARG_RICH; 0-unlimited;

General

Coiled_coil; -; 0-unlimited;

From: EIF3A_HUMAN (Q14152)

Key

From

To

Description

Tag

Condition

FTGroup

INIT_MET

1

1

Removed

M

REGION		664		835		Interaction with EIF3B
MOD_RES		881		881		Phosphoserine				S
MOD_RES		1198		1198		Phosphoserine				S
MOD_RES		1336		1336		Phosphoserine				S
MOD_RES		1364		1364		Phosphoserine				S

Size range	826-1424 amino acids
Related rules	None
Fusion	None

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