HAMAP rule MF_03014
General rule information
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| Accession | MF_03014 |
| Dates | 12-FEB-2009 (Created)
23-MAY-2024 (Last updated, Version 11) |
| Name | KFase |
| Scope(s) |
Eukaryota |
| Template(s) | Q9VMC9 (KFA_DROME); Q8K4H1 (KFA_MOUSE); Q04066 (KFA_YEAST); [ Recover all ] |
| Triggered by |
HAMAP; MF_03014 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | KFA |
| Protein name | RecName: Full=Kynurenine formamidase; Short=KFase; Short=KFA; EC=3.5.1.9; AltName: Full=Arylformamidase; AltName: Full=N-formylkynurenine formamidase; Short=FKF; |
| case <OC:Vertebrata> | |
| Gene name | Name=AFMID; |
| else case <OC:Saccharomycotina> | |
| Gene name | Name=BNA7; |
| end case | |
Comments
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| case <OC:Vertebrata> or <OC:Fungi> | |
| FUNCTION | Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. |
| else | |
| FUNCTION | Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Required for elimination of toxic metabolites. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=H2O + N-formyl-L-kynurenine = formate + H(+) + L-kynurenine; Xref=Rhea:RHEA:13009, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57959, ChEBI:CHEBI:58629; EC=3.5.1.9; |
| PATHWAY | Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2. |
| case <OC:Mammalia> | |
| SUBCELLULAR LOCATION | Cytoplasm, cytosol. Nucleus. Note=Predominantly cytosolic. Some fraction is nuclear. |
| else case <OC:Vertebrata> and not <OC:Mammalia> | |
| SUBCELLULAR LOCATION | Cytoplasm, cytosol. Nucleus. |
| end case | |
| SUBUNIT | Homodimer. |
| case <FTTag:HGGXW> | |
| DOMAIN | The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage. |
| end case | |
| SIMILARITY | Belongs to the kynurenine formamidase family. |
Keywords
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| case <OC:Vertebrata> | |
| Cytoplasm | |
| Nucleus | |
| end case | |
| Hydrolase | |
| Tryptophan catabolism | |
Gene Ontology
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| GO:0004061; Molecular function:arylformamidase activity | |
| GO:0019441; Biological process:tryptophan catabolic process to kynurenine | |
| case <OCellular component:Vertebrata> or <OC:Fungi> | |
| GO:0034354; Biological process:'de novo' NAD biosynthetic process from tryptophan | |
| end case | |
| case <OCellular component:Vertebrata> | |
| GO:0005634; Cellular component:nucleus | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Cross-references
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Features
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| From: KFA_DROME (Q9VMC9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 86 | 90 | /note="HGGXW" | HGGXW | H-G-G-x-W | |||||||
| ACT_SITE | 157 | 157 | /note="Nucleophile" | S | ||||||||
| ACT_SITE | 244 | 244 | [DE] | |||||||||
| ACT_SITE | 276 | 276 | H | |||||||||
Additional information
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| Size range | 241-399 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |