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HAMAP rule MF_03014

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General rule information [?]

Accession MF_03014
Dates 12-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 10)
Name KFase
Scope
Eukaryota
Templates Q9VMC9 (KFA_DROME); Q8K4H1 (KFA_MOUSE); Q04066 (KFA_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
KFA
Protein name
RecName: Full=Kynurenine formamidase;
Short=KFase;
Short=KFA;
EC 3.5.1.9;
AltName: Full=Arylformamidase;
AltName: Full=N-formylkynurenine formamidase;
Short=FKF;
case <OC:Vertebrata>
Gene name
AFMID
else case <OC:Saccharomycotina>
Gene name
BNA7
end case

Comments [?]

case <OC:Vertebrata> or <OC:Fungi>
Function Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
else
Function Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Required for elimination of toxic metabolites.
end case
Catalytic activity RHEA:13009: H2O + N-formyl-L-kynurenine = formate + H(+) + L-kynurenine
EC 3.5.1.9
Pathway Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
case <OC:Mammalia>
Subcellular location Cytoplasm, cytosol. Nucleus. Note=Predominantly cytosolic. Some fraction is nuclear.
else case <OC:Vertebrata> and not <OC:Mammalia>
Subcellular location Cytoplasm, cytosol. Nucleus.
end case
Subunit Homodimer.
case <FTTag:HGGXW>
Domain The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.
end case
Similarity Belongs to the kynurenine formamidase family.

Keywords [?]

case <OC:Vertebrata>
Cytoplasm
Nucleus
end case
Hydrolase
Tryptophan catabolism

Gene Ontology [?]

GO:0004061; Molecular function: arylformamidase activity.
GO:0006569; Biological process: tryptophan catabolic process.
case <OC:Vertebrata> or <OC:Fungi>
GO:0034354; Biological process: 'de novo' NAD biosynthetic process from tryptophan.
end case
case <OC:Vertebrata>
GO:0005634; Cellular component: nucleus.
GO:0005737; Cellular component: cytoplasm.
end case

Features [?]

From: KFA_DROME (Q9VMC9)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     86     90       HGGXW   HGGXW   H-G-G-x-W  
ACT_SITE     157     157       Nucleophile     S  
ACT_SITE     244     244             [DE]  
ACT_SITE     276     276             H  

Additional information [?]

Size range 241-399 amino acids
Related rules None
Fusion None


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