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HAMAP rule MF_03014

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General rule information [?]

Accession MF_03014
Dates 12-FEB-2009 (Created)
23-MAY-2024 (Last updated, Version 11)
Name KFase
Scope(s) Eukaryota
Template(s) Q9VMC9 (KFA_DROME); Q8K4H1 (KFA_MOUSE); Q04066 (KFA_YEAST); [ Recover all ]
Triggered by HAMAP; MF_03014 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KFA
Protein name RecName: Full=Kynurenine formamidase;
                 Short=KFase;
                 Short=KFA;
                 EC=3.5.1.9;
AltName: Full=Arylformamidase;
AltName: Full=N-formylkynurenine formamidase;
                 Short=FKF;
case <OC:Vertebrata>
Gene name Name=AFMID;
else case <OC:Saccharomycotina>
Gene name Name=BNA7;
end case

Comments [?]

case <OC:Vertebrata> or <OC:Fungi>
FUNCTIONCatalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
else
FUNCTIONCatalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Required for elimination of toxic metabolites.
end case
CATALYTIC ACTIVITY Reaction=H2O + N-formyl-L-kynurenine = formate + H(+) + L-kynurenine; Xref=Rhea:RHEA:13009, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57959, ChEBI:CHEBI:58629; EC=3.5.1.9;
PATHWAYAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
case <OC:Mammalia>
SUBCELLULAR LOCATIONCytoplasm, cytosol. Nucleus. Note=Predominantly cytosolic. Some fraction is nuclear.
else case <OC:Vertebrata> and not <OC:Mammalia>
SUBCELLULAR LOCATIONCytoplasm, cytosol. Nucleus.
end case
SUBUNITHomodimer.
case <FTTag:HGGXW>
DOMAINThe main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.
end case
SIMILARITYBelongs to the kynurenine formamidase family.

Keywords [?]

case <OC:Vertebrata>
Cytoplasm
Nucleus
end case
Hydrolase
Tryptophan catabolism

Gene Ontology [?]

GO:0004061; Molecular function:arylformamidase activity
GO:0019441; Biological process:tryptophan catabolic process to kynurenine
case <OCellular component:Vertebrata> or <OC:Fungi>
GO:0034354; Biological process:'de novo' NAD biosynthetic process from tryptophan
end case
case <OCellular component:Vertebrata>
GO:0005634; Cellular component:nucleus
GO:0005737; Cellular component:cytoplasm
end case

Cross-references [?]


Features [?]

From: KFA_DROME (Q9VMC9)
Key From To Description Tag Condition FTGroup
MOTIF 86 90 /note="HGGXW" HGGXW H-G-G-x-W
ACT_SITE 157 157 /note="Nucleophile" S
ACT_SITE 244 244 [DE]
ACT_SITE 276 276 H

Additional information [?]

Size range 241-399 amino acids
Related rules None
Fusion Nter: None Cter: None



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