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Annotation rule MF_03023
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General rule information [?]

Accession MF_03023
Dates 20-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 19)
Name Katanin_p60_A1
Scope
Eukaryota
Templates O75449 (KTNA1_HUMAN); O61577 (KTNA1_STRPU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
KTNA1
case <OC:Caenorhabditis>
Protein name
RecName: Full=Meiotic spindle formation protein mei-1;
EC 5.6.1.1;
AltName: Full=Katanin p60 ATPase-containing subunit A1;
Short=Katanin p60 subunit A1;
AltName: Full=p60 katanin;
Gene name
mei-1
else
Protein name
RecName: Full=Katanin p60 ATPase-containing subunit A1;
Short=Katanin p60 subunit A1;
EC 5.6.1.1;
AltName: Full=p60 katanin;
Gene name
KATNA1
end case

Comments [?]

case <OC:Mammalia>
Function Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
else case <OC:Caenorhabditis>
Function Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. This activity may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles.
else case <OC:Metazoa>
Function Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.
else
Function Severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays.
end case
Catalytic activity Reaction=n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.; EC=5.6.1.1;
case <OC:Caenorhabditis>
Activity regulation ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with mei-2.
else case <OC:Metazoa>
Activity regulation ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with @gn(KATNB1).
end case
case <OC:Mammalia>
Subunit Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42. Interacts with KATNB1 and KATNBL1. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches.
else case <OC:Caenorhabditis>
Subunit Interacts with mei-2, which may serve as a targeting subunit. Also interacts with mel-26, which targets mei-1 for ubiquitin mediated proteolysis.
else case <OC:Metazoa>
Subunit Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with @gn(KATNB1), which may serve as a targeting subunit.
end case
case <OC:Mammalia>
Subcellular location Cytoplasm. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle. Note=Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis.
else case <OC:Caenorhabditis>
Subcellular location Cytoplasm, cytoskeleton, spindle pole. Note=Localized to the spindle poles and condensed chromatin during female meiosis. This localization requires mei-2. Also localized to the polar body.
else case <OC:Metazoa>
Subcellular location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle. Note=Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with @gn(KATNB1).
else
Subcellular location Cytoplasm, cytoskeleton.
end case
case <OC:Mammalia>
Domain The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.
Ptm Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.
end case
Similarity Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.

Keywords [?]

ATP-binding
Cytoplasm
Cytoskeleton
Isomerase
Microtubule
Nucleotide-binding
case <OC:Metazoa>
Cell cycle
Cell division
end case
case <OC:Caenorhabditis>
Meiosis
else case <OC:Metazoa>
Mitosis
end case
case <OC:Mammalia>
Phosphoprotein
Ubl conjugation
end case

Gene Ontology [?]

GO:0008017; Molecular function: microtubule binding.
GO:0051013; Biological process: microtubule severing.
GO:0005737; Cellular component: cytoplasm.
case <OC:Metazoa>
GO:0005813; Cellular component: centrosome.
GO:0005819; Cellular component: spindle.
end case

Cross-references [?]

PROSITE PS00674; AAA; 1;
Pfam PF00004; AAA; 1;

Features [?]

From: KTNA1_HUMAN (O75449)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     249     256       ATP     G-P-P-G-T-G-K-T  
case <OC:Mammalia>
MOD_RES     42     42       Phosphoserine; by DYRK2     S  
MOD_RES     109     109       Phosphoserine; by DYRK2     S  
MOD_RES     133     133       Phosphothreonine; by DYRK2     T  
end case

Additional information [?]

Size range 464-681 amino acids
Related rules None
Fusion None


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