HAMAP rule MF_03023
General rule information
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| Accession | MF_03023 |
| Dates | 20-FEB-2009 (Created)
19-NOV-2022 (Last updated, Version 22) |
| Name | Katanin_p60_A1 |
| Scope(s) |
Eukaryota |
| Template(s) | O75449 (KTNA1_HUMAN); O61577 (KTNA1_STRPU); [ Recover all ] |
| Triggered by |
HAMAP; MF_03023 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | KTNA1 |
| case <OC:Caenorhabditis> | |
| Protein name | RecName: Full=Meiotic spindle formation protein mei-1; EC=5.6.1.1; AltName: Full=Katanin p60 ATPase-containing subunit A1; Short=Katanin p60 subunit A1; AltName: Full=p60 katanin; |
| Gene name | Name=mei-1; |
| else | |
| Protein name | RecName: Full=Katanin p60 ATPase-containing subunit A1; Short=Katanin p60 subunit A1; EC=5.6.1.1; AltName: Full=p60 katanin; |
| Gene name | Name=KATNA1; |
| end case | |
Comments
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| case <OC:Mammalia> | |
| FUNCTION | Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth. |
| else case <OC:Caenorhabditis> | |
| FUNCTION | Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. This activity may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles. |
| else case <OC:Metazoa> | |
| FUNCTION | Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. |
| else | |
| FUNCTION | Severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.; EC=5.6.1.1; |
| case <OC:Caenorhabditis> | |
| ACTIVITY REGULATION | ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with mei-2. |
| else case <OC:Metazoa> | |
| ACTIVITY REGULATION | ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with @gn(KATNB1). |
| end case | |
| case <OC:Mammalia> | |
| SUBUNIT | Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42. Interacts with KATNB1 and KATNBL1. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches. |
| else case <OC:Caenorhabditis> | |
| SUBUNIT | Interacts with mei-2, which may serve as a targeting subunit. Also interacts with mel-26, which targets mei-1 for ubiquitin mediated proteolysis. |
| else case <OC:Metazoa> | |
| SUBUNIT | Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with @gn(KATNB1), which may serve as a targeting subunit. |
| end case | |
| case <OC:Mammalia> | |
| SUBCELLULAR LOCATION | Cytoplasm. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle. Note=Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis. |
| else case <OC:Caenorhabditis> | |
| SUBCELLULAR LOCATION | Cytoplasm, cytoskeleton, spindle pole. Note=Localized to the spindle poles and condensed chromatin during female meiosis. This localization requires mei-2. Also localized to the polar body. |
| else case <OC:Metazoa> | |
| SUBCELLULAR LOCATION | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle. Note=Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with @gn(KATNB1). |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm, cytoskeleton. |
| end case | |
| case <OC:Mammalia> | |
| DOMAIN | The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization. |
| PTM | Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation. |
| PTM | Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation. |
| end case | |
| SIMILARITY | Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily. |
Keywords
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| ATP-binding | |
| Cytoplasm | |
| Cytoskeleton | |
| Isomerase | |
| Microtubule | |
| Nucleotide-binding | |
| case <OC:Metazoa> | |
| Cell cycle | |
| Cell division | |
| end case | |
| case <OC:Caenorhabditis> | |
| Meiosis | |
| else case <OC:Metazoa> | |
| Mitosis | |
| end case | |
| case <OC:Mammalia> | |
| Phosphoprotein | |
| Ubl conjugation | |
| end case | |
Gene Ontology
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| GO:0008017; Molecular function:microtubule binding | |
| GO:0051013; Biological process:microtubule severing | |
| GO:0005737; Cellular component:cytoplasm | |
| case <OCellular component:Metazoa> | |
| GO:0005813; Cellular component:centrosome | |
| GO:0005819; Cellular component:spindle | |
| end case | |
Cross-references
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Features
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| From: KTNA1_HUMAN (O75449) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 249 | 256 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-P-P-G-T-G-K-T | ||||||||
| case <OC:Mammalia> | ||||||||||||
| MOD_RES | 42 | 42 | /note="Phosphoserine; by DYRK2" | S | ||||||||
| MOD_RES | 109 | 109 | /note="Phosphoserine; by DYRK2" | S | ||||||||
| MOD_RES | 133 | 133 | /note="Phosphothreonine; by DYRK2" | T | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 464-681 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |