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| Annotation rule MF_03048 |
General rule information
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| Accession | MF_03048 |
| Dates | 26-FEB-2009 (Created) 20-NOV-2019 (Last updated, Version 7) |
| Name | Urm1 |
| Scope | Eukaryota |
| Templates | P40554 (URM1_YEAST); Q9BTM9 (URM1_HUMAN): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
|
case <OC:Fungi> or <OC:Vertebrata>
| Protein name |
|
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else
| Protein name |
|
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end case
case <OC:Fungi> or <OC:Vertebrata>
| Gene name |
|
else case <OC:Caenorhabditis>
| Gene name |
|
else case <OC:Drosophilidae>
| Gene name |
|
end case
Comments
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case <OC:Fungi>
| Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog @gn(UBA4). The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as @gn(AHP1). The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. |
else case <OC:Mammalia>
| Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. |
else case <OC:Vertebrata> or <OC:Viridiplantae>
| Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. |
else
| Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4 homolog. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. |
end case
| Pathway | tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. |
case <OC:Mammalia>
| Subunit | Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. |
else case <OC:Saccharomyces>
| Subunit | Homodimer; homodimerization may provide an autoprotection to the highly active C-terminal residue before attacking its substrates. Interacts with NCS2 and NCS6. Forms a conjugate with the target protein AHP1. |
end case
case <OC:Saccharomyces>
| Subcellular location | Cytoplasm. Nucleus. |
else
| Subcellular location | Cytoplasm. |
end case
case <OC:Fungi>
| Ptm | C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of @gn(UBA4), then thiocarboxylated (-COSH) via the rhodanese domain of @gn(UBA4). |
else case <OC:Vertebrata>
| Ptm | C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3. |
else
| Ptm | C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog. |
end case
| Similarity | Belongs to the URM1 family. |
Keywords
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case <OC:Saccharomyces>
end case
Gene Ontology
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GO:0005829; Cellular component: cytosol.
case <OC:Saccharomyces>
GO:0005634; Cellular component: nucleus.
end case
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0032447; Biological process: protein urmylation.
GO:0034227; Biological process: tRNA thio-modification.
GO:0032447; Biological process: protein urmylation.
GO:0034227; Biological process: tRNA thio-modification.
Cross-references
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| Pfam | PF09138; Urm1; 1; |
Features
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| From: URM1_YEAST (P40554) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOD_RES | 99 | 99 | 1-thioglycine | G | ||||||||
| CROSSLNK | 99 | 99 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) | G | ||||||||
Additional information
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| Size range | 91-142 amino acids |
| Related rules | None |
| Fusion | None |