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Annotation rule MF_03048
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General rule information [?]

Accession MF_03048
Dates 26-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 7)
Name Urm1
Scope
Eukaryota
Templates P40554 (URM1_YEAST); Q9BTM9 (URM1_HUMAN): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
URM1
case <OC:Fungi> or <OC:Vertebrata>
Protein name
RecName: Full=Ubiquitin-related modifier 1;
else
Protein name
RecName: Full=Ubiquitin-related modifier 1 homolog;
end case
case <OC:Fungi> or <OC:Vertebrata>
Gene name
URM1
else case <OC:Caenorhabditis>
Gene name
urm-1
else case <OC:Drosophilidae>
Gene name
Urm1
end case

Comments [?]

case <OC:Fungi>
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog @gn(UBA4). The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as @gn(AHP1). The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Mammalia>
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Vertebrata> or <OC:Viridiplantae>
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4 homolog. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
end case
Pathway tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
case <OC:Mammalia>
Subunit Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
else case <OC:Saccharomyces>
Subunit Homodimer; homodimerization may provide an autoprotection to the highly active C-terminal residue before attacking its substrates. Interacts with NCS2 and NCS6. Forms a conjugate with the target protein AHP1.
end case
case <OC:Saccharomyces>
Subcellular location Cytoplasm. Nucleus.
else
Subcellular location Cytoplasm.
end case
case <OC:Fungi>
Ptm C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of @gn(UBA4), then thiocarboxylated (-COSH) via the rhodanese domain of @gn(UBA4).
else case <OC:Vertebrata>
Ptm C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
else
Ptm C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog.
end case
Similarity Belongs to the URM1 family.

Keywords [?]

case <OC:Saccharomyces>
end case

Gene Ontology [?]

GO:0005829; Cellular component: cytosol.
case <OC:Saccharomyces>
GO:0005634; Cellular component: nucleus.
end case
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0032447; Biological process: protein urmylation.
GO:0034227; Biological process: tRNA thio-modification.

Cross-references [?]

Pfam PF09138; Urm1; 1;

Features [?]

From: URM1_YEAST (P40554)
Key     From     To       Description   Tag   Condition   FTGroup
MOD_RES     99     99       1-thioglycine     G  
CROSSLNK     99     99       Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)     G  

Additional information [?]

Size range 91-142 amino acids
Related rules None
Fusion None