HAMAP rule MF_03048

General rule information [?]

Accession	MF_03048
Dates	26-FEB-2009 (Created) 20-NOV-2019 (Last updated, Version 7)
Name	Urm1
Scope(s)	Eukaryota
Template(s)	P40554 (URM1_YEAST); Q9BTM9 (URM1_HUMAN); [ Recover all ]
Triggered by	HAMAP; MF_03048 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	URM1
case <OC:Fungi> or <OC:Vertebrata>
Protein name	RecName: Full=Ubiquitin-related modifier 1;
else
Protein name	RecName: Full=Ubiquitin-related modifier 1 homolog;
end case
case <OC:Fungi> or <OC:Vertebrata>
Gene name	Name=URM1;
else case <OC:Caenorhabditis>
Gene name	Name=urm-1;
else case <OC:Drosophilidae>
Gene name	Name=Urm1;
end case

Comments [?]

case <OC:Fungi>
FUNCTION	Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog @gn(UBA4). The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as @gn(AHP1). The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Mammalia>
FUNCTION	Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Vertebrata> or <OC:Viridiplantae>
FUNCTION	Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else
FUNCTION	Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4 homolog. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
end case
PATHWAY	tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
case <OC:Mammalia>
SUBUNIT	Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
else case <OC:Saccharomyces>
SUBUNIT	Homodimer; homodimerization may provide an autoprotection to the highly active C-terminal residue before attacking its substrates. Interacts with NCS2 and NCS6. Forms a conjugate with the target protein AHP1.
end case
case <OC:Saccharomyces>
SUBCELLULAR LOCATION	Cytoplasm. Nucleus.
else
SUBCELLULAR LOCATION	Cytoplasm.
end case
case <OC:Fungi>
PTM	C-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of @gn(UBA4), then thiocarboxylated (-COSH) via the rhodanese domain of @gn(UBA4).
else case <OC:Vertebrata>
PTM	C-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
else
PTM	C-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog.
end case
SIMILARITY	Belongs to the URM1 family.

Keywords [?]

Cytoplasm
case <OC:Saccharomyces>
Nucleus
end case
Isopeptide bond
tRNA processing
Ubl conjugation pathway

Gene Ontology [?]

GO:0005829; Cellular component:cytosol
case <OCellular component:Saccharomyces>
GO:0005634; Cellular component:nucleus
end case
GO:0002098; Biological process:tRNA wobble uridine modification
GO:0032447; Biological process:protein urmylation
GO:0034227; Biological process:tRNA thio-modification

Cross-references [?]

Pfam	PF09138; Urm1; 1;

Features [?]

From: URM1_YEAST (P40554)

Key

From

Description

Tag

Condition

FTGroup

MOD_RES

/note="1-thioglycine"

CROSSLNK

/note="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)"

Additional information [?]

Size range	91-142 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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