HAMAP rule MF_03049

General rule information [?]

Accession	MF_03049
Dates	26-FEB-2009 (Created) 19-NOV-2022 (Last updated, Version 16)

Name	MOCS3_Uba4

Scope

Eukaryota

Templates

O95396 (MOCS3_HUMAN); P38820 (UBA4_YEAST); O59954 (UBA4_EMENI): [Recover all]

Triggered by

HAMAP; MF_03049 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Vertebrata> or <OC:Viridiplantae>

Identifier

MOCS3

Protein name

RecName:		Full=Adenylyltransferase and sulfurtransferase MOCS3;
AltName:		Full=Molybdenum cofactor synthesis protein 3;
Includes:
	RecName:		Full=Molybdopterin-synthase adenylyltransferase;
			EC 2.7.7.80;
	AltName:		Full=Adenylyltransferase MOCS3;
	AltName:		Full=Sulfur carrier protein MOCS2A adenylyltransferase;
Includes:
	RecName:		Full=Molybdopterin-synthase sulfurtransferase;
			EC 2.8.1.11;
	AltName:		Full=Sulfurtransferase MOCS3;
	AltName:		Full=Sulfur carrier protein MOCS2A sulfurtransferase;

else case <OC:Pezizomycotina>

Identifier

UBA4

Protein name

RecName:		Full=Adenylyltransferase and sulfurtransferase uba4;
AltName:		Full=Ubiquitin-like protein activator 4;
AltName:		Full=Common component for nitrate reductase and xanthine dehydrogenase protein F;
Includes:
	RecName:		Full=Molybdopterin-synthase adenylyltransferase;
			EC 2.7.7.80;
	AltName:		Full=Adenylyltransferase uba4;
	AltName:		Full=Sulfur carrier protein MOCS2A adenylyltransferase;
Includes:
	RecName:		Full=Molybdopterin-synthase sulfurtransferase;
			EC 2.8.1.11;
	AltName:		Full=Sulfurtransferase uba4;
	AltName:		Full=Sulfur carrier protein MOCS2A sulfurtransferase;

else case <OC:Fungi>

Identifier

UBA4

Protein name

RecName:		Full=Adenylyltransferase and sulfurtransferase ;
AltName:		Full=Ubiquitin-like protein activator 4;
Includes:
	RecName:		Full=Adenylyltransferase ;
			EC 2.7.7.-;
Includes:
	RecName:		Full=Sulfurtransferase ;
			EC 2.8.1.-;

else

Identifier

UBA4

Protein name

RecName:		Full=Adenylyltransferase and sulfurtransferase MOCS3 homolog;
AltName:		Full=Ubiquitin-like protein activator 4 homolog;
AltName:		Full=UBA4 homolog;
Includes:
	RecName:		Full=Adenylyltransferase;
			EC 2.7.7.-;
Includes:
	RecName:		Full=Sulfurtransferase;
			EC 2.8.1.-;

end case

case <OC:Vertebrata>

Gene name

MOCS3, UBA4

else case <OC:Caenorhabditis>

Gene name

moc-3, uba-4

else case <OC:Viridiplantae>

Gene name

MOCS3, CNX5, UBA4

else case <OC:Pezizomycotina>

Gene name

uba4, cnxF

else case <OC:Fungi>

Gene name

UBA4

else case <OC:Drosophilidae>

Gene name

Uba4

end case

Comments [?]

case <OC:Vertebrata> or <OC:Viridiplantae>

Function	Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers @gn(URM1) and MOCS2A. Its N-terminus first activates @gn(URM1) and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1) and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions.
Catalytic activity	RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate EC 2.7.7.80
	RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase] EC 2.8.1.11
Pathway	Cofactor biosynthesis; molybdopterin biosynthesis.

else case <OC:Pezizomycotina>

Function	Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.
Catalytic activity	RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate EC 2.7.7.80
	RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase] EC 2.8.1.11
Pathway	Cofactor biosynthesis; molybdopterin biosynthesis.

else case <OC:Fungi>

Function

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier @gn(URM1). Its N-terminus first activates @gn(URM1) as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1). Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.

else

Function

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

end case

Cofactor	Zn(2+) Note: Binds 1 zinc ion per subunit.
Pathway	tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

case <OC:Mammalia>

Subunit

Interacts with NFS1.

end case

Subcellular location	Cytoplasm.
Similarity	In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Keywords [?]

ATP-binding
Cytoplasm
Metal-binding
Multifunctional enzyme
Nucleotide-binding
Transferase
tRNA processing
Zinc

case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>

Molybdenum cofactor biosynthesis

else case <OC:Fungi>

Ubl conjugation pathway

end case

case <OC:Vertebrata>

Disulfide bond

end case

Gene Ontology [?]

GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.

case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>

GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.

else case <OC:Fungi>

GO:0032447; Biological process: protein urmylation.

end case

Cross-references [?]

PROSITE	PS50206; RHODANESE_3; 1; trigger=PRU00173;
Pfam	PF05237; MoeZ_MoeB; 1;
	PF00581; Rhodanese; 1;
	PF00899; ThiF; 1;

Features [?]

From: MOCS3_HUMAN (O95396)

Key From To Description Tag Condition FTGroup

BINDING 120 124 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 x-N-[LMF]-[HAQ]-R

BINDING 181 182 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [DN]-x

ACT_SITE 239 239 Glycyl thioester intermediate; for adenylyltransferase activity C

ACT_SITE 412 412 Cysteine persulfide intermediate; for sulfurtransferase activity C

BINDING 222 222 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 225 225 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 297 297 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 300 300 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 92 92 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G

BINDING 113 113 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 D

BINDING 137 137 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

case <OC:Vertebrata>

DISULFID 316 324 Alternate C-x*-C

end case

Additional information [?]

Size range	396-529 amino acids
Related rules	None
Fusion	None

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