HAMAP rule MF_03049
General rule information
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Accession | MF_03049 |
Dates | 26-FEB-2009 (Created) 19-NOV-2022 (Last updated, Version 16) |
Name | MOCS3_Uba4 |
Scope | Eukaryota |
Templates | O95396 (MOCS3_HUMAN); P38820 (UBA4_YEAST); O59954 (UBA4_EMENI): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Vertebrata> or <OC:Viridiplantae>
Identifier |
|
Protein name |
|
else case <OC:Pezizomycotina>
Identifier |
|
Protein name |
|
else case <OC:Fungi>
Identifier |
|
Protein name |
|
else
Identifier |
|
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
else case <OC:Viridiplantae>
Gene name |
|
else case <OC:Pezizomycotina>
Gene name |
|
else case <OC:Fungi>
Gene name |
|
else case <OC:Drosophilidae>
Gene name |
|
end case
Comments
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case <OC:Vertebrata> or <OC:Viridiplantae>
Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers @gn(URM1) and MOCS2A. Its N-terminus first activates @gn(URM1) and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1) and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions. |
Catalytic activity | RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
EC 2.7.7.80 |
RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]
EC 2.8.1.11 |
|
Pathway | Cofactor biosynthesis; molybdopterin biosynthesis. |
else case <OC:Pezizomycotina>
Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions. |
Catalytic activity | RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
EC 2.7.7.80 |
RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]
EC 2.8.1.11 |
|
Pathway | Cofactor biosynthesis; molybdopterin biosynthesis. |
else case <OC:Fungi>
Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier @gn(URM1). Its N-terminus first activates @gn(URM1) as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1). Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. |
else
Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
end case
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
Pathway | tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. |
case <OC:Mammalia>
Subunit | Interacts with NFS1. |
end case
Subcellular location | Cytoplasm. |
Similarity | In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily. |
Keywords
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ATP-binding
Cytoplasm
Metal-binding
Multifunctional enzyme
Nucleotide-binding
Transferase
tRNA processing
Zinc
Cytoplasm
Metal-binding
Multifunctional enzyme
Nucleotide-binding
Transferase
tRNA processing
Zinc
case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>
else case <OC:Fungi>
end case
case <OC:Vertebrata>
end case
Gene Ontology
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GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
else case <OC:Fungi>
GO:0032447; Biological process: protein urmylation.
end case
Cross-references
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PROSITE | PS50206; RHODANESE_3; 1; trigger=PRU00173; |
Pfam | PF05237; MoeZ_MoeB; 1; |
PF00581; Rhodanese; 1; | |
PF00899; ThiF; 1; |
Features
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From: MOCS3_HUMAN (O95396) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 120 | 124 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | x-N-[LMF]-[HAQ]-R | ||||||||
BINDING | 181 | 182 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | [DN]-x | ||||||||
ACT_SITE | 239 | 239 | Glycyl thioester intermediate; for adenylyltransferase activity | C | ||||||||
ACT_SITE | 412 | 412 | Cysteine persulfide intermediate; for sulfurtransferase activity | C | ||||||||
BINDING | 222 | 222 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 225 | 225 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 297 | 297 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 300 | 300 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 92 | 92 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G | ||||||||
BINDING | 113 | 113 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | D | ||||||||
BINDING | 137 | 137 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K |
case <OC:Vertebrata>
end case
Additional information
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Size range | 396-529 amino acids |
Related rules | None |
Fusion | None |