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Annotation rule MF_03049
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General rule information [?]

Accession MF_03049
Dates 26-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 13)
Name MOCS3_Uba4
Scope
Eukaryota
Templates O95396 (MOCS3_HUMAN); P38820 (UBA4_YEAST); O59954 (UBA4_EMENI): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Vertebrata> or <OC:Viridiplantae>
Identifier
MOCS3
Protein name
RecName: Full=Adenylyltransferase and sulfurtransferase MOCS3;
AltName: Full=Molybdenum cofactor synthesis protein 3;
Includes:
RecName: Full=Molybdopterin-synthase adenylyltransferase;
EC 2.7.7.80;
AltName: Full=Adenylyltransferase MOCS3;
AltName: Full=Sulfur carrier protein MOCS2A adenylyltransferase;
Includes:
RecName: Full=Molybdopterin-synthase sulfurtransferase;
EC 2.8.1.11;
AltName: Full=Sulfurtransferase MOCS3;
AltName: Full=Sulfur carrier protein MOCS2A sulfurtransferase;
else case <OC:Pezizomycotina>
Identifier
UBA4
Protein name
RecName: Full=Adenylyltransferase and sulfurtransferase uba4;
AltName: Full=Ubiquitin-like protein activator 4;
AltName: Full=Common component for nitrate reductase and xanthine dehydrogenase protein F;
Includes:
RecName: Full=Molybdopterin-synthase adenylyltransferase;
EC 2.7.7.80;
AltName: Full=Adenylyltransferase uba4;
AltName: Full=Sulfur carrier protein MOCS2A adenylyltransferase;
Includes:
RecName: Full=Molybdopterin-synthase sulfurtransferase;
EC 2.8.1.11;
AltName: Full=Sulfurtransferase uba4;
AltName: Full=Sulfur carrier protein MOCS2A sulfurtransferase;
else case <OC:Fungi>
Identifier
UBA4
Protein name
RecName: Full=Adenylyltransferase and sulfurtransferase ;
AltName: Full=Ubiquitin-like protein activator 4;
Includes:
RecName: Full=Adenylyltransferase ;
EC 2.7.7.-;
Includes:
RecName: Full=Sulfurtransferase ;
EC 2.8.1.-;
else
Identifier
UBA4
Protein name
RecName: Full=Adenylyltransferase and sulfurtransferase MOCS3 homolog;
AltName: Full=Ubiquitin-like protein activator 4 homolog;
AltName: Full=UBA4 homolog;
Includes:
RecName: Full=Adenylyltransferase;
EC 2.7.7.-;
Includes:
RecName: Full=Sulfurtransferase;
EC 2.8.1.-;
end case
case <OC:Vertebrata>
Gene name
MOCS3, UBA4
else case <OC:Caenorhabditis>
Gene name
moc-3, uba-4
else case <OC:Viridiplantae>
Gene name
MOCS3, CNX5, UBA4
else case <OC:Pezizomycotina>
Gene name
uba4, cnxF
else case <OC:Fungi>
Gene name
UBA4
else case <OC:Drosophilidae>
Gene name
Uba4
end case

Comments [?]

case <OC:Vertebrata> or <OC:Viridiplantae>
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers @gn(URM1) and MOCS2A. Its N-terminus first activates @gn(URM1) and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1) and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions.
Catalytic activity RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
EC 2.7.7.80
RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]
EC 2.8.1.11
Pathway Cofactor biosynthesis; molybdopterin biosynthesis.
else case <OC:Pezizomycotina>
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.
Catalytic activity RHEA:43616: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
EC 2.7.7.80
RHEA:48612: [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]
EC 2.8.1.11
Pathway Cofactor biosynthesis; molybdopterin biosynthesis.
else case <OC:Fungi>
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier @gn(URM1). Its N-terminus first activates @gn(URM1) as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to @gn(URM1) to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards @gn(URM1). Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; @gn(NFS1) probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
else
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
end case
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
Pathway tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
case <OC:Mammalia>
Subunit Interacts with NFS1.
end case
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Keywords [?]

case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>
else case <OC:Fungi>
end case
case <OC:Vertebrata>
end case

Gene Ontology [?]

GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0018192; Biological process: enzyme active site formation via cysteine modification to L-cysteine persulfide.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
case <OC:Metazoa> or <OC:Viridiplantae> or <OC:Pezizomycotina>
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
else case <OC:Fungi>
GO:0032447; Biological process: protein urmylation.
end case

Cross-references [?]

PROSITE PS50206; RHODANESE_3; 1; trigger=PRU00173;
Pfam PF05237; MoeZ_MoeB; 1;
PF00581; Rhodanese; 1;
PF00899; ThiF; 1;

Features [?]

From: MOCS3_HUMAN (O95396)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     120     124       ATP     x-N-[LMF]-[HAQ]-R  
NP_BIND     181     182       ATP     [DN]-x  
ACT_SITE     239     239       Glycyl thioester intermediate; for adenylyltransferase activity     C  
ACT_SITE     412     412       Cysteine persulfide intermediate; for sulfurtransferase activity     C  
METAL     222     222       Zinc     C  
METAL     225     225       Zinc     C  
METAL     297     297       Zinc     C  
METAL     300     300       Zinc     C  
BINDING     92     92       ATP; via amide nitrogen     G  
BINDING     113     113       ATP     D  
BINDING     137     137       ATP     K  
case <OC:Vertebrata>
DISULFID     316     324       Alternate     C-x*-C  
end case

Additional information [?]

Size range 396-529 amino acids
Related rules None
Fusion None