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Annotation rule MF_03055
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General rule information [?]

Accession MF_03055
Dates 13-MAR-2009 (Created)
20-NOV-2019 (Last updated, Version 7)
Name tRNA_methyltr_TrmB_euk
Scope
Eukaryota
Templates Q9UBP6 (TRMB_HUMAN); Q12009 (TRMB_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRMB
Protein name
RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
EC 2.1.1.33;
AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase;
AltName: Full=tRNA(m7G46)-methyltransferase;
case <OC:Vertebrata>
Protein name
Full=Methyltransferase-like protein 1;
else case <OC:Fungi>
Protein name
Full=Transfer RNA methyltransferase 8;
end case
case <OC:Vertebrata> or <OC:Dictyostelium>
Gene name
METTL1
else case <OC:Caenorhabditis>
Gene name
metl-1
else case <OC:Fungi>
Gene name
TRM8
end case

Comments [?]

case <OC:Saccharomyces>
Function Methyltransferase that catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity.
else
Function Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
end case
Catalytic activity RHEA:42708: guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine
EC 2.1.1.33
Pathway tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Vertebrata>
Subunit Forms a complex with @gn(WDR4).
else case <OC:Fungi>
Subunit Forms a complex with @gn(TRM82).
end case
Subcellular location Nucleus.
case <FTTag:phospho>
Ptm Phosphorylation at #{Ser-27} inactivates its catalytic activity but does not affect the interaction with @gn(WDR4).
end case
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]

case <FTTag:phospho>
end case

Gene Ontology [?]

GO:0000049; Molecular function: tRNA binding.
GO:0008176; Molecular function: tRNA (guanine-N7-)-methyltransferase activity.
GO:0005634; Cellular component: nucleus.
GO:0030488; Biological process: tRNA methylation.

Cross-references [?]

PROSITE PS51625; SAM_MT_TRMB; 1;
Pfam PF02390; Methyltransf_4; 1;
TIGRFAMs TIGR00091; CHP91; 1;

Features [?]

From: TRMB_HUMAN (Q9UBP6)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     107     108       S-adenosyl-L-methionine binding     E-[IL]  
REGION     140     141       S-adenosyl-L-methionine binding     N-x  
REGION     238     240       S-adenosyl-L-methionine binding     [ST]-[ED]-[ED]  
ACT_SITE     163     163             D  
BINDING     84     84       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     160     160       S-adenosyl-L-methionine; via carbonyl oxygen     [LC]  
case <OC:Mammalia>
MOD_RES     27     27       Phosphoserine; by PKB and RPS6KA3   phospho   S  
end case

Additional information [?]

Size range 214-377 amino acids
Related rules None
Fusion None