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HAMAP rule MF_03055

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General rule information [?]

Accession MF_03055
Dates 13-MAR-2009 (Created)
13-OCT-2023 (Last updated, Version 12)
Name tRNA_methyltr_TrmB_euk
Scope(s) Eukaryota
Template(s) Q9UBP6 (TRMB_HUMAN); Q12009 (TRMB_YEAST); [ Recover all ]
Triggered by HAMAP; MF_03055 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRMB
Protein name RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase;
AltName: Full=tRNA(m7G46)-methyltransferase;
case <OC:Vertebrata>
Protein name + AltName: Full=Methyltransferase-like protein 1;
else case <OC:Fungi>
Protein name + AltName: Full=Transfer RNA methyltransferase 8;
end case
case <OC:Vertebrata> or <OC:Dictyostelium>
Gene name Name=METTL1;
else case <OC:Caenorhabditis>
Gene name Name=metl-1;
else case <OC:Fungi>
Gene name Name=TRM8;
end case

Comments [?]

case <OC:Saccharomyces>
FUNCTIONMethyltransferase that catalyzes the formation of N(7)- methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity.
FUNCTIONCatalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
end case
CATALYTIC ACTIVITY Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=;
PATHWAYtRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Vertebrata>
SUBUNITForms a complex with @gn(WDR4).
else case <OC:Fungi>
SUBUNITForms a complex with @gn(TRM82).
end case
case <FTTag:phospho>
PTMPhosphorylation at #{Ser-27} inactivates its catalytic activity but does not affect the interaction with @gn(WDR4).
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]

Gene Ontology [?]

GO:0000049; Molecular function:tRNA binding
GO:0008176; Molecular function:tRNA (guanine(46)-N7)-methyltransferase activity
GO:0005634; Cellular component:nucleus
GO:0030488; Biological process:tRNA methylation

Cross-references [?]

Pfam PF02390; Methyltransf_4; 1;
NCBIfam TIGR00091; CHP91; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 107 108 /ligand="S-adenosyl-L-methionine"
BINDING 140 141 /ligand="S-adenosyl-L-methionine"
BINDING 238 240 /ligand="S-adenosyl-L-methionine"
ACT_SITE 163 163 D
BINDING 84 84 /ligand="S-adenosyl-L-methionine"
BINDING 160 160 /ligand="S-adenosyl-L-methionine"
case <OC:Mammalia>
MOD_RES 27 27 /note="Phosphoserine; by PKB and RPS6KA3" phospho S
end case

Additional information [?]

Size range 214-377 amino acids
Related rules None
Fusion Nter: None Cter: None

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