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Annotation rule MF_03055 |
General rule information
[?]
Accession |
MF_03055 |
Dates |
13-MAR-2009 (Created) 20-NOV-2019 (Last updated, Version 7) |
Name |
tRNA_methyltr_TrmB_euk |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=tRNA (guanine-N(7)-)-methyltransferase;
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EC 2.1.1.33;
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AltName:
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Full=tRNA (guanine(46)-N(7))-methyltransferase;
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AltName:
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Full=tRNA(m7G46)-methyltransferase;
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case <OC:Vertebrata>
Protein name |
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Full=Methyltransferase-like protein 1;
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else case <OC:Fungi>
Protein name |
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Full=Transfer RNA methyltransferase 8;
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end case
case <OC:Vertebrata> or <OC:Dictyostelium>
else case <OC:Caenorhabditis>
else case <OC:Fungi>
end case
case <OC:Saccharomyces>
Function |
Methyltransferase that catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity. |
else
Function |
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. |
end case
Catalytic activity |
RHEA:42708: guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine
EC 2.1.1.33
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Pathway |
tRNA modification; N(7)-methylguanine-tRNA biosynthesis. |
case <OC:Vertebrata>
Subunit |
Forms a complex with @gn(WDR4). |
else case <OC:Fungi>
Subunit |
Forms a complex with @gn(TRM82). |
end case
Subcellular location |
Nucleus. |
case <FTTag:phospho>
Ptm |
Phosphorylation at #{Ser-27} inactivates its catalytic activity but does not affect the interaction with @gn(WDR4). |
end case
Similarity |
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. |
case <FTTag:phospho>
end case
GO:0000049; Molecular function: tRNA binding.
GO:0008176; Molecular function: tRNA (guanine-N7-)-methyltransferase activity.
GO:0005634; Cellular component: nucleus.
GO:0030488; Biological process: tRNA methylation.
From: TRMB_HUMAN (Q9UBP6) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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107
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108
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S-adenosyl-L-methionine binding
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E-[IL]
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REGION
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140
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141
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S-adenosyl-L-methionine binding
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N-x
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REGION
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238
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240
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S-adenosyl-L-methionine binding
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[ST]-[ED]-[ED]
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ACT_SITE
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163
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163
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D
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BINDING
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84
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84
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S-adenosyl-L-methionine; via carbonyl oxygen
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G
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BINDING
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160
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160
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S-adenosyl-L-methionine; via carbonyl oxygen
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[LC]
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case <OC:Mammalia>
MOD_RES
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27
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27
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Phosphoserine; by PKB and RPS6KA3
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phospho
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S
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end case
Additional information
[?]
Size range |
214-377 amino acids |
Related rules |
None |
Fusion |
None |