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HAMAP rule MF_03071

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03071
Accession MF_03071
Dates 03-AUG-2016 (Created)
18-MAR-2025 (Last updated, Version 15)
Name CHD8
Scope(s) Eukaryota
Vertebrata
Template(s) Q9HCK8; Q09XV5; Q9JIX5; [ Recover all ]
Triggered by HAMAP; MF_03071 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CHD8
Protein name RecName: Full=Chromodomain-helicase-DNA-binding protein 8;
                 Short=CHD-8;
                 EC=3.6.4.-;
AltName: Full=ATP-dependent helicase CHD8;
Gene name Name=CHD8;

Comments [?]

case <OC:Mammalia>
FUNCTIONATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
SUBUNITInteracts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BACC1, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Interacts with FAM124B.
SUBCELLULAR LOCATIONNucleus. Note=Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
PTMSumoylated.
else
FUNCTIONATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/@gn(TP53)- mediated apoptosis by recruiting histone H1 and preventing p53/@gn(TP53) transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (@gn(CTNNB1)) activity. Negatively regulates @gn(CTNNB1)-targeted gene expression by being recruited specifically to the promoter regions of several @gn(CTNNB1) responsive genes. May also act as a transcription activator by participating in efficient U6 RNA polymerase III transcription.
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
SUBUNITComponent of some MLL1/MLL complex.
SUBCELLULAR LOCATIONNucleus.
end case
SIMILARITYBelongs to the SNF2/RAD54 helicase family. CHD8 subfamily.

Keywords [?]

Activator
ATP-binding
Chromatin regulator
DNA-binding
Helicase
Hydrolase
Nucleotide-binding
Nucleus
Repeat
Repressor
Transcription
Transcription regulation
Wnt signaling pathway
case <FTTag:sumo>
Isopeptide bond
Ubl conjugation
end case

Gene Ontology [?]

GO:0005634; Cellular component:nucleus
GO:0071339; Cellular component:MLL1 complex
GO:0005524; Molecular function:ATP binding
GO:0003677; Molecular function:DNA binding
GO:0003678; Molecular function:DNA helicase activity
GO:0008094; Molecular function:ATP-dependent activity, acting on DNA
GO:0002039; Molecular function:p53 binding
GO:0042393; Molecular function:histone binding
GO:0008013; Molecular function:beta-catenin binding
GO:0006338; Biological process:chromatin remodeling
GO:0016887; Molecular function:ATP hydrolysis activity
GO:0045893; Biological process:positive regulation of DNA-templated transcription
GO:0045892; Biological process:negative regulation of DNA-templated transcription
GO:0030178; Biological process:negative regulation of Wnt signaling pathway
GO:0043066; Biological process:negative regulation of apoptotic process

Cross-references [?]

PROSITE PS00598; CHROMO_1; 2;
PROSITE PS50013; CHROMO_2; 2;
PROSITE PS00690; DEAH_ATP_HELICASE; 1;
PROSITE PS51192; HELICASE_ATP_BIND_1; 1;
PROSITE PS51194; HELICASE_CTER; 1;
PROSITE PS50312; ASP_RICH; 0-unlimited;
PROSITE PS50322; GLN_RICH; 0-unlimited;
PROSITE PS50324; SER_RICH; 0-unlimited;
PROSITE PS50316; HIS_RICH; 0-unlimited;
Pfam PF07533; BRK; 1;
Pfam PF00385; Chromo; 2;
Pfam PF00271; Helicase_C; 1;
Pfam PF00176; SNF2_N; 1;

Features [?]

From: CHD8_HUMAN (Q9HCK8)
Key From To Description Tag Condition FTGroup
case <OC:Mammalia>
REGION 1789 2302 /note="Interaction with FAM124B"
end case
From: CHD8_RAT (Q9JIX5)
Key From To Description Tag Condition FTGroup
case <OC:Mammalia>
CROSSLNK 609 609 /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" sumo K
end case

Additional information [?]

Size range 2184-2594 amino acids
Related rules None
Fusion Nter: None Cter: None



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