HAMAP rule MF_03101
General rule information
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| Accession | MF_03101 |
| Dates | 14-AUG-2009 (Created)
19-NOV-2022 (Last updated, Version 13) |
| Name | Deoxyhypusine_hydroxylase |
| Scope(s) |
Eukaryota |
| Template(s) | Q9BU89 (DOHH_HUMAN); P47120 (DOHH_YEAST); Q9V9U4 (DOHH_DROME); [ Recover all ] |
| Triggered by |
HAMAP; MF_03101 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DOHH |
| case <OC:Saccharomyces> | |
| Protein name | RecName: Full=Deoxyhypusine hydroxylase; Short=DOHH; EC=1.14.99.29; AltName: Full=Deoxyhypusine dioxygenase; AltName: Full=Deoxyhypusine monooxygenase; AltName: Full=Ligand of eIF5A protein 1; |
| else | |
| Protein name | RecName: Full=Deoxyhypusine hydroxylase; Short=DOHH; EC=1.14.99.29; AltName: Full=Deoxyhypusine dioxygenase; AltName: Full=Deoxyhypusine monooxygenase; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=DOHH; |
| else case <OC:Caenorhabditis> | |
| Gene name | Name=dohh-1; Synonyms=tag-242; |
| else case <OC:Drosophilidae> | |
| Gene name | Name=nero; |
| else case <OC:Fungi> | |
| Gene name | Name=LIA1; |
| end case | |
Comments
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| case <OC:Drosophilidae> | |
| FUNCTION | Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L- lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A- mediated translational control. |
| else | |
| FUNCTION | Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L- lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]- hypusine + A + H2O; Xref=Rhea:RHEA:14101, Rhea:RHEA-COMP:10144, Rhea:RHEA-COMP:12592, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499, ChEBI:CHEBI:82657, ChEBI:CHEBI:91175; EC=1.14.99.29; |
| COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 2 Fe(2+) ions per subunit.; |
| PATHWAY | Protein modification; eIF5A hypusination. |
| case <OC:Fungi> | |
| SUBCELLULAR LOCATION | Cytoplasm. Nucleus. |
| else case <OC:Drosophilidae> | |
| SUBCELLULAR LOCATION | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In larval imaginal disk and Garland cells. |
| end case | |
| SIMILARITY | Belongs to the deoxyhypusine hydroxylase family. |
Keywords
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| Hypusine biosynthesis | |
| Iron | |
| Metal-binding | |
| Monooxygenase | |
| Oxidoreductase | |
| case <OC:Fungi> | |
| Cytoplasm | |
| Nucleus | |
| end case | |
| case <OC:Drosophilidae> | |
| Endoplasmic reticulum | |
| Membrane | |
| end case | |
| case <FTTag:acetyl> | |
| Acetylation | |
| end case | |
Gene Ontology
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| GO:0019135; Molecular function:deoxyhypusine monooxygenase activity | |
| GO:0008612; Biological process:peptidyl-lysine modification to peptidyl-hypusine | |
| case <OCellular component:Fungi> | |
| GO:0005737; Cellular component:cytoplasm | |
| GO:0005634; Cellular component:nucleus | |
| end case | |
| case <OCellular component:Drosophilidae> | |
| GO:0042406; Cellular component:extrinsic component of endoplasmic reticulum membrane | |
| end case | |
Cross-references
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Features
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| From: DOHH_HUMAN (Q9BU89) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 56 | 56 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
H | ||||||||
| BINDING | 57 | 57 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
E | ||||||||
| BINDING | 89 | 89 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
H | ||||||||
| BINDING | 90 | 90 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="1" |
E | ||||||||
| BINDING | 207 | 207 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
H | ||||||||
| BINDING | 208 | 208 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
E | ||||||||
| BINDING | 240 | 240 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
H | ||||||||
| BINDING | 241 | 241 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" /ligand_label="2" |
E | ||||||||
| From: DOHH_YEAST (P47120) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Saccharomyces> | ||||||||||||
| INIT_MET | 1 | 1 | /note="Removed" | M | ||||||||
| MOD_RES | 2 | 2 | /note="N-acetylserine" | acetyl | S | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 282-411 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |