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HAMAP rule MF_03101

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General rule information [?]

Accession MF_03101
Dates 14-AUG-2009 (Created)
19-NOV-2022 (Last updated, Version 13)
Name Deoxyhypusine_hydroxylase
Scope(s) Eukaryota
Template(s) Q9BU89 (DOHH_HUMAN); P47120 (DOHH_YEAST); Q9V9U4 (DOHH_DROME); [ Recover all ]
Triggered by HAMAP; MF_03101 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DOHH
case <OC:Saccharomyces>
Protein name RecName: Full=Deoxyhypusine hydroxylase;
                 Short=DOHH;
                 EC=1.14.99.29;
AltName: Full=Deoxyhypusine dioxygenase;
AltName: Full=Deoxyhypusine monooxygenase;
AltName: Full=Ligand of eIF5A protein 1;
else
Protein name RecName: Full=Deoxyhypusine hydroxylase;
                 Short=DOHH;
                 EC=1.14.99.29;
AltName: Full=Deoxyhypusine dioxygenase;
AltName: Full=Deoxyhypusine monooxygenase;
end case
case <OC:Vertebrata>
Gene name Name=DOHH;
else case <OC:Caenorhabditis>
Gene name Name=dohh-1; Synonyms=tag-242;
else case <OC:Drosophilidae>
Gene name Name=nero;
else case <OC:Fungi>
Gene name Name=LIA1;
end case

Comments [?]

case <OC:Drosophilidae>
FUNCTIONCatalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L- lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A- mediated translational control.
else
FUNCTIONCatalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L- lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
end case
CATALYTIC ACTIVITY Reaction=[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]- hypusine + A + H2O; Xref=Rhea:RHEA:14101, Rhea:RHEA-COMP:10144, Rhea:RHEA-COMP:12592, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499, ChEBI:CHEBI:82657, ChEBI:CHEBI:91175; EC=1.14.99.29;
COFACTOR Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 2 Fe(2+) ions per subunit.;
PATHWAYProtein modification; eIF5A hypusination.
case <OC:Fungi>
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
else case <OC:Drosophilidae>
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In larval imaginal disk and Garland cells.
end case
SIMILARITYBelongs to the deoxyhypusine hydroxylase family.

Keywords [?]

Hypusine biosynthesis
Iron
Metal-binding
Monooxygenase
Oxidoreductase
case <OC:Fungi>
Cytoplasm
Nucleus
end case
case <OC:Drosophilidae>
Endoplasmic reticulum
Membrane
end case
case <FTTag:acetyl>
Acetylation
end case

Gene Ontology [?]

GO:0019135; Molecular function:deoxyhypusine monooxygenase activity
GO:0008612; Biological process:peptidyl-lysine modification to peptidyl-hypusine
case <OCellular component:Fungi>
GO:0005737; Cellular component:cytoplasm
GO:0005634; Cellular component:nucleus
end case
case <OCellular component:Drosophilidae>
GO:0042406; Cellular component:extrinsic component of endoplasmic reticulum membrane
end case

Cross-references [?]

PROSITE PS50077; HEAT_REPEAT; 1-2;
Pfam PF03130; HEAT_PBS; 3-5;
Pfam PF02985; HEAT; 1;

Features [?]

From: DOHH_HUMAN (Q9BU89)
Key From To Description Tag Condition FTGroup
BINDING 56 56 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="1"
H
BINDING 57 57 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="1"
E
BINDING 89 89 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="1"
H
BINDING 90 90 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="1"
E
BINDING 207 207 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="2"
H
BINDING 208 208 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="2"
E
BINDING 240 240 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="2"
H
BINDING 241 241 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
/ligand_label="2"
E
From: DOHH_YEAST (P47120)
Key From To Description Tag Condition FTGroup
case <OC:Saccharomyces>
INIT_MET 1 1 /note="Removed" M
MOD_RES 2 2 /note="N-acetylserine" acetyl S
end case

Additional information [?]

Size range 282-411 amino acids
Related rules None
Fusion Nter: None Cter: None



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