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Annotation rule MF_03101
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General rule information [?]

Accession MF_03101
Dates 14-AUG-2009 (Created)
20-NOV-2019 (Last updated, Version 11)
Name Deoxyhypusine_hydroxylase
Scope
Eukaryota
Templates Q9BU89 (DOHH_HUMAN); P47120 (DOHH_YEAST); Q9V9U4 (DOHH_DROME): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DOHH
case <OC:Saccharomyces>
Protein name
RecName: Full=Deoxyhypusine hydroxylase;
Short=DOHH;
EC 1.14.99.29;
AltName: Full=Deoxyhypusine dioxygenase;
AltName: Full=Deoxyhypusine monooxygenase;
AltName: Full=Ligand of eIF5A protein 1;
else
Protein name
RecName: Full=Deoxyhypusine hydroxylase;
Short=DOHH;
EC 1.14.99.29;
AltName: Full=Deoxyhypusine dioxygenase;
AltName: Full=Deoxyhypusine monooxygenase;
end case
case <OC:Vertebrata>
Gene name
DOHH
else case <OC:Caenorhabditis>
Gene name
dohh-1, tag-242
else case <OC:Drosophilidae>
Gene name
nero
else case <OC:Fungi>
Gene name
LIA1
end case

Comments [?]

case <OC:Drosophilidae>
Function Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A-mediated translational control.
else
Function Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
end case
Catalytic activity RHEA:14101: [eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O
EC 1.14.99.29
Cofactor Fe(2+)
Note: Binds 2 Fe(2+) ions per subunit.
Pathway Protein modification; eIF5A hypusination.
case <OC:Fungi>
Subcellular location Cytoplasm. Nucleus.
else case <OC:Drosophilidae>
Subcellular location Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In larval imaginal disk and Garland cells.
end case
Similarity Belongs to the deoxyhypusine hydroxylase family.

Keywords [?]

case <OC:Fungi>
end case
case <OC:Drosophilidae>
end case
case <FTTag:acetyl>
end case

Gene Ontology [?]

GO:0019135; Molecular function: deoxyhypusine monooxygenase activity.
GO:0008612; Biological process: peptidyl-lysine modification to peptidyl-hypusine.
case <OC:Fungi>
GO:0005737; Cellular component: cytoplasm.
GO:0005634; Cellular component: nucleus.
end case
case <OC:Drosophilidae>
GO:0042406; Cellular component: extrinsic component of endoplasmic reticulum membrane.
end case

Cross-references [?]

PROSITE PS50077; HEAT_REPEAT; 1-2;
Pfam PF03130; HEAT_PBS; 3-5; trigger=Yes;
PF02985; HEAT; 1;

Features [?]

From: DOHH_HUMAN (Q9BU89)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     56     56       Iron 1     H  
METAL     57     57       Iron 1     E  
METAL     89     89       Iron 1     H  
METAL     90     90       Iron 1     E  
METAL     207     207       Iron 2     H  
METAL     208     208       Iron 2     E  
METAL     240     240       Iron 2     H  
METAL     241     241       Iron 2     E  
case <OC:Saccharomyces>
From: DOHH_YEAST (P47120)
INIT_MET     1     1       Removed     M  
MOD_RES     2     2       N-acetylserine   acetyl   S  
end case

Additional information [?]

Size range 282-411 amino acids
Related rules None
Fusion None