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HAMAP rule MF_03110

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General rule information [?]

Accession MF_03110
Dates 8-OCT-2009 (Created)
20-NOV-2019 (Last updated, Version 4)
Name Endonuc_su_Slx4
Scope
Eukaryota; Fungi
Templates Q12098 (SLX4_YEAST); Q9P6M0 (SLX4_SCHPO): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SLX4
Protein name
RecName: Full=Structure-specific endonuclease subunit @gn(SLX4);
Gene name
SLX4

Comments [?]

case <OC:Saccharomyces>
Function Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107.
Subunit Forms a heterodimer with SLX1. Interacts with RAD1; catalytic subunit of the RAD1-RAD10 endonuclease. Interacts with RTT107.
Subcellular location Nucleus. Cytoplasm.
Ptm Phosphorylated by ATR (MEC1) and ATM (TEL1) upon DNA damage. This appears to be required for the function with the RAD1-RAD10 endonuclease.
else case <OC:Fungi> and not <OC:Saccharomyces>
Function Regulatory subunit of the @gn(SLX1)-@gn(SLX4) structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.
Subunit Forms a heterodimer with @gn(SLX1).
Subcellular location Nucleus.
Ptm Phosphorylated in response to DNA damage.
end case
Similarity Belongs to the SLX4 family.

Keywords [?]

DNA damage
DNA recombination
DNA repair
Nucleus
Phosphoprotein
case <OC:Saccharomyces>
Cytoplasm
end case

Gene Ontology [?]

GO:0005634; Cellular component: nucleus.
GO:0033557; Cellular component: Slx1-Slx4 complex.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF09494; Slx4; 1;

Additional information [?]

Size range 419-1311 amino acids
Related rules None
Fusion None


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