HAMAP rule MF_03110
General rule information
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| Accession | MF_03110 |
| Dates | 8-OCT-2009 (Created)
20-NOV-2019 (Last updated, Version 4) |
| Name | Endonuc_su_Slx4 |
| Scope(s) |
Eukaryota Fungi |
| Template(s) | Q12098 (SLX4_YEAST); Q9P6M0 (SLX4_SCHPO); [ Recover all ] |
| Triggered by |
HAMAP; MF_03110 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SLX4 |
| Protein name | RecName: Full=Structure-specific endonuclease subunit @gn(SLX4); |
| Gene name | Name=SLX4; |
Comments
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| case <OC:Saccharomyces> | |
| FUNCTION | Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re- starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error- free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107. |
| SUBUNIT | Forms a heterodimer with SLX1. Interacts with RAD1; catalytic subunit of the RAD1-RAD10 endonuclease. Interacts with RTT107. |
| SUBCELLULAR LOCATION | Nucleus. Cytoplasm. |
| PTM | Phosphorylated by ATR (MEC1) and ATM (TEL1) upon DNA damage. This appears to be required for the function with the RAD1-RAD10 endonuclease. |
| else case <OC:Fungi> and not <OC:Saccharomyces> | |
| FUNCTION | Regulatory subunit of the @gn(SLX1)-@gn(SLX4) structure- specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
| SUBUNIT | Forms a heterodimer with @gn(SLX1). |
| SUBCELLULAR LOCATION | Nucleus. |
| PTM | Phosphorylated in response to DNA damage. |
| end case | |
| SIMILARITY | Belongs to the SLX4 family. |
Keywords
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| DNA damage | |
| DNA recombination | |
| DNA repair | |
| Nucleus | |
| Phosphoprotein | |
| case <OC:Saccharomyces> | |
| Cytoplasm | |
| end case | |
Gene Ontology
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| GO:0005634; Cellular component:nucleus |
| GO:0033557; Cellular component:Slx1-Slx4 complex |
| GO:0006310; Biological process:DNA recombination |
| GO:0006281; Biological process:DNA repair |
Cross-references
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| Pfam | PF09494; Slx4; 1; |
Features
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Additional information
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| Size range | 419-1311 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |