HAMAP rule MF_03110
General rule information
[?]
Accession | MF_03110 |
Dates | 8-OCT-2009 (Created) 20-NOV-2019 (Last updated, Version 4) |
Name | Endonuc_su_Slx4 |
Scope | Eukaryota; Fungi |
Templates | Q12098 (SLX4_YEAST); Q9P6M0 (SLX4_SCHPO): [Recover all] |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Gene name |
|
Comments
[?]
case <OC:Saccharomyces>
Function | Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107. |
Subunit | Forms a heterodimer with SLX1. Interacts with RAD1; catalytic subunit of the RAD1-RAD10 endonuclease. Interacts with RTT107. |
Subcellular location | Nucleus. Cytoplasm. |
Ptm | Phosphorylated by ATR (MEC1) and ATM (TEL1) upon DNA damage. This appears to be required for the function with the RAD1-RAD10 endonuclease. |
else case <OC:Fungi> and not <OC:Saccharomyces>
Function | Regulatory subunit of the @gn(SLX1)-@gn(SLX4) structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
Subunit | Forms a heterodimer with @gn(SLX1). |
Subcellular location | Nucleus. |
Ptm | Phosphorylated in response to DNA damage. |
end case
Similarity | Belongs to the SLX4 family. |
Keywords
[?]
case <OC:Saccharomyces>
end case
Gene Ontology
[?]
GO:0005634; Cellular component: nucleus.
GO:0033557; Cellular component: Slx1-Slx4 complex.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0033557; Cellular component: Slx1-Slx4 complex.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
Cross-references
[?]
Pfam | PF09494; Slx4; 1; |
Additional information
[?]
Size range | 419-1311 amino acids |
Related rules | None |
Fusion | None |