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Annotation rule MF_03110 |
General rule information
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Accession |
MF_03110 |
Dates |
8-OCT-2009 (Created) 20-NOV-2019 (Last updated, Version 4) |
Propagated annotation
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Identifier, protein and gene names
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Protein name |
RecName:
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Full=Structure-specific endonuclease subunit @gn(SLX4);
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case <OC:Saccharomyces>
Function |
Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107. |
Subunit |
Forms a heterodimer with SLX1. Interacts with RAD1; catalytic subunit of the RAD1-RAD10 endonuclease. Interacts with RTT107. |
Subcellular location |
Nucleus. Cytoplasm. |
Ptm |
Phosphorylated by ATR (MEC1) and ATM (TEL1) upon DNA damage. This appears to be required for the function with the RAD1-RAD10 endonuclease. |
else case <OC:Fungi> and not <OC:Saccharomyces>
Function |
Regulatory subunit of the @gn(SLX1)-@gn(SLX4) structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
Subunit |
Forms a heterodimer with @gn(SLX1). |
Subcellular location |
Nucleus. |
Ptm |
Phosphorylated in response to DNA damage. |
end case
Similarity |
Belongs to the SLX4 family. |
case <OC:Saccharomyces>
end case
Additional information
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Size range |
419-1311 amino acids |
Related rules |
None |
Fusion |
None |