HAMAP rule MF_03118
General rule information
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| Accession | MF_03118 |
| Dates | 1-APR-2010 (Created)
24-MAY-2024 (Last updated, Version 16) |
| Name | Salvage_MtnBC |
| Scope(s) |
Eukaryota Viridiplantae |
| Template(s) | Q9FN41 (MTBC_ARATH); [ Recover all ] |
| Triggered by |
HAMAP; MF_03118 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MTBC |
| Protein name | RecName: Full=Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1; Includes: RecName: Full=Methylthioribulose-1-phosphate dehydratase; Short=MTRu-1-P dehydratase; EC=4.2.1.109; Includes: RecName: Full=Enolase-phosphatase E1; EC=3.1.3.77; AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase; |
Comments
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| CATALYTIC ACTIVITY | Reaction=S-methyl-5-thio-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3- dioxopentyl phosphate + H2O; Xref=Rhea:RHEA:15549, ChEBI:CHEBI:15377, ChEBI:CHEBI:58548, ChEBI:CHEBI:58828; EC=4.2.1.109; |
| CATALYTIC ACTIVITY | Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate; Xref=Rhea:RHEA:21700, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:49252, ChEBI:CHEBI:58828; EC=3.1.3.77; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6. |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6. |
| SIMILARITY | In the N-terminal section; belongs to the aldolase class II family. MtnB subfamily. |
| SIMILARITY | In the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family. |
Keywords
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| Amino-acid biosynthesis | |
| Hydrolase | |
| Lyase | |
| case <FTGroup:1> | |
| Magnesium | |
| end case | |
| Metal-binding | |
| Methionine biosynthesis | |
| Multifunctional enzyme | |
| Zinc | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0008270; Molecular function:zinc ion binding | |
| GO:0043715; Molecular function:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity | |
| GO:0043716; Molecular function:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity | |
| GO:0046570; Molecular function:methylthioribulose 1-phosphate dehydratase activity | |
| GO:0019509; Biological process:L-methionine salvage from methylthioadenosine | |
Cross-references
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| Pfam | PF00596; Aldolase_II; 1; |
| Pfam | PF00702; Hydrolase; 1; |
| NCBIfam | TIGR01691; enolase-ppase; 1; |
| NCBIfam | TIGR01549; HAD-SF-IA-v1; 1; |
| NCBIfam | TIGR03328; salvage_mtnB; 1; |
Features
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| From: MTBC_ARATH (Q9FN41) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 237 | /note="Methylthioribulose-1-phosphate dehydratase" | |||||||||
| REGION | 268 | Cter | /note="Enolase-phosphatase E1" | |||||||||
| BINDING | 406 | 407 | /ligand="substrate" /ligand_label="2" /ligand_note="for enolase-phosphatase activity" |
S-S | ||||||||
| ACT_SITE | 152 | 152 | /note="Proton donor/acceptor; for methylthioribulose-1- phosphate dehydratase activity" | E | ||||||||
| BINDING | 127 | 127 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 129 | 129 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 202 | 202 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 109 | 109 | /ligand="substrate" /ligand_label="1" /ligand_note="for methylthioribulose-1-phosphate dehydratase activity" |
C | ||||||||
| BINDING | 271 | 271 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 273 | 273 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 466 | 466 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 440 | 440 | /ligand="substrate" /ligand_label="2" /ligand_note="for enolase-phosphatase activity" |
K | ||||||||
Additional information
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| Size range | 504-531 amino acids |
| Related rules |
MF_03116 MF_03117 |
| Fusion | Nter: None Cter: None |