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Annotation rule MF_03118
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General rule information [?]

Accession MF_03118
Dates 1-APR-2010 (Created)
6-MAY-2020 (Last updated, Version 10)
Name Salvage_MtnBC
Scope
Eukaryota; Viridiplantae
Template Q9FN41 (MTBC_ARATH)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MTBC
Protein name
RecName: Full=Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1;
Includes:
RecName: Full=Methylthioribulose-1-phosphate dehydratase;
Short=MTRu-1-P dehydratase;
EC 4.2.1.109;
Includes:
RecName: Full=Enolase-phosphatase E1;
EC 3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;

Comments [?]

Catalytic activity RHEA:15549: S-methyl-5-thio-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O
EC 4.2.1.109
RHEA:21700: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate
EC 3.1.3.77
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Similarity In the N-terminal section; belongs to the aldolase class II family. MtnB subfamily.
In the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0008270; Molecular function: zinc ion binding.
GO:0043715; Molecular function: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity.
GO:0043716; Molecular function: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity.
GO:0046570; Molecular function: methylthioribulose 1-phosphate dehydratase activity.
GO:0019284; Biological process: L-methionine salvage from S-adenosylmethionine.

Cross-references [?]

Pfam PF00596; Aldolase_II; 1;
PF00702; Hydrolase; 1;
TIGRFAMs TIGR01691; enolase-ppase; 1;
TIGR01549; HAD-SF-IA-v1; 1;
TIGR03328; salvage_mtnB; 1;

Features [?]

From: MTBC_ARATH (Q9FN41)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     237       Methylthioribulose-1-phosphate dehydratase        
REGION     268     Cter       Enolase-phosphatase E1        
REGION     406     407       Substrate binding; for enolase-phosphatase activity     S-S  
ACT_SITE     152     152       Proton donor/acceptor; for methylthioribulose-1-phosphate dehydratase activity     E  
METAL     127     127       Zinc; via tele nitrogen     H  
METAL     129     129       Zinc; via tele nitrogen     H  
METAL     202     202       Zinc; via tele nitrogen     H  
BINDING     109     109       Substrate; for methylthioribulose-1-phosphate dehydratase activity     C  
METAL     271     271       Magnesium     D   1
METAL     273     273       Magnesium; via carbonyl oxygen     E   1
METAL     466     466       Magnesium     D   1
BINDING     440     440       Substrate; for enolase-phosphatase activity     K  

Additional information [?]

Size range 504-531 amino acids
Related rules MF_03116 (MTNB); MF_03117 (ENOPH)
Fusion None