HAMAP logo

HAMAP rule MF_03118

Send feedback

General rule information [?]

Accession MF_03118
Dates 1-APR-2010 (Created)
24-MAY-2024 (Last updated, Version 16)
Name Salvage_MtnBC
Scope(s) Eukaryota
Viridiplantae
Template(s) Q9FN41 (MTBC_ARATH); [ Recover all ]
Triggered by HAMAP; MF_03118 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MTBC
Protein name RecName: Full=Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1;
                 Includes:
RecName: Full=Methylthioribulose-1-phosphate dehydratase;
                 Short=MTRu-1-P dehydratase;
                 EC=4.2.1.109;
                 Includes:
RecName: Full=Enolase-phosphatase E1;
                 EC=3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;

Comments [?]

CATALYTIC ACTIVITY Reaction=S-methyl-5-thio-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3- dioxopentyl phosphate + H2O; Xref=Rhea:RHEA:15549, ChEBI:CHEBI:15377, ChEBI:CHEBI:58548, ChEBI:CHEBI:58828; EC=4.2.1.109;
CATALYTIC ACTIVITY Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate; Xref=Rhea:RHEA:21700, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:49252, ChEBI:CHEBI:58828; EC=3.1.3.77;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
SIMILARITYIn the N-terminal section; belongs to the aldolase class II family. MtnB subfamily.
SIMILARITYIn the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

Keywords [?]


Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0008270; Molecular function:zinc ion binding
GO:0043715; Molecular function:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity
GO:0043716; Molecular function:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity
GO:0046570; Molecular function:methylthioribulose 1-phosphate dehydratase activity
GO:0019509; Biological process:L-methionine salvage from methylthioadenosine

Cross-references [?]

Pfam PF00596; Aldolase_II; 1;
Pfam PF00702; Hydrolase; 1;
NCBIfam TIGR01691; enolase-ppase; 1;
NCBIfam TIGR01549; HAD-SF-IA-v1; 1;
NCBIfam TIGR03328; salvage_mtnB; 1;

Features [?]

From: MTBC_ARATH (Q9FN41)
Key From To Description Tag Condition FTGroup
REGION Nter 237 /note="Methylthioribulose-1-phosphate dehydratase"
REGION 268 Cter /note="Enolase-phosphatase E1"
BINDING 406 407 /ligand="substrate"
/ligand_label="2"
/ligand_note="for enolase-phosphatase activity"
S-S
ACT_SITE 152 152 /note="Proton donor/acceptor; for methylthioribulose-1- phosphate dehydratase activity" E
BINDING 127 127 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
H
BINDING 129 129 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
H
BINDING 202 202 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
H
BINDING 109 109 /ligand="substrate"
/ligand_label="1"
/ligand_note="for methylthioribulose-1-phosphate dehydratase activity"
C
BINDING 271 271 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 273 273 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
E 1
BINDING 466 466 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 440 440 /ligand="substrate"
/ligand_label="2"
/ligand_note="for enolase-phosphatase activity"
K

Additional information [?]

Size range 504-531 amino acids
Related rules MF_03116
MF_03117
Fusion Nter: None Cter: None



View rule in raw text format (no links)