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HAMAP rule MF_03124

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03124
Accession MF_03124
Dates 22-AUG-2012 (Created)
12-JUN-2025 (Last updated, Version 15)
Name ArgJ_euk
Scope(s) Eukaryota
Template(s) Q04728; Q3C251; [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01106 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ARGJ
case <OC:Viridiplantae>
Protein name RecName: Full=Arginine biosynthesis bifunctional protein ArgJ, chloroplastic;
                 Includes:
RecName: Full=Glutamate N-acetyltransferase;
                 Short=GAT;
                 EC=2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
                 Short=OATase;
AltName: Full=Ornithine transacetylase;
                 Includes:
RecName: Full=Amino-acid acetyltransferase;
                 EC=2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
                 Short=AGS;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
                 Flags: Precursor;
else
Protein name RecName: Full=Arginine biosynthesis bifunctional protein ArgJ, mitochondrial;
                 Includes:
RecName: Full=Glutamate N-acetyltransferase;
                 Short=GAT;
                 EC=2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
                 Short=OATase;
AltName: Full=Ornithine transacetylase;
                 Includes:
RecName: Full=Amino-acid acetyltransferase;
                 EC=2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
                 Short=AGS;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
                 Flags: Precursor;
end case
case <OC:Saccharomyces>
Gene name Name=ARG7;
end case

Comments [?]

FUNCTIONCatalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.
CATALYTIC ACTIVITY Reaction=N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine; Xref=Rhea:RHEA:15349, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:46911, ChEBI:CHEBI:57805; EC=2.3.1.35;
CATALYTIC ACTIVITY Reaction=L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+); Xref=Rhea:RHEA:24292, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.1;
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl- L-ornithine from L-glutamate: step 1/4.
SUBUNITHeterodimer of an alpha and a beta chain.
case <OC:Viridiplantae>
SUBCELLULAR LOCATIONPlastid, chloroplast.
else
SUBCELLULAR LOCATIONMitochondrion matrix.
end case
case not <OC:Viridiplantae>
PTMThe alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.
end case
case not <OC:Saccharomyces> and not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the ArgJ family.

Keywords [?]

Acyltransferase
Amino-acid biosynthesis
Arginine biosynthesis
Autocatalytic cleavage
case <OC:Viridiplantae>
Chloroplast
Plastid
else
Mitochondrion
end case
Multifunctional enzyme
Transferase
case <OC:Saccharomyces>
Transit peptide
end case

Gene Ontology [?]

GO:0006526; Biological process:L-arginine biosynthetic process
GO:0004358; Molecular function:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor
GO:0004042; Molecular function:L-glutamate N-acetyltransferase activity
case <OCellular component:Viridiplantae>
GO:0009507; Cellular component:chloroplast
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005759; Cellular component:mitochondrial matrix
end case

Cross-references [?]

Pfam PF01960; ArgJ; 1;
NCBIfam TIGR00120; ArgJ; 1;
General TransitM; -; 0-1;

Features [?]

From: ARGJ_YEAST (Q04728)
Key From To Description Tag Condition FTGroup
case <OC:Saccharomyces>
TRANSIT Nter 8 /note="Mitochondrion"
CHAIN 9 214 /note="Arginine biosynthesis bifunctional protein ArgJ alpha chain"
else
CHAIN Nter 214 /note="Arginine biosynthesis bifunctional protein ArgJ alpha chain"
end case
CHAIN 215 Cter /note="Arginine biosynthesis bifunctional protein ArgJ beta chain"
SITE 214 215 /note="Cleavage; by autolysis" A-T
From: ARGJ_MYCTU (P9WPZ3)
Key From To Description Tag Condition FTGroup
ACT_SITE 200 200 /note="Nucleophile" [TS]
BINDING 166 166 /ligand="substrate" T
BINDING 189 189 /ligand="substrate" K
BINDING 200 200 /ligand="substrate" [TS]
BINDING 280 280 /ligand="substrate" E
BINDING 399 399 /ligand="substrate" N
BINDING 404 404 /ligand="substrate" [ST]
SITE 127 127 /note="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" T
SITE 128 128 /note="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" G

Additional information [?]

Size range 428-520 amino acids
Related rules None
Fusion Nter: None Cter: None



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