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HAMAP rule MF_03124

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General rule information [?]

Accession MF_03124
Dates 20-JUL-2010 (Created)
1-JUN-2023 (Last updated, Version 11)
Name ArgJ_euk
Scope
Eukaryota
Templates Q04728 (ARGJ_YEAST); Q3C251 (ARGJ_CITLA): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ARGJ
case <OC:Viridiplantae>
Protein name
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ, chloroplastic;
Includes:
RecName: Full=Glutamate N-acetyltransferase;
Short=GAT;
EC 2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
Short=OATase;
AltName: Full=Ornithine transacetylase;
Includes:
RecName: Full=Amino-acid acetyltransferase;
EC 2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
Short=AGS;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
Flags: Precursor;
else
Protein name
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ, mitochondrial;
Includes:
RecName: Full=Glutamate N-acetyltransferase;
Short=GAT;
EC 2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
Short=OATase;
AltName: Full=Ornithine transacetylase;
Includes:
RecName: Full=Amino-acid acetyltransferase;
EC 2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
Short=AGS;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
Flags: Precursor;
end case
case <OC:Saccharomyces>
Gene name
ARG7
end case

Comments [?]

Function Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.
Catalytic activity RHEA:15349: L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate
EC 2.3.1.35
RHEA:24292: acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate
EC 2.3.1.1
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.
Subunit Heterodimer of an alpha and a beta chain.
case <OC:Viridiplantae>
Subcellular location Plastid, chloroplast.
else
Subcellular location Mitochondrion matrix.
end case
case not <OC:Viridiplantae>
Ptm The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.
end case
case not <OC:Saccharomyces> and not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the ArgJ family.

Keywords [?]

Acyltransferase
Amino-acid biosynthesis
Arginine biosynthesis
Autocatalytic cleavage
case <OC:Viridiplantae>
Chloroplast
Plastid
else
Mitochondrion
end case
Multifunctional enzyme
Transferase
case <OC:Saccharomyces>
Transit peptide
end case

Gene Ontology [?]

GO:0006526; Biological process: arginine biosynthetic process.
GO:0004358; Molecular function: glutamate N-acetyltransferase activity.
GO:0004042; Molecular function: acetyl-CoA:L-glutamate N-acetyltransferase activity.
case <OC:Viridiplantae>
GO:0009507; Cellular component: chloroplast.
else
GO:0005759; Cellular component: mitochondrial matrix.
end case

Cross-references [?]

Pfam PF01960; ArgJ; 1;
NCBIfam TIGR00120; ArgJ; 1;

Computed features [?]

case not <OC:Saccharomyces>
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

case <OC:Saccharomyces>
From: ARGJ_YEAST (Q04728)
Key     From     To       Description   Tag   Condition   FTGroup
TRANSIT     Nter     8       Mitochondrion        
CHAIN     9     214       Arginine biosynthesis bifunctional protein ArgJ alpha chain        
else
CHAIN     Nter     214       Arginine biosynthesis bifunctional protein ArgJ alpha chain        
end case
CHAIN     215     Cter       Arginine biosynthesis bifunctional protein ArgJ beta chain        
SITE     214     215       Cleavage; by autolysis     A-T  
From: ARGJ_MYCTU (P9WPZ3)
ACT_SITE     200     200       Nucleophile     [TS]  
BINDING     166     166       /ligand="substrate     T  
BINDING     189     189       /ligand="substrate     K  
BINDING     200     200       /ligand="substrate     [TS]  
BINDING     280     280       /ligand="substrate     E  
BINDING     399     399       /ligand="substrate     N  
BINDING     404     404       /ligand="substrate     [ST]  
SITE     127     127       Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole     T  
SITE     128     128       Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole     G  

Additional information [?]

Size range 428-520 amino acids
Related rules None
Fusion None


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