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Annotation rule MF_03125
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General rule information [?]

Accession MF_03125
Dates 13-AUG-2010 (Created)
20-NOV-2019 (Last updated, Version 9)
Name Adenylosucc_synth_euk
Scope
Eukaryota; except Vertebrata
Plastid
Templates Q9U8D3 (PURA_PLAFA); P0A7D4 (PURA_ECOLI); Q96529 (PURA_ARATH): [Recover all]
case <OC:Eukaryota> or <OG:Plastid>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PURA
case <OG:Organellar chromatophore>
Protein name
RecName: Full=Adenylosuccinate synthetase, organellar chromatophore;
Short=AMPSase;
Short=AdSS;
EC 6.3.4.4;
AltName: Full=IMP--aspartate ligase;
else case <OC:Viridiplantae>
Protein name
RecName: Full=Adenylosuccinate synthetase, chloroplastic;
Short=AMPSase;
Short=AdSS;
EC 6.3.4.4;
AltName: Full=IMP--aspartate ligase;
else
Protein name
RecName: Full=Adenylosuccinate synthetase;
Short=AMPSase;
Short=AdSS;
EC 6.3.4.4;
AltName: Full=IMP--aspartate ligase;
end case
case <OC:Viridiplantae>
Gene name
PURA
else case <OC:Caenorhabditis>
Gene name
adss-1
else case <OC:Saccharomyces>
Gene name
ADE12
end case

Comments [?]

case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma>
Function Plays an important role in the salvage pathway for purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
else
Function Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
end case
Catalytic activity RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit Homodimer.
case <OG:Organellar chromatophore>
Subcellular location Plastid, organellar chromatophore.
else case <OC:Viridiplantae>
Subcellular location Plastid, chloroplast.
else
Subcellular location Cytoplasm.
end case
case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma>
Miscellaneous Parasitic protozoa lack the de novo purine biosynthesis pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements.
end case
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the adenylosuccinate synthetase family.

Keywords [?]

case <OG:Organellar chromatophore>
else case <OC:Viridiplantae>
else
end case

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0006167; Biological process: AMP biosynthetic process.
case <OG:Organellar chromatophore>
GO:0070111; Cellular component: organellar chromatophore.
else case <OC:Viridiplantae>
GO:0009507; Cellular component: chloroplast.
else
GO:0005737; Cellular component: cytoplasm.
end case

Cross-references [?]

PROSITE PS01266; ADENYLOSUCCIN_SYN_1; 1;
PS00513; ADENYLOSUCCIN_SYN_2; 1;
Pfam PF00709; Adenylsucc_synt; 1;
TIGRFAMs TIGR00184; PurA; 1;

Computed features [?]

case <OC:Viridiplantae>
General TransitC; -; 0-1; trigger=yes;
end case

Features [?]

From: PURA_PLAFA (Q9U8D3)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     25     31       GTP     G-D-E-G-K-G-K  
NP_BIND     53     55       GTP     G-H-[EST]  
NP_BIND     339     341       GTP     K-[ILMV]-D  
NP_BIND     425     427       GTP     G-[INTV]-G  
REGION     26     29       IMP binding     D-E-G-K  
REGION     51     54       IMP binding     N-[AS]-G-H  
REGION     307     313       Substrate binding     x-x-T-x-[KR]-x-R  
ACT_SITE     26     26       Proton acceptor     D  
ACT_SITE     54     54       Proton donor     H  
METAL     26     26       Magnesium     D  
METAL     53     53       Magnesium; via carbonyl oxygen     G  
BINDING     141     141       IMP     T  
BINDING     155     155       IMP; shared with dimeric partner     [KR]  
BINDING     232     232       IMP     [QN]  
BINDING     247     247       IMP     T  
BINDING     311     311       IMP     [KR]  
BINDING     313     313       GTP     R  
case <OC:Plasmodium>
BINDING     62     62       GTP     K  
BINDING     307     307       GTP     T  
end case

Additional information [?]

Size range 411-710 amino acids
Related rules MF_03126 (PURA1 supersedes the current rule); MF_03127 (PURA2 supersedes the current rule)
Fusion None