HAMAP rule MF_03125
General rule information
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Accession | MF_03125 |
Dates | 13-AUG-2010 (Created)
3-SEP-2024 (Last updated, Version 15) |
Name | Adenylosucc_synth_euk |
Scope(s) |
Eukaryota Plastid |
Template(s) | Q9U8D3 (PURA_PLAFA); P0A7D4 (PURA_ECOLI); Q96529 (PURA_ARATH); [ Recover all ] |
Triggered by |
case c? <OC:Eukaryota> or <OG:Plastid>
HAMAP; MF_00011 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | PURA |
case <OG:Organellar chromatophore> | |
Protein name | RecName: Full=Adenylosuccinate synthetase, organellar chromatophore; Short=AMPSase; Short=AdSS; EC=6.3.4.4; AltName: Full=IMP--aspartate ligase; |
else case <OC:Viridiplantae> | |
Protein name | RecName: Full=Adenylosuccinate synthetase, chloroplastic; Short=AMPSase; Short=AdSS; EC=6.3.4.4; AltName: Full=IMP--aspartate ligase; |
else | |
Protein name | RecName: Full=Adenylosuccinate synthetase; Short=AMPSase; Short=AdSS; EC=6.3.4.4; AltName: Full=IMP--aspartate ligase; |
end case | |
case <OC:Viridiplantae> | |
Gene name | Name=PURA; |
else case <OC:Caenorhabditis> | |
Gene name | Name=adss-1; |
else case <OC:Saccharomyces> | |
Gene name | Name=ADE12; |
end case |
Comments
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case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma> | |
FUNCTION | Plays an important role in the salvage pathway for purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
else | |
FUNCTION | Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
end case | |
CATALYTIC ACTIVITY | Reaction=IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+); Xref=Rhea:RHEA:15753, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57567, ChEBI:CHEBI:58053, ChEBI:CHEBI:58189; EC=6.3.4.4; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
PATHWAY | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
SUBUNIT | Homodimer. |
case <OG:Organellar chromatophore> | |
SUBCELLULAR LOCATION | Plastid, organellar chromatophore. |
else case <OC:Viridiplantae> | |
SUBCELLULAR LOCATION | Plastid, chloroplast. |
else | |
SUBCELLULAR LOCATION | Cytoplasm. |
end case | |
case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma> | |
MISCELLANEOUS | Parasitic protozoa lack the de novo purine biosynthesis pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements. |
end case | |
case <OC:Viridiplantae> and not <AnyFeature:TransitC> | |
MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case | |
SIMILARITY | Belongs to the adenylosuccinate synthetase family. |
Keywords
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GTP-binding | |
Ligase | |
Magnesium | |
Metal-binding | |
Nucleotide-binding | |
Purine biosynthesis | |
case <OG:Organellar chromatophore> | |
Organellar chromatophore | |
Plastid | |
else case <OC:Viridiplantae> | |
Chloroplast | |
Plastid | |
else | |
Cytoplasm | |
end case |
Gene Ontology
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GO:0005737; Cellular component:cytoplasm | |
GO:0004019; Molecular function:adenylosuccinate synthase activity | |
GO:0005525; Molecular function:GTP binding | |
GO:0000287; Molecular function:magnesium ion binding | |
GO:0044208; Biological process:'de novo' AMP biosynthetic process | |
case <OG:Organellar chromatophore> | |
GO:0070111; Cellular component:organellar chromatophore | |
else case <OCellular component:Viridiplantae> | |
GO:0009507; Cellular component:chloroplast | |
else; https://www.ebi.ac.uk/QuickGO/term/else | |
GO:0005737; Cellular component:cytoplasm | |
end case |
Cross-references
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PROSITE | PS01266; ADENYLOSUCCIN_SYN_1; 1; |
PROSITE | PS00513; ADENYLOSUCCIN_SYN_2; 1; |
Pfam | PF00709; Adenylsucc_synt; 1; |
NCBIfam | TIGR00184; PurA; 1; |
General | TransitC; -; 0-1; |
Features
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From: PURA_PLAFA (Q9U8D3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 25 | 31 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-D-E-G-K-G-K | ||||||||
BINDING | 53 | 55 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-H-[EST] | ||||||||
BINDING | 339 | 341 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
K-[ILMV]-D | ||||||||
BINDING | 425 | 427 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-[INTV]-G | ||||||||
BINDING | 26 | 29 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
D-E-G-K | ||||||||
BINDING | 51 | 54 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
N-[AS]-G-H | ||||||||
BINDING | 307 | 313 | /ligand="substrate" | x-x-T-x-[KR]-x-R | ||||||||
ACT_SITE | 26 | 26 | /note="Proton acceptor" | D | ||||||||
ACT_SITE | 54 | 54 | /note="Proton donor" | H | ||||||||
BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
BINDING | 53 | 53 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
G | ||||||||
BINDING | 141 | 141 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
T | ||||||||
BINDING | 155 | 155 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners" |
[KR] | ||||||||
BINDING | 232 | 232 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
[QN] | ||||||||
BINDING | 247 | 247 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
T | ||||||||
BINDING | 311 | 311 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
[KR] | ||||||||
BINDING | 313 | 313 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
case <OC:Plasmodium> | ||||||||||||
BINDING | 62 | 62 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
K | ||||||||
BINDING | 307 | 307 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
T | ||||||||
end case |
Additional information
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Size range | 411-710 amino acids |
Related rules |
MF_03126 MF_03127 |
Fusion | Nter: None Cter: None |