HAMAP rule MF_03125
General rule information
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Accession | MF_03125 |
Dates | 13-AUG-2010 (Created) 19-NOV-2022 (Last updated, Version 12) |
Name | Adenylosucc_synth_euk |
Scope | Eukaryota; except Vertebrata
Plastid |
Templates | Q9U8D3 (PURA_PLAFA); P0A7D4 (PURA_ECOLI); Q96529 (PURA_ARATH): [Recover all] |
case <OC:Eukaryota> or <OG:Plastid>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OG:Organellar chromatophore>
Protein name |
|
else case <OC:Viridiplantae>
Protein name |
|
else
Protein name |
|
end case
case <OC:Viridiplantae>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
else case <OC:Saccharomyces>
Gene name |
|
end case
Comments
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case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma>
Function | Plays an important role in the salvage pathway for purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
else
Function | Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
end case
Catalytic activity | RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4 |
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
Subunit | Homodimer. |
case <OG:Organellar chromatophore>
Subcellular location | Plastid, organellar chromatophore. |
else case <OC:Viridiplantae>
Subcellular location | Plastid, chloroplast. |
else
Subcellular location | Cytoplasm. |
end case
case <OC:Apicomplexa> or <OC:Leishmania> or <OC:Trypanosoma>
Miscellaneous | Parasitic protozoa lack the de novo purine biosynthesis pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements. |
end case
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity | Belongs to the adenylosuccinate synthetase family. |
Keywords
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case <OG:Organellar chromatophore>
else case <OC:Viridiplantae>
else
end case
Gene Ontology
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GO:0005737; Cellular component: cytoplasm.
GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0006167; Biological process: AMP biosynthetic process.
GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0006167; Biological process: AMP biosynthetic process.
case <OG:Organellar chromatophore>
GO:0070111; Cellular component: organellar chromatophore.
else case <OC:Viridiplantae>
GO:0009507; Cellular component: chloroplast.
else
GO:0005737; Cellular component: cytoplasm.
end case
Cross-references
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PROSITE | PS01266; ADENYLOSUCCIN_SYN_1; 1; |
PS00513; ADENYLOSUCCIN_SYN_2; 1; | |
Pfam | PF00709; Adenylsucc_synt; 1; |
TIGRFAMs | TIGR00184; PurA; 1; |
Computed features
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case <OC:Viridiplantae>
General | TransitC; -; 0-1; trigger=yes; |
end case
Features
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From: PURA_PLAFA (Q9U8D3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 25 | 31 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-D-E-G-K-G-K | ||||||||
BINDING | 53 | 55 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-H-[EST] | ||||||||
BINDING | 339 | 341 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | K-[ILMV]-D | ||||||||
BINDING | 425 | 427 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-[INTV]-G | ||||||||
BINDING | 26 | 29 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | D-E-G-K | ||||||||
BINDING | 51 | 54 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | N-[AS]-G-H | ||||||||
BINDING | 307 | 313 | /ligand="substrate | x-x-T-x-[KR]-x-R | ||||||||
ACT_SITE | 26 | 26 | Proton acceptor | D | ||||||||
ACT_SITE | 54 | 54 | Proton donor | H | ||||||||
BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 53 | 53 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | G | ||||||||
BINDING | 141 | 141 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | T | ||||||||
BINDING | 155 | 155 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners | [KR] | ||||||||
BINDING | 232 | 232 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | [QN] | ||||||||
BINDING | 247 | 247 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | T | ||||||||
BINDING | 311 | 311 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | [KR] | ||||||||
BINDING | 313 | 313 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | R |
case <OC:Plasmodium>
BINDING | 62 | 62 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | K | ||||||||
BINDING | 307 | 307 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | T |
end case
Additional information
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Size range | 411-710 amino acids |
Related rules | MF_03126 (PURA1 supersedes the current rule); MF_03127 (PURA2 supersedes the current rule) |
Fusion | None |