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HAMAP rule MF_03130

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General rule information [?]

Accession MF_03130
Dates 22-OCT-2010 (Created)
19-NOV-2022 (Last updated, Version 14)
Name mec17
Scope(s) Eukaryota
Template(s) Q5SQI0 (ATAT_HUMAN); Q8K341 (ATAT_MOUSE); Q6PH17 (ATAT_DANRE); Q22XZ3 (ATAT_TETTS); O45100 (ATAT1_CAEEL); [ Recover all ]
Triggered by HAMAP; MF_03130 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ATAT
case <OC:Vertebrata>
Protein name RecName: Full=Alpha-tubulin N-acetyltransferase 1;
                 Short=Alpha-TAT;
                 Short=Alpha-TAT1;
                 Short=TAT;
                 EC=2.3.1.108;
AltName: Full=Acetyltransferase mec-17 homolog;
else case <OC:Caenorhabditis>
Protein name RecName: Full=Alpha-tubulin N-acetyltransferase;
                 Short=Alpha-TAT;
                 Short=TAT;
                 EC=2.3.1.108;
else
Protein name RecName: Full=Alpha-tubulin N-acetyltransferase;
                 Short=Alpha-TAT;
                 Short=TAT;
                 EC=2.3.1.108;
AltName: Full=Acetyltransferase mec-17 homolog;
end case
case <OC:Caenorhabditis>
Gene name Name=mec-17;
else case <OC:Vertebrata>
Gene name Name=ATAT1; Synonyms=MEC17;
end case

Comments [?]

case <OC:Mammalia>
FUNCTIONSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
else case <OC:Vertebrata>
FUNCTIONSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. May be involved in neuron development.
else case <OC:Caenorhabditis>
FUNCTIONSpecifically acetylates 'Lys-40' in alpha-tubulin/mec-12 on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for the maintenance of touch receptor neurons and possibly other type of neurons involved in locomotion.
else
FUNCTIONSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.
end case
CATALYTIC ACTIVITY Reaction=acetyl-CoA + L-lysyl-[alpha-tubulin] = CoA + H(+) + N(6)- acetyl-L-lysyl-[alpha-tubulin]; Xref=Rhea:RHEA:15277, Rhea:RHEA- COMP:11278, Rhea:RHEA-COMP:11279, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:61930; EC=2.3.1.108;
case <OC:Mammalia>
SUBUNITComponent of the BBSome complex. Interacts with AP2 alpha- adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.
PTMAutoacetylation strongly increases tubulin acetylation.
end case
case <OC:Vertebrata>
SUBCELLULAR LOCATIONCytoplasm. Membrane, clathrin-coated pit. Cell junction, focal adhesion. Cell projection, axon. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle.
end case
SIMILARITYBelongs to the acetyltransferase ATAT1 family.

Keywords [?]

case <OC:Mammalia>
Acetylation
end case
case <OC:Vertebrata>
Cell junction
Cell projection
Coated pit
Cytoplasm
Cytoskeleton
Membrane
end case
Acyltransferase
Transferase

Gene Ontology [?]

GO:0019799; Molecular function:tubulin N-acetyltransferase activity
GO:0071929; Biological process:alpha-tubulin acetylation
GO:0070507; Biological process:regulation of microtubule cytoskeleton organization
case <OCellular component:Metazoa> and not <OC:Placozoa> and not <OC:Porifera>
GO:0048666; Biological process:neuron development
end case

Cross-references [?]

Pfam PF05301; Mec-17; 1;
PROSITE PS51730; GNAT_ATAT; 1;

Features [?]

From: ATAT_HUMAN (Q5SQI0)
Key From To Description Tag Condition FTGroup
BINDING 124 137 /ligand="acetyl-CoA"
/ligand_id="ChEBI:CHEBI:57288"		 F-[YF]-x(5)-[QR]-[RK]-x-G-x-G-x
BINDING 160 169 /ligand="acetyl-CoA"
/ligand_id="ChEBI:CHEBI:57288"		 S-x-K-[FLM]-x(2)-F-[LM]-x-K
SITE 58 58 /note="Crucial for catalytic activity" Q
case <OC:Mammalia>
MOD_RES 56 56 /note="N6-acetyllysine; by autocatalysis" K
MOD_RES 146 146 /note="N6-acetyllysine; by autocatalysis" K
MOD_RES 233 233 /note="N6-acetyllysine; by autocatalysis" K
MOD_RES 244 244 /note="N6-acetyllysine; by autocatalysis" K
end case

Additional information [?]

Size range 179-483 amino acids
Related rules None
Fusion Nter: None Cter: None



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