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HAMAP rule MF_03133

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General rule information [?]

Accession MF_03133
Dates 22-OCT-2010 (Created)
19-NOV-2022 (Last updated, Version 11)
Name Ala_tRNA_synth_euk
Scope
Eukaryota
Plastid
Templates P49588 (SYAC_HUMAN); P40825 (SYA_YEAST); P36428 (SYA_ARATH); Q5A8K2 (SYA_CANAL): [Recover all]
case <OC:Eukaryota> or <OG:Plastid>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Identifier
SYAC
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Identifier
SYAM
else
Identifier
SYA
end case
case <OG:Organellar chromatophore>
Protein name
RecName: Full=Alanine--tRNA ligase, organellar chromatophore;
EC 6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
Short=AlaRS;
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Protein name
RecName: Full=Alanine--tRNA ligase, cytoplasmic;
EC 6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
Short=AlaRS;
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Protein name
RecName: Full=Alanine--tRNA ligase, mitochondrial;
EC 6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
Short=AlaRS;
else
Protein name
RecName: Full=Alanine--tRNA ligase;
EC 6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
Short=AlaRS;
end case
case <OC:Vertebrata>
Gene name
AARS
else case <OC:Drosophilidae> and <AnyFeature:TransitM>
Gene name
AlaRS-m
else case <OC:Drosophilidae> and not <AnyFeature:TransitM>
Gene name
AlaRS
else case <OC:Caenorhabditis>
Gene name
aars
else case <OC:Fungi>
Gene name
ALA1
end case

Comments [?]

Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic activity RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
EC 6.1.1.7
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
case <OC:Vertebrata>
Subunit Monomer. Interacts with ANKRD16; the interaction is direct.
else
Subunit Monomer.
end case
case <OG:Organellar chromatophore>
Subcellular location Plastid, organellar chromatophore.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Subcellular location Cytoplasm.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Subcellular location Mitochondrion.
else
Subcellular location Mitochondrion. Cytoplasm.
end case
Domain Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
case <OC:Mammalia>
Ptm ISGylated.
end case
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
case <FTGroup:1>
Metal-binding
Zinc
end case
case <OG:Organellar chromatophore>
Organellar chromatophore
Plastid
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Cytoplasm
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Mitochondrion
else
Cytoplasm
Mitochondrion
end case
case <FTTag:acetyl>
Acetylation
end case
case <OC:Mammalia>
Ubl conjugation
end case

Gene Ontology [?]

GO:0004813; Molecular function: alanine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
case <OG:Organellar chromatophore>
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0070111; Cellular component: organellar chromatophore.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
GO:0070143; Biological process: mitochondrial alanyl-tRNA aminoacylation.
GO:0005739; Cellular component: mitochondrion.
else
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0070143; Biological process: mitochondrial alanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005739; Cellular component: mitochondrion.
end case

Cross-references [?]

Pfam PF02272; DHHA1; 1;
PF01411; tRNA-synt_2c; 1;
PF07973; tRNA_SAD; 1;
PRINTS PR00980; TRNASYNTHALA; 1;
TIGRFAMs TIGR00344; AlaS; 1;
PROSITE PS50860; AA_TRNA_LIGASE_II_ALA; 1;

Computed features [?]

case (<OC:Metazoa> or <OC:Amoebozoa>)
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

From: SYAC_HUMAN (P49588)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     605     605       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H   1
BINDING     609     609       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H   1
BINDING     723     723       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING     727     727       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H   1
case <OC:Mammalia>
MOD_RES     1     1       N-acetylmethionine   acetyl   M  
end case

Additional information [?]

Size range 433-1408 amino acids
Related rules MF_03134 (SYAP supersedes the current rule)
Fusion None


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