HAMAP rule MF_03133
General rule information
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Accession | MF_03133 |
Dates | 22-OCT-2010 (Created) 19-NOV-2022 (Last updated, Version 11) |
Name | Ala_tRNA_synth_euk |
Scope | Eukaryota
Plastid |
Templates | P49588 (SYAC_HUMAN); P40825 (SYA_YEAST); P36428 (SYA_ARATH); Q5A8K2 (SYA_CANAL): [Recover all] |
case <OC:Eukaryota> or <OG:Plastid>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Identifier |
|
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Identifier |
|
else
Identifier |
|
end case
case <OG:Organellar chromatophore>
Protein name |
|
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Protein name |
|
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Protein name |
|
else
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Drosophilidae> and <AnyFeature:TransitM>
Gene name |
|
else case <OC:Drosophilidae> and not <AnyFeature:TransitM>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
else case <OC:Fungi>
Gene name |
|
end case
Comments
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Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
Catalytic activity | RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
EC 6.1.1.7 |
case <FTGroup:1>
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
end case
case <OC:Vertebrata>
Subunit | Monomer. Interacts with ANKRD16; the interaction is direct. |
else
Subunit | Monomer. |
end case
case <OG:Organellar chromatophore>
Subcellular location | Plastid, organellar chromatophore. |
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Subcellular location | Cytoplasm. |
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Subcellular location | Mitochondrion. |
else
Subcellular location | Mitochondrion. Cytoplasm. |
end case
Domain | Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. |
case <OC:Mammalia>
Ptm | ISGylated. |
end case
Similarity | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
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Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
case <FTGroup:1>
end case
case <OG:Organellar chromatophore>
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
else
end case
case <FTTag:acetyl>
end case
case <OC:Mammalia>
end case
Gene Ontology
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GO:0004813; Molecular function: alanine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
case <OG:Organellar chromatophore>
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0070111; Cellular component: organellar chromatophore.
GO:0070111; Cellular component: organellar chromatophore.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005737; Cellular component: cytoplasm.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
GO:0070143; Biological process: mitochondrial alanyl-tRNA aminoacylation.
GO:0005739; Cellular component: mitochondrion.
GO:0005739; Cellular component: mitochondrion.
else
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0070143; Biological process: mitochondrial alanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005739; Cellular component: mitochondrion.
GO:0070143; Biological process: mitochondrial alanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005739; Cellular component: mitochondrion.
end case
Cross-references
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Pfam | PF02272; DHHA1; 1; |
PF01411; tRNA-synt_2c; 1; | |
PF07973; tRNA_SAD; 1; | |
PRINTS | PR00980; TRNASYNTHALA; 1; |
TIGRFAMs | TIGR00344; AlaS; 1; |
PROSITE | PS50860; AA_TRNA_LIGASE_II_ALA; 1; |
Computed features
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case (<OC:Metazoa> or <OC:Amoebozoa>)
General | TransitM; -; 0-1; trigger=yes; |
end case
Features
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From: SYAC_HUMAN (P49588) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 605 | 605 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 609 | 609 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 723 | 723 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 727 | 727 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 |
case <OC:Mammalia>
end case
Additional information
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Size range | 433-1408 amino acids |
Related rules | MF_03134 (SYAP supersedes the current rule) |
Fusion | None |