HAMAP rule MF_03152
General rule information
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Accession | MF_03152 |
Dates | 17-OCT-2011 (Created) 19-NOV-2022 (Last updated, Version 10) |
Name | TRM5 |
Scope | Eukaryota |
Templates | P38793 (TRM5_YEAST); Q32P41 (TRM5_HUMAN): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Saccharomyces>
Protein name |
|
else case <OC:Fungi>
Protein name |
|
else
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Fungi>
Gene name |
|
end case
Comments
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case <OC:Saccharomyces>
Function | Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes. |
else
Function | Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. |
end case
Catalytic activity | RHEA:36899: guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine
EC 2.1.1.228 |
Subunit | Monomer. |
Subcellular location | Mitochondrion matrix. Nucleus. Cytoplasm. Note=Predominantly in the mitochondria and in the nucleus. |
Similarity | Belongs to the TRM5 / TYW2 family. |
Keywords
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Cytoplasm
Methyltransferase
Mitochondrion
Nucleus
S-adenosyl-L-methionine
Transferase
tRNA processing
Methyltransferase
Mitochondrion
Nucleus
S-adenosyl-L-methionine
Transferase
tRNA processing
case <OC:Saccharomyces>
end case
Gene Ontology
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GO:0005759; Cellular component: mitochondrial matrix.
GO:0005634; Cellular component: nucleus.
GO:0005737; Cellular component: cytoplasm.
GO:0030488; Biological process: tRNA methylation.
GO:0009019; Molecular function: tRNA (guanine-N1-)-methyltransferase activity.
GO:0005634; Cellular component: nucleus.
GO:0005737; Cellular component: cytoplasm.
GO:0030488; Biological process: tRNA methylation.
GO:0009019; Molecular function: tRNA (guanine-N1-)-methyltransferase activity.
Cross-references
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Computed features
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case not <OC:Saccharomyces>
General | TransitM; -; 0-1; trigger=yes; |
end case
Features
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case <OC:Saccharomyces>
From: TRM5_YEAST (P38793) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
TRANSIT | Nter | 44 | Mitochondrion | |||||||||
CHAIN | 45 | Cter |
|
end case
Additional information
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Size range | 360-781 amino acids |
Related rules | None |
Fusion | None |