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HAMAP rule MF_03152

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General rule information [?]

Accession MF_03152
Dates 17-OCT-2011 (Created)
19-NOV-2022 (Last updated, Version 10)
Name TRM5
Scope
Eukaryota
Templates P38793 (TRM5_YEAST); Q32P41 (TRM5_HUMAN): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRM5
case <OC:Saccharomyces>
Protein name
RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
EC 2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5;
Flags: Precursor;
else case <OC:Fungi>
Protein name
RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
EC 2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5;
else
Protein name
RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
EC 2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5 homolog;
end case
case <OC:Vertebrata>
Gene name
TRMT5, TRM5
else case <OC:Fungi>
Gene name
TRM5
end case

Comments [?]

case <OC:Saccharomyces>
Function Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.
else
Function Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
end case
Catalytic activity RHEA:36899: guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine
EC 2.1.1.228
Subunit Monomer.
Subcellular location Mitochondrion matrix. Nucleus. Cytoplasm. Note=Predominantly in the mitochondria and in the nucleus.
Similarity Belongs to the TRM5 / TYW2 family.

Keywords [?]

Cytoplasm
Methyltransferase
Mitochondrion
Nucleus
S-adenosyl-L-methionine
Transferase
tRNA processing
case <OC:Saccharomyces>
Transit peptide
end case

Gene Ontology [?]

GO:0005759; Cellular component: mitochondrial matrix.
GO:0005634; Cellular component: nucleus.
GO:0005737; Cellular component: cytoplasm.
GO:0030488; Biological process: tRNA methylation.
GO:0009019; Molecular function: tRNA (guanine-N1-)-methyltransferase activity.

Cross-references [?]

PROSITE PS51684; SAM_MT_TRM5_TYW2; 1;
Pfam PF02475; Met_10; 1;

Computed features [?]

case not <OC:Saccharomyces>
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

case <OC:Saccharomyces>
From: TRM5_YEAST (P38793)
Key     From     To       Description   Tag   Condition   FTGroup
TRANSIT     Nter     44       Mitochondrion        
CHAIN     45     Cter              
end case
BINDING     307     308       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [DE]-[ILM]  
BINDING     335     336       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [DE]-[AGNSV]  
BINDING     268     268       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [HKR]  
BINDING     399     399       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     N  

Additional information [?]

Size range 360-781 amino acids
Related rules None
Fusion None


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