HAMAP rule MF_03154
General rule information
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Accession | MF_03154 |
Dates | 27-OCT-2011 (Created) 19-NOV-2022 (Last updated, Version 13) |
Name | Salvage_MtnD_euk |
Scope | Eukaryota |
Templates | Q99JT9 (MTND_MOUSE); Q03677 (MTND_YEAST); Q9BV57 (MTND_HUMAN): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <FTGroup:1>
Protein name |
|
else
Protein name |
|
end case
case <OC:Vertebrata>
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Fungi> or <OC:Dictyostelia>
Gene name |
|
end case
Comments
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case <FTGroup:1> and <OC:Mammalia>
Function | Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14. |
else case <FTGroup:1> and not <OC:Mammalia>
Function | Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. |
end case
case <FTGroup:1>
Catalytic activity | RHEA:24504: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)
EC 1.13.11.54 |
RHEA:14161: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)
EC 1.13.11.53 |
|
Cofactor | Fe(2+) Ni(2+) Note: Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate. |
Pathway | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. |
end case
case <OC:Mammalia>
Subunit | Monomer. Interacts with MMP14. |
Subcellular location | Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to the plasma membrane when complexed to MMP14. |
else case <OC:Vertebrata> and not <OC:Mammalia>
Subunit | Monomer. |
Subcellular location | Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
else
Subcellular location | Cytoplasm. Nucleus. |
end case
Similarity | Belongs to the acireductone dioxygenase (ARD) family. |
case not <FTGroup:1>
Function | Probable inactive acireductone dioxygenase. |
Caution | This enzyme lacks one or more conserved metal-binding sites. It may be non-functional. |
end case
Keywords
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case <FTGroup:1>
Amino-acid biosynthesis
Dioxygenase
Methionine biosynthesis
Oxidoreductase
Metal-binding
Iron
Nickel
Dioxygenase
Methionine biosynthesis
Oxidoreductase
Metal-binding
Iron
Nickel
end case
case <OC:Vertebrata>
end case
Gene Ontology
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case <FTGroup:1>
GO:0005506; Molecular function: iron ion binding.
GO:0016151; Molecular function: nickel cation binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0019509; Biological process: L-methionine salvage from methylthioadenosine.
GO:0016151; Molecular function: nickel cation binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0019509; Biological process: L-methionine salvage from methylthioadenosine.
end case
Cross-references
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Pfam | PF03079; ARD; 1; |
Features
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From: MTND_YEAST (Q03677) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 85 | 85 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" /ligand_note="for iron-dependent acireductone dioxygenase activity | H | 1 | |||||||
BINDING | 85 | 85 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_note="for nickel-dependent acireductone dioxygenase activity | H | 1 | |||||||
BINDING | 87 | 87 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" /ligand_note="for iron-dependent acireductone dioxygenase activity | H | 1 | |||||||
BINDING | 87 | 87 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_note="for nickel-dependent acireductone dioxygenase activity | H | 1 | |||||||
BINDING | 91 | 91 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" /ligand_note="for iron-dependent acireductone dioxygenase activity | E | 1 | |||||||
BINDING | 91 | 91 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_note="for nickel-dependent acireductone dioxygenase activity | E | 1 | |||||||
BINDING | 132 | 132 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" /ligand_note="for iron-dependent acireductone dioxygenase activity | H | 1 | |||||||
BINDING | 132 | 132 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_note="for nickel-dependent acireductone dioxygenase activity | H | 1 |
case <OC:Mammalia>
From: MTND_HUMAN (Q03677) | ||||||||||||
INIT_MET | 1 | 1 | Removed | M |
end case
Additional information
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Size range | 146-344 amino acids |
Related rules | None |
Fusion | None |