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Annotation rule MF_03156
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General rule information [?]

Accession MF_03156
Dates 5-JAN-2012 (Created)
11-FEB-2020 (Last updated, Version 10)
Name IMPDH_euk
Scope
Eukaryota
Templates P50097 (IMDH_TRIFO); P20839 (IMDH1_HUMAN); P21620 (IMDH_LEIDO): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
IMDH
Protein name
RecName: Full=Inosine-5'-monophosphate dehydrogenase;
Short=IMP dehydrogenase;
Short=IMPD;
Short=IMPDH;
EC 1.1.1.205;
case <OC:Vertebrata>
Gene name
IMPDH
else case <OC:Saccharomyces>
Gene name
IMD
end case

Comments [?]

case <OC:Mammalia>
Function Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
else
Function Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.
end case
Catalytic activity RHEA:11708: H2O + IMP + NAD(+) = H(+) + NADH + XMP
EC 1.1.1.205
Cofactor K(+)
Activity regulation Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.
Pathway Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Subunit Homotetramer.
case <OC:Vertebrata>
Subcellular location Cytoplasm. Nucleus.
else case <OC:Kinetoplastea> and <FTTag:Microbody>
Subcellular location Glycosome.
else
Subcellular location Cytoplasm.
end case
Similarity Belongs to the IMPDH/GMPR family.

Keywords [?]

case <OC:Vertebrata>
Nucleus
end case
case <OC:Kinetoplastea> and <FTTag:Microbody>
Glycosome
Peroxisome
else
Cytoplasm
end case
GMP biosynthesis
Metal-binding
NAD
Oxidoreductase
Potassium
Purine biosynthesis

Gene Ontology [?]

case <OC:Vertebrata>
GO:0005634; Cellular component: nucleus.
end case
GO:0005737; Cellular component: cytoplasm.
GO:0003938; Molecular function: IMP dehydrogenase activity.
GO:0046872; Molecular function: metal ion binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0006177; Biological process: GMP biosynthetic process.

Cross-references [?]

PROSITE PS51371; CBS; 0-2; trigger=PRU00703;
PS00487; IMP_DH_GMP_RED; 1;
Pfam PF00571; CBS; 2;
PF00478; IMPDH; 1;
TIGRFAMs TIGR01302; IMP_dehydrog; 1;
PIRSF PIRSF000130; IMPDH; 1;

Features [?]

case <OC:Mammalia>
From: IMDH1_HUMAN (P20839)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     1     1       Removed     M  
end case
From: IMDH_TRIFO (P50097)
NP_BIND (Optional)     261     263       NAD     D-S-S  
case not <FT:2=D-S-S>
BINDING (Optional)     261     261       NAD     D  
end case
NP_BIND     312     314       NAD     G-x-G  
REGION     358     360       IMP binding     D-G-G  
REGION     381     382       IMP binding     G-x  
REGION     405     409       IMP binding     [YI]-x-x-x-[GA]  
ACT_SITE     319     319       Thioimidate intermediate     C  
ACT_SITE     418     418       Proton acceptor     R  
METAL     314     314       Potassium; via carbonyl oxygen     G  
METAL     316     316       Potassium; via carbonyl oxygen     G  
METAL     319     319       Potassium; via carbonyl oxygen     C  
METAL (Optional)     485     485       Potassium; via carbonyl oxygen; shared with tetrameric partner     E  
METAL (Optional)     486     486       Potassium; via carbonyl oxygen; shared with tetrameric partner     [GS]  
METAL (Optional)     487     487       Potassium; via carbonyl oxygen; shared with tetrameric partner     [GH]  
BINDING     317     317       IMP     S  
BINDING     431     431       IMP     [EQ]  
case <OC:Kinetoplastea>
From: IMDH_LEIDO (P21620)
MOTIF     512     Cter       Microbody targeting signal   Microbody   [AS]-K-[ML]  
end case

Additional information [?]

Size range 400-554 amino acids
Related rules None
Fusion None


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