HAMAP rule MF_03157
General rule information
[?]
Accession | MF_03157 |
Dates | 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 14) |
Name | NADHX_dehydratase_euk |
Scope(s) |
Eukaryota |
Template(s) | P36059 (NNRD_YEAST); Q9CZ42 (NNRD_MOUSE); P94368 (NNRD_BACSU); [ Recover all ] |
Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_01965 (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier | NNRD |
case <OC:Mammalia> | |
Protein name | RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase; EC=4.2.1.93; AltName: Full=ATP-dependent NAD(P)HX dehydratase; AltName: Full=Carbohydrate kinase domain-containing protein; |
Gene name | Name=CARKD; |
else | |
Protein name | RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase; EC=4.2.1.93; AltName: Full=ATP-dependent NAD(P)HX dehydratase; |
end case |
Comments
[?]
FUNCTION | Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. |
CATALYTIC ACTIVITY | Reaction=(6S)-NADHX + ATP = ADP + H(+) + NADH + phosphate; Xref=Rhea:RHEA:19017, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57945, ChEBI:CHEBI:64074, ChEBI:CHEBI:456216; EC=4.2.1.93; |
CATALYTIC ACTIVITY | Reaction=(6S)-NADPHX + ATP = ADP + H(+) + NADPH + phosphate; Xref=Rhea:RHEA:32231, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57783, ChEBI:CHEBI:64076, ChEBI:CHEBI:456216; EC=4.2.1.93; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
case <OC:Fungi> | |
SUBCELLULAR LOCATION | Cytoplasm. |
else case <OC:Mammalia> | |
SUBCELLULAR LOCATION | Mitochondrion. |
end case | |
case <OC:Mammalia> and not <AnyFeature:TransitM> | |
MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case | |
SIMILARITY | Belongs to the NnrD/CARKD family. |
Keywords
[?]
ATP-binding | |
Lyase | |
NAD | |
Nucleotide-binding | |
Phosphoprotein | |
case <OC:Fungi> | |
Cytoplasm | |
else case <OC:Mammalia> | |
Mitochondrion | |
end case |
Gene Ontology
[?]
GO:0047453; Molecular function:ATP-dependent NAD(P)H-hydrate dehydratase activity | |
GO:0046496; Biological process:nicotinamide nucleotide metabolic process | |
case <OCellular component:Fungi> | |
GO:0005737; Cellular component:cytoplasm | |
else case <OCellular component:Mammalia> | |
GO:0005739; Cellular component:mitochondrion | |
end case |
Cross-references
[?]
PROSITE | PS01049; YJEF_C_1; 1; |
PROSITE | PS01050; YJEF_C_2; 1; |
PROSITE | PS51383; YJEF_C_3; 1; |
Pfam | PF01256; Carb_kinase; 1; |
NCBIfam | TIGR00196; YjeF_C; 1; |
General | TransitM; -; 0-1; |
Features
[?]
From: NNRD_BACSU (P94368) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 186 | 190 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K-x(4) | ||||||||
BINDING | 206 | 215 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
x(7)-G-x-G | ||||||||
BINDING | 149 | 155 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076" |
x(3)-E-x(3) | ||||||||
BINDING | 104 | 104 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076" |
|||||||||
BINDING | 216 | 216 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076" |
D |
Additional information
[?]
Size range | 295-413 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |