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HAMAP rule MF_03157

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General rule information [?]

Accession MF_03157
Dates 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 14)
Name NADHX_dehydratase_euk
Scope
Eukaryota
Templates P36059 (NNRD_YEAST); Q9CZ42 (NNRD_MOUSE); P94368 (NNRD_BACSU): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NNRD
case <OC:Mammalia>
Protein name
RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase;
EC 4.2.1.93;
AltName: Full=ATP-dependent NAD(P)HX dehydratase;
AltName: Full=Carbohydrate kinase domain-containing protein;
Gene name
CARKD
else
Protein name
RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase;
EC 4.2.1.93;
AltName: Full=ATP-dependent NAD(P)HX dehydratase;
end case

Comments [?]

Function Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalytic activity RHEA:19017: (6S)-NADHX + ATP = ADP + H(+) + NADH + phosphate
EC 4.2.1.93
RHEA:32231: (6S)-NADPHX + ATP = ADP + H(+) + NADPH + phosphate
EC 4.2.1.93
Cofactor Mg(2+)
case <OC:Fungi>
Subcellular location Cytoplasm.
else case <OC:Mammalia>
Subcellular location Mitochondrion.
end case
case <OC:Mammalia> and not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the NnrD/CARKD family.

Keywords [?]

ATP-binding
Lyase
NAD
Nucleotide-binding
Phosphoprotein
case <OC:Fungi>
Cytoplasm
else case <OC:Mammalia>
Mitochondrion
end case

Gene Ontology [?]

GO:0047453; Molecular function: ATP-dependent NAD(P)H-hydrate dehydratase activity.
GO:0046496; Biological process: nicotinamide nucleotide metabolic process.
case <OC:Fungi>
GO:0005737; Cellular component: cytoplasm.
else case <OC:Mammalia>
GO:0005739; Cellular component: mitochondrion.
end case

Cross-references [?]

PROSITE PS01049; YJEF_C_1; 1;
PS01050; YJEF_C_2; 1;
PS51383; YJEF_C_3; 1; trigger=PRU00715;
Pfam PF01256; Carb_kinase; 1;
NCBIfam TIGR00196; YjeF_C; 1;

Computed features [?]

case <OC:Mammalia>
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

From: NNRD_BACSU (P94368)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     186     190       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K-x(4)  
BINDING     206     215       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     x(7)-G-x-G  
BINDING     149     155       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076     x(3)-E-x(3)  
BINDING     104     104       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076        
BINDING     216     216       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076     D  

Additional information [?]

Size range 295-413 amino acids
Related rules None
Fusion None


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