HAMAP rule MF_03157

General rule information [?]

Accession	MF_03157
Dates	14-FEB-2012 (Created) 1-JUN-2023 (Last updated, Version 14)
Name	NADHX_dehydratase_euk
Scope(s)	Eukaryota
Template(s)	P36059 (NNRD_YEAST); Q9CZ42 (NNRD_MOUSE); P94368 (NNRD_BACSU); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_01965 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	NNRD
case <OC:Mammalia>
Protein name	RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase; EC=4.2.1.93; AltName: Full=ATP-dependent NAD(P)HX dehydratase; AltName: Full=Carbohydrate kinase domain-containing protein;
Gene name	Name=CARKD;
else
Protein name	RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase; EC=4.2.1.93; AltName: Full=ATP-dependent NAD(P)HX dehydratase;
end case

Comments [?]

FUNCTION	Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
CATALYTIC ACTIVITY	Reaction=(6S)-NADHX + ATP = ADP + H(+) + NADH + phosphate; Xref=Rhea:RHEA:19017, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57945, ChEBI:CHEBI:64074, ChEBI:CHEBI:456216; EC=4.2.1.93;
CATALYTIC ACTIVITY	Reaction=(6S)-NADPHX + ATP = ADP + H(+) + NADPH + phosphate; Xref=Rhea:RHEA:32231, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57783, ChEBI:CHEBI:64076, ChEBI:CHEBI:456216; EC=4.2.1.93;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
case <OC:Fungi>
SUBCELLULAR LOCATION	Cytoplasm.
else case <OC:Mammalia>
SUBCELLULAR LOCATION	Mitochondrion.
end case
case <OC:Mammalia> and not <AnyFeature:TransitM>
MISCELLANEOUS	This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITY	Belongs to the NnrD/CARKD family.

Keywords [?]

Gene Ontology [?]

GO:0047453; Molecular function:ATP-dependent NAD(P)H-hydrate dehydratase activity
GO:0046496; Biological process:nicotinamide nucleotide metabolic process
case <OCellular component:Fungi>
GO:0005737; Cellular component:cytoplasm
else case <OCellular component:Mammalia>
GO:0005739; Cellular component:mitochondrion
end case

Cross-references [?]

Features [?]

From: NNRD_BACSU (P94368)

Key

From

Description

Tag

Condition

FTGroup

BINDING

186

190

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

K-x(4)

BINDING

206

215

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

x(7)-G-x-G

BINDING

149

155

/ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"

x(3)-E-x(3)

BINDING

104

/ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"

BINDING

216

/ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"

Additional information [?]