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HAMAP rule MF_03157

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General rule information [?]

Accession MF_03157
Dates 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 14)
Name NADHX_dehydratase_euk
Scope(s) Eukaryota
Template(s) P36059 (NNRD_YEAST); Q9CZ42 (NNRD_MOUSE); P94368 (NNRD_BACSU); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01965 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NNRD
case <OC:Mammalia>
Protein name RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase;
AltName: Full=ATP-dependent NAD(P)HX dehydratase;
AltName: Full=Carbohydrate kinase domain-containing protein;
Gene name Name=CARKD;
Protein name RecName: Full=ATP-dependent (S)-NAD(P)H-hydrate dehydratase;
AltName: Full=ATP-dependent NAD(P)HX dehydratase;
end case

Comments [?]

FUNCTIONCatalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
CATALYTIC ACTIVITY Reaction=(6S)-NADHX + ATP = ADP + H(+) + NADH + phosphate; Xref=Rhea:RHEA:19017, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57945, ChEBI:CHEBI:64074, ChEBI:CHEBI:456216; EC=;
CATALYTIC ACTIVITY Reaction=(6S)-NADPHX + ATP = ADP + H(+) + NADPH + phosphate; Xref=Rhea:RHEA:32231, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57783, ChEBI:CHEBI:64076, ChEBI:CHEBI:456216; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
case <OC:Fungi>
else case <OC:Mammalia>
end case
case <OC:Mammalia> and not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the NnrD/CARKD family.

Keywords [?]

case <OC:Fungi>
else case <OC:Mammalia>
end case

Gene Ontology [?]

GO:0047453; Molecular function:ATP-dependent NAD(P)H-hydrate dehydratase activity
GO:0046496; Biological process:nicotinamide nucleotide metabolic process
case <OCellular component:Fungi>
GO:0005737; Cellular component:cytoplasm
else case <OCellular component:Mammalia>
GO:0005739; Cellular component:mitochondrion
end case

Cross-references [?]

PROSITE PS01049; YJEF_C_1; 1;
PROSITE PS01050; YJEF_C_2; 1;
PROSITE PS51383; YJEF_C_3; 1;
Pfam PF01256; Carb_kinase; 1;
NCBIfam TIGR00196; YjeF_C; 1;
General TransitM; -; 0-1;

Features [?]

From: NNRD_BACSU (P94368)
Key From To Description Tag Condition FTGroup
BINDING 186 190 /ligand="ATP"
BINDING 206 215 /ligand="ATP"
BINDING 149 155 /ligand="(6S)-NADPHX"
BINDING 104 104 /ligand="(6S)-NADPHX"
BINDING 216 216 /ligand="(6S)-NADPHX"

Additional information [?]

Size range 295-413 amino acids
Related rules None
Fusion Nter: None Cter: None

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