HAMAP rule MF_03157
General rule information
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Accession | MF_03157 |
Dates | 14-FEB-2012 (Created) 1-JUN-2023 (Last updated, Version 14) |
Name | NADHX_dehydratase_euk |
Scope | Eukaryota |
Templates | P36059 (NNRD_YEAST); Q9CZ42 (NNRD_MOUSE); P94368 (NNRD_BACSU): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Mammalia>
Protein name |
|
Gene name |
|
else
Protein name |
|
end case
Comments
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Function | Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. |
Catalytic activity | RHEA:19017: (6S)-NADHX + ATP = ADP + H(+) + NADH + phosphate
EC 4.2.1.93 |
RHEA:32231: (6S)-NADPHX + ATP = ADP + H(+) + NADPH + phosphate
EC 4.2.1.93 |
|
Cofactor | Mg(2+) |
case <OC:Fungi>
Subcellular location | Cytoplasm. |
else case <OC:Mammalia>
Subcellular location | Mitochondrion. |
end case
case <OC:Mammalia> and not <AnyFeature:TransitM>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity | Belongs to the NnrD/CARKD family. |
Keywords
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case <OC:Fungi>
else case <OC:Mammalia>
end case
Gene Ontology
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GO:0047453; Molecular function: ATP-dependent NAD(P)H-hydrate dehydratase activity.
GO:0046496; Biological process: nicotinamide nucleotide metabolic process.
GO:0046496; Biological process: nicotinamide nucleotide metabolic process.
case <OC:Fungi>
GO:0005737; Cellular component: cytoplasm.
else case <OC:Mammalia>
GO:0005739; Cellular component: mitochondrion.
end case
Cross-references
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PROSITE | PS01049; YJEF_C_1; 1; |
PS01050; YJEF_C_2; 1; | |
PS51383; YJEF_C_3; 1; trigger=PRU00715; | |
Pfam | PF01256; Carb_kinase; 1; |
NCBIfam | TIGR00196; YjeF_C; 1; |
Computed features
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case <OC:Mammalia>
General | TransitM; -; 0-1; trigger=yes; |
end case
Features
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From: NNRD_BACSU (P94368) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 186 | 190 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K-x(4) | ||||||||
BINDING | 206 | 215 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | x(7)-G-x-G | ||||||||
BINDING | 149 | 155 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | x(3)-E-x(3) | ||||||||
BINDING | 104 | 104 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | |||||||||
BINDING | 216 | 216 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | D |
Additional information
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Size range | 295-413 amino acids |
Related rules | None |
Fusion | None |