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HAMAP rule MF_03158

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General rule information [?]

Accession MF_03158
Dates 13-JAN-2012 (Created)
14-MAY-2022 (Last updated, Version 11)
Name THI4
Scope
Eukaryota
Templates P32318 (THI4_YEAST); Q38814 (THI4_ARATH); Q1K6I4 (THI4_NEUCR): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THI4
case <OC:Viridiplantae>
Protein name
RecName: Full=Thiamine thiazole synthase, chloroplastic;
AltName: Full=Thiazole biosynthetic enzyme;
EC 2.4.2.60;
else
Protein name
RecName: Full=Thiamine thiazole synthase;
AltName: Full=Thiazole biosynthetic enzyme;
EC 2.4.2.60;
end case
case <OC:Saccharomycotina>
Gene name
THI4
else case <OC:Trichocomaceae>
Gene name
thiA
else case <OC:Viridiplantae>
Gene name
THI1
end case

Comments [?]

Function Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
Catalytic activity RHEA:55708: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 2 H(+) + 3 H2O + nicotinamide
EC 2.4.2.60
Cofactor Fe cation
Note: Binds 1 Fe cation per subunit.
Subunit Homooctamer.
case <OC:Viridiplantae>
Subcellular location Plastid, chloroplast.
else
Subcellular location Cytoplasm. Nucleus.
end case
Ptm During the catalytic reaction, a sulfide is transferred from #{Cys-205} to a reaction intermediate, generating a dehydroalanine residue.
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the THI4 family.

Keywords [?]

case <OC:Viridiplantae>
Chloroplast
Plastid
else
Cytoplasm
Nucleus
end case
Iron
Metal-binding
NAD
Thiamine biosynthesis
Transferase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0016763; Molecular function: pentosyltransferase activity.
GO:0005829; Cellular component: cytosol.
case <OC:Viridiplantae>
GO:0009570; Cellular component: chloroplast stroma.
end case
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.

Cross-references [?]

PRINTS PR00368; FADPNR; 1;
TIGRFAMs TIGR00292; TIGR00292; 1;

Computed features [?]

case <OC:Viridiplantae>
General TransitC; -; 0-1; trigger=yes;
end case

Features [?]

From: THI4_YEAST (P32318)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     97     98       /ligand="substrate     E-x  
BINDING     301     303       /ligand="substrate     R-M-x  
BINDING     76     76       /ligand="substrate        
BINDING     105     105       /ligand="substrate        
BINDING     170     170       /ligand="substrate        
BINDING     207     207       /ligand="substrate     D  
BINDING     237     237       /ligand="substrate     H  
BINDING     291     291       /ligand="substrate        
MOD_RES     205     205       2,3-didehydroalanine (Cys)     C  

Additional information [?]

Size range 283-383 amino acids
Related rules None
Fusion None


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