HAMAP rule MF_03158
General rule information
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Accession | MF_03158 |
Dates | 13-JAN-2012 (Created) 14-MAY-2022 (Last updated, Version 11) |
Name | THI4 |
Scope | Eukaryota |
Templates | P32318 (THI4_YEAST); Q38814 (THI4_ARATH); Q1K6I4 (THI4_NEUCR): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Viridiplantae>
Protein name |
|
else
Protein name |
|
end case
case <OC:Saccharomycotina>
Gene name |
|
else case <OC:Trichocomaceae>
Gene name |
|
else case <OC:Viridiplantae>
Gene name |
|
end case
Comments
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Function | Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. |
Catalytic activity | RHEA:55708: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 2 H(+) + 3 H2O + nicotinamide
EC 2.4.2.60 |
Cofactor | Fe cation Note: Binds 1 Fe cation per subunit. |
Subunit | Homooctamer. |
case <OC:Viridiplantae>
Subcellular location | Plastid, chloroplast. |
else
Subcellular location | Cytoplasm. Nucleus. |
end case
Ptm | During the catalytic reaction, a sulfide is transferred from #{Cys-205} to a reaction intermediate, generating a dehydroalanine residue. |
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity | Belongs to the THI4 family. |
Keywords
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case <OC:Viridiplantae>
else
end case
Gene Ontology
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GO:0005506; Molecular function: iron ion binding.
GO:0016763; Molecular function: pentosyltransferase activity.
GO:0005829; Cellular component: cytosol.
GO:0016763; Molecular function: pentosyltransferase activity.
GO:0005829; Cellular component: cytosol.
case <OC:Viridiplantae>
GO:0009570; Cellular component: chloroplast stroma.
end case
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.
Cross-references
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Computed features
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case <OC:Viridiplantae>
General | TransitC; -; 0-1; trigger=yes; |
end case
Features
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From: THI4_YEAST (P32318) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 97 | 98 | /ligand="substrate | E-x | ||||||||
BINDING | 301 | 303 | /ligand="substrate | R-M-x | ||||||||
BINDING | 76 | 76 | /ligand="substrate | |||||||||
BINDING | 105 | 105 | /ligand="substrate | |||||||||
BINDING | 170 | 170 | /ligand="substrate | |||||||||
BINDING | 207 | 207 | /ligand="substrate | D | ||||||||
BINDING | 237 | 237 | /ligand="substrate | H | ||||||||
BINDING | 291 | 291 | /ligand="substrate | |||||||||
MOD_RES | 205 | 205 | 2,3-didehydroalanine (Cys) | C |
Additional information
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Size range | 283-383 amino acids |
Related rules | None |
Fusion | None |