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HAMAP rule MF_03158

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General rule information [?]

Accession MF_03158
Dates 13-JAN-2012 (Created)
1-JUN-2023 (Last updated, Version 12)
Name THI4
Scope(s) Eukaryota
Template(s) P32318 (THI4_YEAST); Q38814 (THI4_ARATH); Q1K6I4 (THI4_NEUCR); [ Recover all ]
Triggered by HAMAP; MF_03158 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier THI4
case <OC:Viridiplantae>
Protein name RecName: Full=Thiamine thiazole synthase, chloroplastic;
AltName: Full=Thiazole biosynthetic enzyme;
Protein name RecName: Full=Thiamine thiazole synthase;
AltName: Full=Thiazole biosynthetic enzyme;
end case
case <OC:Saccharomycotina>
Gene name Name=THI4;
else case <OC:Trichocomaceae>
Gene name Name=thiA;
else case <OC:Viridiplantae>
Gene name Name=THI1;
end case

Comments [?]

FUNCTIONInvolved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5- (2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron- dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
CATALYTIC ACTIVITY Reaction=[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP- thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2- carboxylate + 2 H(+) + 3 H2O + nicotinamide; Xref=Rhea:RHEA:55708, Rhea:RHEA-COMP:14264, Rhea:RHEA-COMP:14265, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:29950, ChEBI:CHEBI:57305, ChEBI:CHEBI:57540, ChEBI:CHEBI:90873, ChEBI:CHEBI:139151; EC=;
COFACTOR Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 1 Fe cation per subunit.;
case <OC:Viridiplantae>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
PTMDuring the catalytic reaction, a sulfide is transferred from #{Cys-205} to a reaction intermediate, generating a dehydroalanine residue.
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the THI4 family.

Keywords [?]

Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
GO:0016763; Molecular function:pentosyltransferase activity
GO:0005829; Cellular component:cytosol
case <OCellular component:Viridiplantae>
GO:0009570; Cellular component:chloroplast stroma
end case
GO:0009228; Biological process:thiamine biosynthetic process
GO:0052837; Biological process:thiazole biosynthetic process

Cross-references [?]

NCBIfam TIGR00292; TIGR00292; 1;
General TransitC; -; 0-1;

Features [?]

From: THI4_YEAST (P32318)
Key From To Description Tag Condition FTGroup
BINDING 97 98 /ligand="substrate" E-x
BINDING 301 303 /ligand="substrate" R-M-x
BINDING 76 76 /ligand="substrate"
BINDING 105 105 /ligand="substrate"
BINDING 170 170 /ligand="substrate"
BINDING 207 207 /ligand="substrate" D
BINDING 237 237 /ligand="substrate" H
BINDING 291 291 /ligand="substrate"
MOD_RES 205 205 /note="2,3-didehydroalanine (Cys)" C

Additional information [?]

Size range 283-383 amino acids
Related rules None
Fusion Nter: None Cter: None

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