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Annotation rule MF_03158 |
General rule information
[?]
Accession |
MF_03158 |
Dates |
13-JAN-2012 (Created) 14-MAY-2022 (Last updated, Version 11) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Viridiplantae>
Protein name |
RecName:
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Full=Thiamine thiazole synthase, chloroplastic;
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AltName:
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Full=Thiazole biosynthetic enzyme;
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EC 2.4.2.60;
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|
else
Protein name |
RecName:
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Full=Thiamine thiazole synthase;
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AltName:
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Full=Thiazole biosynthetic enzyme;
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EC 2.4.2.60;
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|
end case
case <OC:Saccharomycotina>
else case <OC:Trichocomaceae>
else case <OC:Viridiplantae>
end case
Function |
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. |
Catalytic activity |
RHEA:55708: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 2 H(+) + 3 H2O + nicotinamide
EC 2.4.2.60
|
Cofactor |
Fe cation Note: Binds 1 Fe cation per subunit. |
Subunit |
Homooctamer. |
case <OC:Viridiplantae>
Subcellular location |
Plastid, chloroplast. |
else
Subcellular location |
Cytoplasm. Nucleus. |
end case
Ptm |
During the catalytic reaction, a sulfide is transferred from #{Cys-205} to a reaction intermediate, generating a dehydroalanine residue. |
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
Miscellaneous |
This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity |
Belongs to the THI4 family. |
case <OC:Viridiplantae>
else
end case
GO:0005506; Molecular function: iron ion binding.
GO:0016763; Molecular function: pentosyltransferase activity.
GO:0005829; Cellular component: cytosol.
case <OC:Viridiplantae>
GO:0009570; Cellular component: chloroplast stroma.
end case
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0052837; Biological process: thiazole biosynthetic process.
case <OC:Viridiplantae>
General |
TransitC; -; 0-1; trigger=yes; |
end case
From: THI4_YEAST (P32318) |
Key
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From
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To
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|
Description
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|
Tag
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|
Condition
|
|
FTGroup
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BINDING
|
|
97
|
|
98
|
|
/ligand="substrate
|
|
|
|
E-x
|
|
|
BINDING
|
|
301
|
|
303
|
|
/ligand="substrate
|
|
|
|
R-M-x
|
|
|
BINDING
|
|
76
|
|
76
|
|
/ligand="substrate
|
|
|
|
|
|
|
BINDING
|
|
105
|
|
105
|
|
/ligand="substrate
|
|
|
|
|
|
|
BINDING
|
|
170
|
|
170
|
|
/ligand="substrate
|
|
|
|
|
|
|
BINDING
|
|
207
|
|
207
|
|
/ligand="substrate
|
|
|
|
D
|
|
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BINDING
|
|
237
|
|
237
|
|
/ligand="substrate
|
|
|
|
H
|
|
|
BINDING
|
|
291
|
|
291
|
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/ligand="substrate
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|
|
|
|
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MOD_RES
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205
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205
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2,3-didehydroalanine (Cys)
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C
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Additional information
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Size range |
283-383 amino acids |
Related rules |
None |
Fusion |
None |