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HAMAP rule MF_03162

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General rule information [?]

Accession MF_03162
Dates 8-JAN-2013 (Created)
19-NOV-2022 (Last updated, Version 10)
Name RNA_methyltr_E_TRM7
Scope(s) Eukaryota
Template(s) P38238 (TRM7_YEAST); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_03162 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRM7
Protein name RecName: Full=Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase;
AltName: Full=2'-O-ribose RNA methyltransferase TRM7 homolog;
case <OC:Mammalia>
Protein name + AltName: Full=Protein ftsJ homolog 1;
end case
case <OC:Saccharomycetales>
Gene name Name=TRM7;
end case
case <OC:Mammalia>
Gene name Name=FTSJ1;
end case

Comments [?]

FUNCTIONMethylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
CATALYTIC ACTIVITY Reaction=cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 H(+) + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42396, Rhea:RHEA- COMP:10246, Rhea:RHEA-COMP:10247, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74445, ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=;
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0008175; Molecular function:tRNA methyltransferase activity
GO:0002181; Biological process:cytoplasmic translation
GO:0002128; Biological process:tRNA nucleoside ribose methylation

Cross-references [?]

Pfam PF01728; FtsJ; 1;
PIRSF PIRSF005461; 23S_rRNA_mtase; 1;

Features [?]

From: TRM7_YEAST (P38238)
Key From To Description Tag Condition FTGroup
ACT_SITE 164 164 /note="Proton acceptor" K
BINDING 55 55 /ligand="S-adenosyl-L-methionine"
G 1
BINDING 57 57 /ligand="S-adenosyl-L-methionine"
[WF] 1
BINDING 83 83 /ligand="S-adenosyl-L-methionine"
D 1
BINDING 99 99 /ligand="S-adenosyl-L-methionine"
[DN] 1
BINDING 124 124 /ligand="S-adenosyl-L-methionine"
D 1

Additional information [?]

Size range 244-534 amino acids
Related rules MF_01547
Fusion Nter: None Cter: None

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