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HAMAP rule MF_03162

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General rule information [?]

Accession MF_03162
Dates 8-JAN-2013 (Created)
19-NOV-2022 (Last updated, Version 10)
Name RNA_methyltr_E_TRM7
Scope
Eukaryota
Template P38238 (TRM7_YEAST)
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRM7
Protein name
RecName: Full=Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase;
EC 2.1.1.205;
AltName: Full=2'-O-ribose RNA methyltransferase TRM7 homolog;
case <OC:Mammalia>
Protein name
Full=Protein ftsJ homolog 1;
end case
case <OC:Saccharomycetales>
Gene name
TRM7
end case
case <OC:Mammalia>
Gene name
FTSJ1
end case

Comments [?]

Function Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
Catalytic activity RHEA:42396: cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 H(+) + 2 S-adenosyl-L-homocysteine
EC 2.1.1.205
Subcellular location Cytoplasm.
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.

Keywords [?]

Cytoplasm
Methyltransferase
S-adenosyl-L-methionine
Transferase
tRNA processing

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0008175; Molecular function: tRNA methyltransferase activity.
GO:0002181; Biological process: cytoplasmic translation.
GO:0002128; Biological process: tRNA nucleoside ribose methylation.

Cross-references [?]

Pfam PF01728; FtsJ; 1;
PIRSF PIRSF005461; 23S_rRNA_mtase; 1;

Features [?]

From: TRM7_YEAST (P38238)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     164     164       Proton acceptor     K  
BINDING     55     55       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G   1
BINDING     57     57       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [WF]   1
BINDING     83     83       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D   1
BINDING     99     99       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [DN]   1
BINDING     124     124       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D   1

Additional information [?]

Size range 244-534 amino acids
Related rules MF_01547 (RLME); MF_03163 (SPB1)
Fusion None


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