Due to maintenance work, this service will be unavailable from Mon Nov 11 17:30 until Tue Nov 12 09:00 CET. Apologies for the inconvenience.

HAMAP rule MF_03163

Send feedback

General rule information [?]

Accession	MF_03163
Dates	8-JAN-2013 (Created) 7-FEB-2023 (Last updated, Version 11)
Name	RNA_methyltr_E_SPB1
Scope(s)	Eukaryota
Template(s)	P25582 (SPB1_YEAST); Q8IY81 (SPB1_HUMAN); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_03163 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	SPB1
case <OC:Fungi>
Protein name	RecName: Full=AdoMet-dependent rRNA methyltransferase ; EC=2.1.1.-; AltName: Full=2'-O-ribose RNA methyltransferase; AltName: Full=S-adenosyl-L-methionine-dependent methyltransferase;
Gene name	Name=SPB1;
else case <OC:Vertebrata>
Protein name	RecName: Full=pre-rRNA processing protein FTSJ3; EC=2.1.1.-; AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog; AltName: Full=Protein ftsJ homolog 3; AltName: Full=Putative rRNA methyltransferase 3;
Gene name	Name=FTSJ3;
else
Protein name	RecName: Full=Putative rRNA methyltransferase; EC=2.1.1.-; AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog;
end case

Comments [?]

case <OC:Fungi>
FUNCTION	Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.
CATALYTIC ACTIVITY	Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
SUBUNIT	Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle.
else case <OC:Vertebrata>
FUNCTION	Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.
CATALYTIC ACTIVITY	Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
SUBUNIT	Interacts with NIP7.
else
FUNCTION	Probable methyltransferase involved in the maturation of rRNA and in the biogenesis of ribosomal subunits.
CATALYTIC ACTIVITY	Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
end case
SUBCELLULAR LOCATION	Nucleus, nucleolus.
SIMILARITY	Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.

Keywords [?]

S-adenosyl-L-methionine

Transferase

Gene Ontology [?]

GO:0005730; Cellular component:nucleolus
case <OCellular component:Fungi>
GO:0030687; Cellular component:preribosome, large subunit precursor
GO:0070039; Molecular function:rRNA (guanosine-2'-O-)-methyltransferase activity
GO:0008650; Molecular function:rRNA (uridine-2'-O-)-methyltransferase activity
else case <OCellular component:Vertebrata>
GO:0030688; Cellular component:preribosome, small subunit precursor
GO:0008649; Molecular function:rRNA methyltransferase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0008649; Molecular function:rRNA methyltransferase activity
end case
GO:0031167; Biological process:rRNA methylation

Cross-references [?]

Pfam	PF11861; DUF3381; 1;
Pfam	PF01728; FtsJ; 1;
Pfam	PF07780; Spb1_C; 1;
Pfam	PF02520; DUF148; 1;
PROSITE	PS50312; ASP_RICH; 0-unlimited;
PROSITE	PS50313; GLU_RICH; 0-unlimited;
PROSITE	PS50318; LYS_RICH; 0-unlimited;
General	Coiled_coil; -; 0-unlimited;

Features [?]

From: SPB1_YEAST (P25582)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

159

/note="Proton acceptor"

BINDING