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HAMAP rule MF_03163

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General rule information [?]

Accession MF_03163
Dates 8-JAN-2013 (Created)
7-FEB-2023 (Last updated, Version 11)
Name RNA_methyltr_E_SPB1
Scope
Eukaryota
Templates P25582 (SPB1_YEAST); Q8IY81 (SPB1_HUMAN): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SPB1
case <OC:Fungi>
Protein name
RecName: Full=AdoMet-dependent rRNA methyltransferase ;
EC 2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase;
AltName: Full=S-adenosyl-L-methionine-dependent methyltransferase;
Gene name
SPB1
else case <OC:Vertebrata>
Protein name
RecName: Full=pre-rRNA processing protein FTSJ3;
EC 2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog;
AltName: Full=Protein ftsJ homolog 3;
AltName: Full=Putative rRNA methyltransferase 3;
Gene name
FTSJ3
else
Protein name
RecName: Full=Putative rRNA methyltransferase;
EC 2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog;
end case

Comments [?]

case <OC:Fungi>
Function Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.
Catalytic activity RHEA:48628: a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine
Subunit Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle.
else case <OC:Vertebrata>
Function Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.
Catalytic activity RHEA:48628: a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine
Subunit Interacts with NIP7.
else
Function Probable methyltransferase involved in the maturation of rRNA and in the biogenesis of ribosomal subunits.
Catalytic activity RHEA:48628: a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine
end case
Subcellular location Nucleus, nucleolus.
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.

Keywords [?]

Methyltransferase
Nucleus
Ribosome biogenesis
rRNA processing
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0005730; Cellular component: nucleolus.
case <OC:Fungi>
GO:0030687; Cellular component: preribosome, large subunit precursor.
GO:0070039; Molecular function: rRNA (guanosine-2'-O-)-methyltransferase activity.
GO:0008650; Molecular function: rRNA (uridine-2'-O-)-methyltransferase activity.
else case <OC:Vertebrata>
GO:0030688; Cellular component: preribosome, small subunit precursor.
GO:0008649; Molecular function: rRNA methyltransferase activity.
else
GO:0008649; Molecular function: rRNA methyltransferase activity.
end case
GO:0031167; Biological process: rRNA methylation.

Cross-references [?]

Pfam PF11861; DUF3381; 1;
PF01728; FtsJ; 1;
PF07780; Spb1_C; 1;
PF02520; DUF148; 1;
PROSITE PS50312; ASP_RICH; 0-unlimited;
PS50313; GLU_RICH; 0-unlimited;
PS50318; LYS_RICH; 0-unlimited;

Computed features [?]

General Coiled_coil; -; 0-unlimited;

Features [?]

From: SPB1_YEAST (P25582)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     159     159       Proton acceptor     K  
BINDING     58     58       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G   1
BINDING     60     60       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [WF]   1
BINDING     78     78       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D   1
BINDING     94     94       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [DN]   1
BINDING     119     119       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D   1

Additional information [?]

Size range 736-1019 amino acids
Related rules MF_01547 (RLME); MF_03162 (TRM7)
Fusion None


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