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HAMAP rule MF_03172

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General rule information [?]

Accession MF_03172
Dates 15-MAR-2013 (Created)
1-JUN-2023 (Last updated, Version 17)
Name Adenylate_kinase_UMP_CMP_kin
Scope
Eukaryota
Templates P15700 (KCY_YEAST); P20425 (KCY_DICDI); P30085 (KCY_HUMAN): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
KCY
case <OC:Vertebrata>
Protein name
RecName: Full=UMP-CMP kinase;
EC 2.7.4.14;
AltName: Full=Deoxycytidylate kinase;
Short=CK;
Short=dCMP kinase;
AltName: Full=Nucleoside-diphosphate kinase;
EC 2.7.4.6;
AltName: Full=Uridine monophosphate/cytidine monophosphate kinase;
Short=UMP/CMP kinase;
Short=UMP/CMPK;
else case <OC:Fungi>
Protein name
RecName: Full=Uridylate kinase;
Short=UK;
EC 2.7.4.14;
AltName: Full=ATP:UMP phosphotransferase;
AltName: Full=Deoxycytidylate kinase;
Short=CK;
Short=dCMP kinase;
AltName: Full=Uridine monophosphate kinase;
Short=UMP kinase;
Short=UMPK;
else
Protein name
RecName: Full=UMP-CMP kinase;
EC 2.7.4.14;
AltName: Full=Deoxycytidylate kinase;
Short=CK;
Short=dCMP kinase;
AltName: Full=Uridine monophosphate/cytidine monophosphate kinase;
Short=UMP/CMP kinase;
Short=UMP/CMPK;
end case
case <OC:Vertebrata>
Gene name
CMPK1, CMPK
else case <OC:Saccharomycotina>
Gene name
URA6
else case <OC:Dictyostelia>
Gene name
pyrK
end case

Comments [?]

case <OC:Vertebrata>
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Catalytic activity RHEA:11600: ATP + CMP = ADP + CDP
EC 2.7.4.14
RHEA:25094: ATP + dCMP = ADP + dCDP
EC 2.7.4.14
RHEA:44640: a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP
EC 2.7.4.6
RHEA:18113: a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP
EC 2.7.4.6
else case <OC:Fungi>
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and dUMP as phosphate acceptors, but can also use CMP, dCMP and AMP.
else
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors.
Catalytic activity RHEA:11600: ATP + CMP = ADP + CDP
EC 2.7.4.14
RHEA:25094: ATP + dCMP = ADP + dCDP
EC 2.7.4.14
end case
Catalytic activity RHEA:24400: ATP + UMP = ADP + UDP
EC 2.7.4.14
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per monomer.
Subunit Monomer.
case <OC:Fungi>
Subcellular location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.
else case <OC:Vertebrata>
Subcellular location Nucleus. Cytoplasm. Note=Predominantly nuclear.
else
Subcellular location Cytoplasm. Nucleus.
end case
Domain Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Similarity Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.

Keywords [?]

ATP-binding
Kinase
Nucleotide-binding
Pyrimidine biosynthesis
Transferase
Cytoplasm
Nucleus

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0005634; Cellular component: nucleus.
case not <OC:Fungi>
GO:0004127; Molecular function: cytidylate kinase activity.
end case
GO:0009041; Molecular function: UMP/dUMP kinase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

PROSITE PS00113; ADENYLATE_KINASE; 1;
Pfam PF00406; ADK; 1;
PRINTS PR00094; ADENYLTKNASE; 1;
NCBIfam TIGR01359; UMP_CMP_kin_fam; 1;

Features [?]

From: KCY_YEAST (P15700)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     26     31       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-x-G-K-G-T  
BINDING     74     76       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     x-[IL]-[VL]  
BINDING     104     107       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     G-[FY]-P-R  
REGION     46     76       NMPbind        
REGION     141     151       LID        
BINDING     52     52       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     R  
case <OC:Fungi>
BINDING     111     111       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     Q  
end case
BINDING     142     142       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R  
BINDING     148     148       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     R  
BINDING     159     159       /ligand="a ribonucleoside 5'-phosphate" /ligand_id="ChEBI:CHEBI:58043     R  
BINDING     187     187       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        
case not <OC:Fungi>
From: KCY_DICDI (P20425)
BINDING     98     98       /ligand="CMP" /ligand_id="ChEBI:CHEBI:60377     N  
end case

Additional information [?]

Size range 191-231 amino acids
Related rules MF_00235 (KAD); MF_03168 (KAD2); MF_03169 (KAD3); MF_03170 (KAD4); MF_03171 (KAD1)
Fusion None


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