HAMAP rule MF_03177
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_03177 |
| Accession | MF_03177 |
| Dates | 31-JUL-2013 (Created)
19-MAY-2025 (Last updated, Version 13) |
| Name | RRM3 |
| Scope(s) |
Eukaryota Saccharomycetales |
| Template(s) | P38766; [ Recover all ] |
| Triggered by |
HAMAP; MF_03177 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RRM3 |
| Protein name | RecName: Full=ATP-dependent DNA helicase RRM3; EC=5.6.2.3; AltName: Full=DNA 5'-3' helicase RRM3; AltName: Full=rDNA recombination mutation protein 3; |
| Gene name | Name=RRM3; |
Comments
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| FUNCTION | 5' to 3' DNA replicative helicase recruited to paused replisomes to promote fork progression throughout nonhistone protein- DNA complexes, naturally occurring impediments that are encountered in each S phase where replication forks pauses. Required for timely replication of the telomere and subtelomeric DNA and for wild-type levels of telomeric silencing. Involved in DNA repair during stalled replication fork, regulation of fragile sites expression and essential for genome stability. Plays also a role in mtDNA replication. Has G- quadruplex (G4) unwinding activity and can suppress G4-induced genome instability when PIF1 levels are low. |
| CATALYTIC ACTIVITY | Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction.; EC=5.6.2.3; |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.3; |
| SUBCELLULAR LOCATION | Nucleus. Chromosome, telomere. |
| SIMILARITY | Belongs to the helicase family. PIF1 subfamily. |
Keywords
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| ATP-binding |
| Chromosome |
| DNA damage |
| DNA repair |
| DNA-binding |
| Helicase |
| Hydrolase |
| Isomerase |
| Nucleotide-binding |
| Nucleus |
| Telomere |
Gene Ontology
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| GO:0005657; Cellular component:replication fork |
| GO:0000781; Cellular component:chromosome, telomeric region |
| GO:0005524; Molecular function:ATP binding |
| GO:0043139; Molecular function:5'-3' DNA helicase activity |
| GO:0051880; Molecular function:G-quadruplex DNA binding |
| GO:0006260; Biological process:DNA replication |
| GO:0006281; Biological process:DNA repair |
| GO:0000002; Biological process:mitochondrial genome maintenance |
Cross-references
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| Pfam | PF05970; PIF1; 1; |
Features
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| From: RRM3_YEAST (P38766) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 254 | 261 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x-A-G-T-G-K-S | ||||||||
| DNA_BIND | 682 | 701 | [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F | |||||||||
Additional information
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| Size range | 588-761 amino acids |
| Related rules |
MF_03176 |
| Fusion | Nter: None Cter: None |