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HAMAP rule MF_03179

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General rule information [?]

Accession MF_03179
Dates 17-OCT-2013 (Created)
1-JUN-2023 (Last updated, Version 16)
Name GCP1_TsaD
Scope
Eukaryota
Templates P43122 (QRI7_YEAST); O22145 (OSGP2_ARATH); O05518 (TSAD_BACSU); P05852 (TSAD_ECOLI); P36175 (TSAD_MANHA); Q2FWL2 (TSAD_STAA8): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Fungi>
Identifier
QRI7
Protein name
RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial;
EC 2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
Short=t(6)A synthase;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
else case <OC:Vertebrata>
Identifier
OSGL1
Protein name
RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial;
EC 2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
Short=t(6)A synthase;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
else
Identifier
OSGP2
Protein name
RecName: Full=Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial;
EC 2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
Short=t(6)A synthase;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
end case
case <OC:Vertebrata>
Protein name
Full=O-sialoglycoprotein endopeptidase-like protein 1;
Gene name
OSGEPL1
else case <OC:Viridiplantae>
Protein name
Full=Glycoprotease 1;
Gene name
GCP1
else case <OC:Caenorhabditis>
Gene name
osgl-1
else case <OC:Saccharomycotina>
Gene name
QRI7
end case

Comments [?]

Function Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.
Catalytic activity RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234
Cofactor a divalent metal cation
Note: Binds 1 divalent metal cation per subunit.
case <OC:Vertebrata>
Subunit Monomer.
else
Subunit Homodimer.
end case
Subcellular location Mitochondrion.
Similarity Belongs to the KAE1 / TsaD family.
case not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

Acyltransferase
Mitochondrion
Metal-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0005739; Cellular component: mitochondrion.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.

Cross-references [?]

PROSITE PS01016; GLYCOPROTEASE; 1;
Pfam PF00814; Peptidase_M22; 1;
PRINTS PR00789; OSIALOPTASE; 1;
NCBIfam TIGR00329; Gcp_kae1; 1;
TIGR03723; T6A_YgjD; 1;

Computed features [?]

General TransitM; -; 0-1; trigger=yes;

Features [?]

From: QRI7_YEAST (P43122)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     170     174       /ligand="substrate     x-x-S-G-x  
BINDING     328     329       /ligand="substrate     x-N  
BINDING     145     145       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     H  
BINDING     149     149       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     H  
BINDING     361     361       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     D  
BINDING     203     203       /ligand="substrate     D  
BINDING     217     217       /ligand="substrate     [GA]  
BINDING (Optional)     221     221       /ligand="substrate     [DE]  
BINDING     360     360       /ligand="substrate     [ST]  
case <OC:Mammalia>
From: OSGL1_HUMAN (Q9H4B0)
MOD_RES     74     74       N6-acetyllysine     K  
MOD_RES     140     140       N6-acetyllysine     K  
MOD_RES     203     203       N6-acetyllysine     K  
MOD_RES     230     230       N6-acetyllysine     K  
MOD_RES     240     240       N6-acetyllysine     K  
MOD_RES     299     299       N6-acetyllysine     K  
end case

Additional information [?]

Size range 376-560 amino acids
Related rules None
Fusion None
Comments The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in E.coli, B.subtilis and many other species including fungi and plants (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by tRNA threonylcarbamoyladenosine dehydratase (TcdA).


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