HAMAP rule MF_03181

General rule information [?]

Accession	MF_03181
Dates	21-FEB-2014 (Created) 19-NOV-2022 (Last updated, Version 8)
Name	PAN3
Scope(s)	Eukaryota
Template(s)	Q7SDP4 (PAN3_NEUCR); P36102 (PAN3_YEAST); Q58A45 (PAN3_HUMAN); Q95RR8 (PAN3_DROME); G0S0Y3 (PAN3_CHATD); [ Recover all ]
Triggered by	HAMAP; MF_03181 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	PAN3
Protein name	RecName: Full=PAN2-PAN3 deadenylation complex subunit PAN3; AltName: Full=PAB1P-dependent poly(A)-specific ribonuclease; AltName: Full=Poly(A)-nuclease deadenylation complex subunit 3; Short=PAN deadenylation complex subunit 3;
Gene name	Name=PAN3;

Comments [?]

case <OC:Metazoa>
FUNCTION	Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.
else case <OC:Fungi>
FUNCTION	Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein @gn(PAB1). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by @gn(XRN1). May also be involved in post-transcriptional maturation of mRNA poly(A) tails. @gn(PAN3) acts as a positive regulator for PAN activity, recruiting the catalytic subunit @gn(PAN2) to mRNA via its interaction with RNA and with @gn(PAB1).
else
FUNCTION	Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP.
end case
case <OC:Vertebrata>
SUBUNIT	Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein @gn(PABPC1) (via PABC domain), conferring substrate specificity of the enzyme complex. Interacts with the GW182 family proteins @gn(TNRC6A), @gn(TNRC6B) and @gn(TNRC6C).
else case <OC:Metazoa>
SUBUNIT	Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein (via PABC domain), conferring substrate specificity of the enzyme complex.
else case <OC:Fungi>
SUBUNIT	Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein @gn(PAB1) (via PABC domain), conferring substrate specificity of the enzyme complex.
else
SUBUNIT	Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein (via PABC domain), conferring substrate specificity of the enzyme complex.
end case
case <OC:Metazoa>
SUBCELLULAR LOCATION	Cytoplasm, P-body.
else
SUBCELLULAR LOCATION	Cytoplasm.
end case
DOMAIN	The N-terminal zinc finger binds to poly(A) RNA.
DOMAIN	Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the @gn(PAN2) nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of @gn(PAN2) in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.
DOMAIN	The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for @gn(PAN2).
SIMILARITY	Belongs to the protein kinase superfamily. PAN3 family.

Keywords [?]

Gene Ontology [?]

case <OCellular component:Metazoa>
GO:0000932; Cellular component:P-body
GO:0010606; Biological process:positive regulation of cytoplasmic mRNA processing body assembly
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005737; Cellular component:cytoplasm
end case
case <Feature:PS50103>
GO:0046872; Molecular function:metal ion binding
end case
GO:0031251; Cellular component:PAN complex
GO:0005524; Molecular function:ATP binding
GO:0000288; Biological process:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
GO:0000289; Biological process:nuclear-transcribed mRNA poly(A) tail shortening

Cross-references [?]

PROSITE	PS50011; PROTEIN_KINASE_DOM; 0-1;
PROSITE	PS50103; ZF_C3H1; 0-1;
Pfam	PF00069; Pkinase; 1;

Features [?]

From: PAN3_HUMAN (Q58A45)

Key

From

Description

Tag

Condition

FTGroup

case <OC:Mammalia>

MOTIF

284

299

/note="PABPC-interacting motif-2 (PAM-2)"

x(9)-F-x-P-x(4)

end case

From: PAN3_YEAST (P36102)

Key

From

Description

Tag

Condition

FTGroup

case <OC:Fungi>

MOTIF

143

163

/note="PABPC-interacting motif-2 (PAM-2)"

x(13)-F-x-P-x(5)

end case

REGION

275

548

/note="Pseudokinase domain"

REGION

588

Cter

/note="Knob domain"

COILED

549

587

From: PAN3_NEUCR (Q7SDP4)

Key

From

Description

Tag

Condition

FTGroup

BINDING

352

359

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

[DE]-[YF]-[HYF]-[PA]-x(3)-[ST]

BINDING

412

413

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

x-K

BINDING

302

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

[RK]

Additional information [?]

Size range	589-887 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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