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Annotation rule MF_03181
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General rule information [?]

Accession MF_03181
Dates 21-FEB-2014 (Created)
20-NOV-2019 (Last updated, Version 6)
Name PAN3
Scope
Eukaryota
Templates Q7SDP4 (PAN3_NEUCR); P36102 (PAN3_YEAST); Q58A45 (PAN3_HUMAN); Q95RR8 (PAN3_DROME); G0S0Y3 (PAN3_CHATD): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PAN3
Protein name
RecName: Full=PAN2-PAN3 deadenylation complex subunit PAN3;
AltName: Full=PAB1P-dependent poly(A)-specific ribonuclease;
AltName: Full=Poly(A)-nuclease deadenylation complex subunit 3;
Short=PAN deadenylation complex subunit 3;
Gene name
PAN3

Comments [?]

case <OC:Metazoa>
Function Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.
else case <OC:Fungi>
Function Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein @gn(PAB1). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by @gn(XRN1). May also be involved in post-transcriptional maturation of mRNA poly(A) tails. @gn(PAN3) acts as a positive regulator for PAN activity, recruiting the catalytic subunit @gn(PAN2) to mRNA via its interaction with RNA and with @gn(PAB1).
else
Function Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP.
end case
case <OC:Vertebrata>
Subunit Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein @gn(PABPC1) (via PABC domain), conferring substrate specificity of the enzyme complex. Interacts with the GW182 family proteins @gn(TNRC6A), @gn(TNRC6B) and @gn(TNRC6C).
else case <OC:Metazoa>
Subunit Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein (via PABC domain), conferring substrate specificity of the enzyme complex.
else case <OC:Fungi>
Subunit Homodimer. Forms a heterotrimer with a catalytic subunit @gn(PAN2) to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein @gn(PAB1) (via PABC domain), conferring substrate specificity of the enzyme complex.
else
Subunit Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein (via PABC domain), conferring substrate specificity of the enzyme complex.
end case
case <OC:Metazoa>
Subcellular location Cytoplasm, P-body.
else
Subcellular location Cytoplasm.
end case
Domain The N-terminal zinc finger binds to poly(A) RNA.
Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the @gn(PAN2) nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of @gn(PAN2) in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.
The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for @gn(PAN2).
Similarity Belongs to the protein kinase superfamily. PAN3 family.

Keywords [?]


Gene Ontology [?]

case <OC:Metazoa>
GO:0000932; Cellular component: P-body.
GO:0010606; Biological process: positive regulation of cytoplasmic mRNA processing body assembly.
else
GO:0005737; Cellular component: cytoplasm.
end case
case <Feature:PS50103>
GO:0046872; Molecular function: metal ion binding.
end case
GO:0031251; Cellular component: PAN complex.
GO:0005524; Molecular function: ATP binding.
GO:0000288; Biological process: nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay.
GO:0000289; Biological process: nuclear-transcribed mRNA poly(A) tail shortening.

Cross-references [?]

PROSITE PS50011; PROTEIN_KINASE_DOM; 0-1;
PS50103; ZF_C3H1; 0-1; trigger=PRU00723;
Pfam PF00069; Pkinase; 1;

Features [?]

case <OC:Mammalia>
From: PAN3_HUMAN (Q58A45)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF (Optional)     284     299       PABPC-interacting motif-2 (PAM-2)     x(9)-F-x-P-x(4)  
end case
case <OC:Fungi>
From: PAN3_YEAST (P36102)
MOTIF (Optional)     143     163       PABPC-interacting motif-2 (PAM-2)     x(13)-F-x-P-x(5)  
end case
REGION     275     548       Pseudokinase domain        
REGION     588     Cter       Knob domain        
COILED     549     587                
From: PAN3_NEUCR (Q7SDP4)
NP_BIND     352     359       ATP     [DE]-[YF]-[HYF]-[PA]-x(3)-[ST]  
NP_BIND     412     413       ATP     x-K  
BINDING     302     302       ATP     [RK]  

Additional information [?]

Size range 589-887 amino acids
Related rules None
Fusion None